固肾培元方对肾阳虚大鼠耳蜗组织Bcl-2及Bax mRNA表达的影响

目的 观察固肾培元方对肾阳虚大鼠耳蜗组织Bcl-2、Bax mRNA蛋白表达的影响。

Effect of Gushen Peiyuan Recipe on Bcl-2 and Bax mRNA Expressions in Cochlea Tissue of Rats with Shen-Yang Deficiency Syndrome

Objective To study the effect of Gushen Peiyuan Recipe （GPR） on the expression of Bcl-2 and Bax mRNA in Shen-yang-deficiency （SYD） rats suffering from cochlea apoptosis, thus providing a theoretical basis to the treatment and prevention of sensorineural hearing loss and fill Chinese medine＇s theory of kidneyear-correlation with new substance. Methods Rats were induced into experimental SYD animal models by injecting cetacort into their buttocks. Rats in the blank and model groups were given 10 mL/kg normal saline by gastrogavage, and 31 g/kg, 15.5 g/kg and 7.5 g/kg GPR were given to the rats in the high, medium and low dose groups by gastrogavage respectively. RT-PCR was adopted to detect the mRNA expressions of Bcl-2 and Bax. Results Levels of Bcl-2 mRNA expression and Bcl-2/Bax enhanced, and mRNA expression of Bax attenuated in the model rats after GPR treatment, the Bcl-2/Bax ratio increased, showing insignificant difference when compared with the blank control group （ P 〉0.05）. Conclusion GPR plays a significant role in regulating the mRNA expressions of Bcl-2 and Bax, therefore improving the hearing of SYD rats and protecting the structure and function of cochlea.