荞麦叶提取的总黄酮对高脂血症大鼠血管活性物质的影响

背景有研究发现养麦种子提取物具有降糖、降脂、抗氧化及抑制血小板聚集等活性,但有关荞麦叶提取的有效成分对血管活性物质的影响研究尚较缺乏.目的研究荞麦叶提取的总黄酮(totalflavonesof buchwheat leaf,TFBL)对实验性高脂血症大鼠血管活性物质的影响,探讨其调血脂及抗动脉粥样硬化(atherosclerosis,AS)的可能机制.设计完全随机对照实验研究.地点、材料和干预本实验在华北煤炭医学院药理学教研室和华北煤炭医学院附属医院同位素科完成.实验选用SD大鼠30只,清洁级Ⅱ级,体质量(220±20)g,雌雄各半.大鼠被随机分为正常对照组、高脂模型组、TFBL治疗组,每组10只.用高脂饲料诱发大鼠高脂血症,口服TFBL0.3g/(kg·d),干预治疗12周.主要观察指标TFBL对高脂血症大鼠血中血栓素B2、6-酮前列腺素F1a(6-keto-prostaglandinF1a,6-keto-PGF1a)、一氧化氮、内皮素、血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、血浆肾素活性的影响.结果TFBL治疗组高脂血症大鼠血清AngⅡ、内皮素、血栓素B2水平[(102.0±64.5),(35.6±9.1),(1775.3±318.2)ng/L]明显低于模型组[(164.2±34.0),(48.5±11.8),(2216.8±442.3)ng/L](t=2.582～2.722,P＜0.05);而6-keto-PGF1a和一氧化氮水平明显高于模型组(t=2.582,P＜0.05;4.989,P＜0.01).结论TFBL具有调节血管活性物质的作用.

Effect of totalflavones of buckwheat leaf on vasoactive substances in rats with hyperlipidemia

BACKGROUND:Research has shown that buckwheat seed has been widely applied in lowering blood lipid, blood pressure, and in preventing and treating diabetes.But studies on the influences of buckwheat leaf on vasoactive substances have not been seen untill now.OBJECTIVE:The influences of total flavones of buckwheat Ieaf(TFBL) on vasoactive substances in experimental rats with hyperlipidemia were studied.The possible mechanism of regulating blood lipid and anti-atherosclerotic (AS) of TFBL was explored.DESIGN:Completely randomly controlled experimental study.SETTING, PARTICIPANTS and INTERVENTIONS:Participants were from the Department of Pharmacology, North China Coal Medical College and the Department of Isotope, Affiliated Hospital of North China Coal Medical College.A total of 30 SD rats, cleaning grade Ⅱ, weighing(220 ± 20) g, one half of male and female.They were divided into normal group, model group and TFBL group at random and ten in each group.The hyperlipidemia model of rats was induced by high lipid diet and orally treated with TFBL for 12 W at the dosage of 0.3 g/kg per day.MAIN OUTCOME MEASURES:The effect of TFBL on serum thromboxaneB2, 6-keto-prostaglandin F1α (6-keto-PGF1α), nitric oxide, endothelin, angiotensin Ⅱ(Ang Ⅱ) and plasma rennin activity in serum with hyperlipidemia were determined.RESULTS:Serum Ang Ⅱ, endothelin and thromboxaneB2 levels in the TFBL group in rats with hyperlipidemia[(102.0±64.5), (35.6±9.1),(1775.3±318.2) ng/L] were obviously lower than those in the model group[(164.2±34.0), (48.5±11.8), (2216.8±442.3) ng/L] (t=2.582-2.722, P ＜ 0.05); but the levels of 6-keto-PGF1α and nitric oxide were higher than those in the model group( t = 2.582, P ＜ 0.05; 4.989, P ＜0.01).CONCLUSION:TFBL has the effect of regulating vasoactive substances.