Isoelectric focusing in immobilized pH gradients of a snake venom fibrinolytic enzyme |
| |
Authors: | A L Guan F S Markland |
| |
Affiliation: | Department of Biochemistry, USC School of Medicine, Los Angeles 90033. |
| |
Abstract: | A fibrinolytic enzyme with a molecular weight between 23,000 and 25,000 Da has been purified from southern copperhead snake venom. Immobilized pH gradient isoelectric focusing with an ultranarrow pH interval (pH 6.65-6.95) resolved two isoforms of the fibrinolytic enzyme that were not resolved by standard isoelectric focusing. Attempts at purification of the individual isoenzymes by semi-preparative scale IPG and elution of enzyme by macerating the gel yielded only 20-40% recovery of activity. In attempts to improve recovery, a semi-preparative IPG canal-isoelectric focusing technique has been utilized. |
| |
Keywords: | |
|
|