镁锌合金在体外对MC3T3-E1细胞增殖、分化和矿化的影响

目的 观察镁锌合金(Mg-zn)在体外的降解及对小鼠前成骨细胞(MC313-E1)增殖、分化和矿化的影响,探讨Mg-Zn成为新型骨科内植物材料的可行性.方法 Mg-Zn和纯镁(Mg)试样置入模拟体液(SBF)中,3 d后通过电化学方法和静态浸泡失重的方法来测定其腐蚀电位及腐蚀速率;在Mg-Zn上接种MC3T3-E1细胞,采用噻唑蓝(MTT)比色法检测1、3、5、7、9 d时细胞增殖活性,采用碱性磷酸酶(ALP)检测试剂盒检测14、28 d时细胞ALP活性,并用四环素荧光染色检测14d时矿化结节.与左旋聚乳酸(PLLA)作对照研究.结果 Mg-Zn的腐蚀电位增高,腐蚀速率降低;培养第5、7、9天后,Mg-Zn成骨细胞增殖活性明显高于PLLA(P<0.01);14、28 d时,Mg-Zn ALP活性明显高于PuA(P<0.01);Mg-Zn矿化结节面积明显大于PLLA(P<0.01).结论 Mg-Zn明显改善了Mg的耐腐蚀性能,同时能促进MC333-E1细胞的增殖、分化和矿化功能,具有良好的生物相容性.

In vitro effects of Mg-Zn alloy on proliferation, differentiation and mineralization in MC3T3-E1 cells

Objective To observe the degradation of a novel biodegradable Mg-Zn alloy in vitro, investigate the effects of Mg-Zn alloy on proliferation, differentiation and mineralization in MC3T3-E1 cells, and try to find out the possibility of Mg-Zn alloy to be a new type of bone surgery implant material. Methods The corrosion electric potential and rate of Mg-Zn alloy in a phosphate buffered simulated body fluid (SBF) were investigated by electrochemical testing and weight loss experiment after 3 days;Preosteoblast MC3T3-E1 cells were inoculated onto the Mg-Zn alloy scissel. The proliferation of MC3T3-E1 cells was measured by MTT method after 1, 3, 5, 7 and 9 days;The differentiation was measured through alkaline phosphatase (ALP) kit after 14 and 28 days;The mineralization was measured through ambramycin staining after 14 days. Pory-L-lactie acid (PLLA) was used as the conterparts. Results The corrosion electric potential of Mg-Zn alloy were increased and the corrosion rate steped down;The osteoblasts proliferation on Mg-Zn alloy was significantly increased as compared with that on PLLA after 5, 7 and 9 days ( P < 0. 01);After 14 and 28 days of culture, the ALP activity on Mg-Zn alloy was significantly stronger than that on PLLA (P < 0.01);After 14 days of culture, the mineralization nodes area in Mg-Zn alloy was greater than that on PLLA (P < 0.01). Conclusion Mg-Zn obviously improved the corrosion resistance of Mg and can promote the proliferation, differentiation and mineralization in MC3T3-E1 cells, so the Mg-Zn alloy is considered to have favourable biocompatibility.