| 星点设计-效应面法优选荷叶中荷叶碱与荷叶黄酮的提取工艺 |
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| 引用本文： | 王国华,张保献,聂其霞,李慧,臧琛.星点设计-效应面法优选荷叶中荷叶碱与荷叶黄酮的提取工艺[J].中国中药杂志,2008,33(20):2332-2335. |
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| 作者姓名： | 王国华 张保献 聂其霞 李慧 臧琛 |
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| 作者单位： | 中国中医科学院,中药研究所,北京,100700 |
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| 摘 要： | 目的：优化荷叶中2类有效成分的醇提工艺。方法：采用星点设计，以荷叶碱，荷叶总黄酮的提取率为指标，对2种指标进行归一化处理，采用效应面法优化荷叶的提取工艺条件。结果：确定最优提取工艺为75％～80％乙醇，提取时间120～180min，加醇量20～25倍，提取2次，荷叶碱和荷叶总黄酮提取率的实际值与预测值的偏差分别为5.53％，-6．02％。结论：采用星点设计．效应面法优选荷叶的醇提工艺，实际值与预测值吻合度高，预测性良好，是可行的。
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| 关 键 词： | 荷叶碱 总黄酮 提取工艺 星点设计 效应面优化法 总评归一法 |
Optimal extraction of nuciferine and flavone from lotus leaf based on central composite design and response surface methodology |
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| WANG Guo-hua,ZHANG Bao-xian,NIE Qi-xia,LI Hui,ZANG Chen.Optimal extraction of nuciferine and flavone from lotus leaf based on central composite design and response surface methodology[J].China Journal of Chinese Materia Medica,2008,33(20):2332-2335. |
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| Authors: | WANG Guo-hua ZHANG Bao-xian NIE Qi-xia LI Hui ZANG Chen |
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| Affiliation: | WANG Guo-hua,ZHANG Bao-xian,NIE Qi-xia,LI Hui,ZANG Chen(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China) |
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| Abstract: | Objective: To optimize the process of extracting effective constituents from lotus leaf.Method: Independent variables were ethanol concentration reflux time and solvent fold,dependent variables were extraction rates of nuciferine and flavone in lotus leaf,central composite design and response surface methodology were used for optimization of extraction of lotus leaf.Result: The optimum conditions of extraction process were 75%-80% ethanol,2-3 hours for reflux,20-25 fold solvent and 2 times for extraction.Bi... |
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| Keywords: | lotus leaf nuciferine flavone extraction central composite design response surface methodology overall desirability |
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