云药七龙天对慢性阻塞性肺病大鼠Nrf2和DJ-1调控作用研究

目的〓观察云药七龙天对慢性阻塞性肺病（chronic obstructive pulmonary disease，COPD）大鼠Nrf2和DJ-1的影响。方法 60只SD大鼠随机分为正常对照组、模型组、阳性药对照组、七龙天低、中、高剂量组，每组10只。采用气管注入脂多糖加熏香烟方法诱导COPD模型，正常组注入生理盐水。七龙天低、中、高剂量组分别灌胃给予七龙天0.35、0.7、1.4 g/kg，阳性对照组灌胃孟鲁司特片13 mg/kg。分别检测各组大鼠肺泡灌洗液中有核细胞数、肺功能指标、血清和肺组织匀浆中的Nrf2和DJ-1水平。结果 与正常组比较，模型组肺泡灌洗液中有核细胞数显著增加（P<0.05），肺功能减弱、血清和肺组织匀浆中的Nrf2和DJ-1水平降低（P<0.05）；给予七龙天药物治疗后，大鼠肺功能改善，Nrf2和DJ-1水平逐渐升高，并呈现出剂量依赖性。与模型组比较，七龙天中、高剂量组血清Nrf2和DJ-1的水平增加，差异具有统计学意义（P<0.05）。结论 七龙天可以通过调节Nrf2和DJ-1水平改善机体氧化应激平衡，从而对 COPD大鼠具有一定的治疗作用。

Study on the Regulation of Qilongtian on Nrf2 and DJ-1 in Chronic Obstructive Pulmonary Disease Rats

Objective To explore the effect of an Yunnan national drug Qilongtian on the levels of Nrf2 and DJ-1 in chronic obstructive pulmonary disease (COPD). Methods 60 SD rats were randomly divided into normal control group, model group, positive control group, Qilongtian low, medium and high dose groups, with 10 rats in each group. The COPD model was induced by tracheal injection of lipopolysaccharide plus smoked cigarettes, and normal group was injected with saline. Qilongtian low, medium and high dose treatment groups were given 0.35, 0.7 and 1.4 g/kg Qilongtian by gavage respectively, and positive control group was given 13 mg/kg Montelukast Tablets. The number of nuclear cells in the alveolar lavage fluid were recorded, lung function indexes, levels of Nrf2 and DJ-1 in serum and lung tissue homogenate were measured. Results Compared with the normal group, the number of nuclear cells in the alveolar lavage fluid in the model group was significantly increased (P<0.05), lung function was impaired, meanwhile the levels of Nrf2 and DJ-1 in serum and lung tissue homogenate were decreased(P<0.05). After Qilongtian treatment, the lung function of rats were improved, and the levels of Nrf2 and DJ-1 were gradually increased in a dose dependent manner. Compared with the model group, the changes of medium and high dose groups showed statistical significance(P<0.05). Conclusion Qidongtian could optimize the redox homeostasis in the body by regulating the levels of Nrf2 and DJ-1, which leads to a therapeutic effect on COPD rats.