| 抑制剂PI-103对786-O细胞株PI3K/Akt/mTOR信号通路的影响 |
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| 引用本文： | 邢建武,于硕鹏. 抑制剂PI-103对786-O细胞株PI3K/Akt/mTOR信号通路的影响[J]. 河南医学研究, 2013, 0(5): 649-651 |
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| 作者姓名： | 邢建武 于硕鹏 |
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| 作者单位： | [1]河南科技大学第二附属医院普外科,河南洛阳471000 [2]洛阳市第一中医院普外科,河南洛阳471000 |
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| 摘 要： | 目的:探讨抑制剂PI-103对786-O细胞株PI3K/Akt/mTOR信号通路的影响。方法:本文建立PI3K抑制剂作用786-O细胞模型,CCK-8法检测PI-103的IC50值,Western blot法检测PI-103作用后细胞内pAkt蛋白表达水平。结果:786-O细胞72 h的IC50为(9.06±0.06)μmol/L,在此浓度下,细胞内p-Akt蛋白的表达水平显著降低(P<0.01)。结论:PI-103通过抑制PI3K来调控PI3K/Akt/mTOR信号通路下游分子,此信号通路在肾细胞癌发生发展中起着重要作用,为肾细胞癌的分子靶向治疗提供理论依据。
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| 关 键 词： | PI-103 肾透明细胞癌 PI3K/Akt/mTOR |
The effect of PI-103 inhibitor on PI3K/Akt/mTOR signal pathway in 786-O cell lines |
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| XING Jian-wu;YU Shuo-peng. The effect of PI-103 inhibitor on PI3K/Akt/mTOR signal pathway in 786-O cell lines[J]. Henan Medical Research, 2013, 0(5): 649-651 |
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| Authors: | XING Jian-wu YU Shuo-peng |
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| Affiliation: | XING Jian-wu;YU Shuo-peng;General Surgical Department,The Second Affiliated Hospital of Henan University of Science ＆ Technology;General Surgical Department,The First Traditional Chinese Medical Hospital of Luoyang City; |
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| Abstract: | Objective： To explore the effect of PI3K/Akt/mTOR signal pathway in 786-O cell lines by inhibitor PI-103. Methods： Establish PI3K inhibitor 786-O cell model,CCK-8 assay IC50 values of PI-103 and Western blot assay p-Akt protein expression level. Results： 786-O cells 72 h IC50 was（ 9. 06 ± 0. 06） μmol/L,p-Akt protein expression level in the cells was significantly lower（ P 0. 01）. Conclusion： PI-103 regulates the PI3K/Akt/mTOR signal pathway through inhibition of PI3K. This signal pathway plays an important role in renal cell carcinoma,which can provide a theoretical basis for molecular targeted therapy of renal cell carcinoma. |
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| Keywords: | PI-103 renal clear cell carcinoma PI3K/Akt/mTOR |
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