基于代谢组学技术研究凤冈锌硒茶对正常大鼠机体代谢调节规律

目的:基于代谢组学技术探究凤冈锌硒茶对正常大鼠机体代谢调节的作用机制。方法:分别给予正常大鼠口服灌胃一蒸水(10 mL/kg/d,对照组)、高剂量(1 g/kg.d)、低剂量(0.5 g/kg.d)的凤冈锌硒茶6周,取肝脏观察组织病理形态变化;取大鼠血浆检测总超氧化物歧化酶(SOD)和丙二醛(MDA)水平;采用双(三甲基硅基)三氟乙酰胺(BSTFA)衍生血浆样品后,采用气相色谱-质谱法(GC-MS)检测血浆中代谢物含量的变化。通过主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)揭示凤冈锌硒茶对大鼠代谢物轮廓的影响,并筛选差异代谢物,分析凤冈锌硒茶影响大鼠机体调节的代谢通路。结果:HE染色结果显示,对照组与给茶组大鼠肝脏无明显病理变化。给茶4周后,高、低剂量组的MDA含量均较对照组显著降低(P<0.05)。给茶后的代谢物中丁酸、L-脯氨酸、琥珀酸、肌醇、硬脂酸等较对照组差异显著,这些差异代谢物主要涉及磷酸肌醇代谢、精氨酸和脯氨酸代谢和三羧酸循环等三条代谢通路。结论:本研究初步揭示了凤冈锌硒茶通过磷酸肌醇代谢、精氨酸和脯氨酸代谢、三羧酸循环等代谢途径影响大鼠机体代谢调节的生物代谢机制,为凤冈锌硒茶的进一步研究提供理论依据。

Global Metabolic Regulation of Fenggang Zinc-Selenium Tea in Normal Rat Based on Metabolomics Technology

Objective:Based on metabolomics technology,the global mechanism of Fenggang Zinc-Selenium Tea on metabolic regulation of normal rat was explored. Method:Rat were orally administered with steamed water(10 mL/kg/d,as control),high dose(1 g/kg/d)and low dose(0.5 g/kg/d)of Fenggang Zinc-Selenium Tea for 6 weeks,respectively. The pathological changes of rat liver was observed. The SOD and MDA were detected in rat plasma. The metabolites in plasma were derived with BSTFA and then analyzed by gas chromatography-mass spectrometer(GC-MS),then the data were anylzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)revealel the effect of Fenggang Zinc-Selenium Tea on metabolite profile in rats. The differential metabolites were screend. Metaboanalyst was used to obtain the metabolic pathway. Result:HE staining results showed that there were no obvious pathological changes in the liver of the control group and the tea treatment group. After 4 weeks,the MDA content in the high and low dose groups were significantly lower than those in the control group(P<0.05). There was a significant difference in the metabolic profile of the tea treatment group compared with the control group. And the difference metabolites including the butyric acid,L-proline,succinic acid,inositol and stearic acid were screed. And these differential metabolites mainly involve three metabolic pathways including phosphoinositide metabolism,arginine and proline metabolism,and tricarboxylic acid cycle. Conclusion:This study preliminarily revealed the biological metabolic mechanism of Fenggang Zinc-Selenium Tea affecting the metabolic regulation of rat through metabolic pathways such as phosphoinositide metabolism,arginine and proline metabolism,and tricarboxylic acid cycle.This provides a theoretical basis for the further research and development of Fenggang Zinc-Selenium Tea.