| 高效液相色谱法测定间尼索地平不同对映体在血浆中的蛋白结合率 |
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| 引用本文： | 李敏,王春英,景秀娟,段坤峰,张兰桐. 高效液相色谱法测定间尼索地平不同对映体在血浆中的蛋白结合率[J]. 中国医院药学杂志, 2011, 0(3) |
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| 作者姓名： | 李敏 王春英 景秀娟 段坤峰 张兰桐 |
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| 作者单位： | 河北医科大学药学院药物分析教研室;华北煤炭医学院附属医院药剂科; |
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| 基金项目： | 国家自然科学基金项目(编号:30840098); 河北省自然科学基金资助项目(编号:C2008001073) |
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| 摘 要： | 目的:建立间尼索地平不同对映体在大鼠血浆、人血浆和牛血清白蛋白中蛋白结合率的测定方法,并计算不同种属血浆蛋白的相关参数。方法:采用平衡透析法测定蛋白结合率,用高效液相色谱法测定血浆中药物总浓度及游离药物浓度。结果:R-与S-间尼索地平的血浆蛋白结合率分别为:大鼠血浆为(97.4±8.2)%、(93.0±13.4)%;人血浆为(90.8±10.5)%、(89.2±9.9)%;牛血清白蛋白为(95.3±8.7)%、(91.0±5.7)%。最低定量下限为0.01ng。结论:在体外间尼索地平不同对映体与大鼠血浆、人血浆和牛血清白蛋白结合率很高,R-间尼索地平的结合率及蛋白结合药物的表观最大能力βp均较S-间尼索地平高。随着药物血浆浓度升高,R-间尼索地平结合率下降,S-间尼索地平结合率升高,均有一定的浓度依赖性。
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| 关 键 词： | 间尼索地平 对映体 蛋白结合率 平衡透析法 高效液相色谱法 |
Determination of the protein binding rates of m-nisoldipine enantiomers with different genera of plasma by HPLC |
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| LI Min,,WANG Chun-ying,JING Xiu-juan,DUAN Kun-feng,ZHANG Lan-tong. Determination of the protein binding rates of m-nisoldipine enantiomers with different genera of plasma by HPLC[J]. Chinese Journal of Hospital Pharmacy, 2011, 0(3) |
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| Authors: | LI Min WANG Chun-ying JING Xiu-juan DUAN Kun-feng ZHANG Lan-tong |
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| Affiliation: | LI Min1,2,WANG Chun-ying1,JING Xiu-juan1,DUAN Kun-feng1,ZHANG Lan-tong1 (1.Dept.of Pharmaceutical Analysis,School of Pharmacy,Hebei Medical University,Hebei Shijiazhuang 050017,China,2.Department of pharmacy,affiliated hospital of North China Coal Medical College,Hebei Tangshan 063000,China) |
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| Abstract: | OBJECTIVE A HPLC method was established to determine the protein binding rates of m-nisoldipine enantiomers in human plasma,rat plasma,bovine serum albumin(BSA),and to calculative the correlate parameters of m-nisoldipine enantiomers to different genera plasma proteins.METHODS The binding rates of m-nisoldipine enantiomers with different genera plasma proteins were determined by equilibrium dialysis method.The concentrations of m-nisoldipine enantiomers were assayed by HPLC.RESULTS The binding rates of R-,S... |
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| Keywords: | m-nisoldipine enantiomers plasma protein binding rate equilibrium dialysis method high performance liquid chromatography |
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