HPLC测定复方乙酰水杨酸片中3组分的含量

目的同时测定复方乙酰水杨酸片中乙酰水杨酸、咖啡因、非那西丁的含量。方法采用HPLC，色谱柱：Symmetry C18柱（4．6mm×150mm，5um），流动相为pH6．5的磷酸二氢钾缓冲液-乙腈（78：22），流速为1．0mL·min^-1，检测波长为270nm。结果3组分分别在8．80～70．42ug·mL^-1（r=0．9999）、1．42～11．34ug·mL^-1（r=0．9999）、6．12～48．92ug·mL^-1。（r=0．9999）内成良好线性关系，平均回收率分别为101．2％（RSD=0．7％，n=6）、99．1％（RSD=0．8％，n=6）、100．3％（RSD=0．7％，n=6）。结论本法可不经分离，直接测定复方乙酰水杨酸片中3组分的含量。

Determination of Three Components in Compound Aspirin Tablets by HPLC

OBJECTIVE To determine acetyl salicylic acid,caffeine and phenacetin in compound aspirin tablets. METHODS The chromatographic conditions included Symmetry C18 column（4.6 mm× 150 mm, 5 um）. The mobile phase was phosphate salt buffer （pH6.5）-acetonitrile （78： 22）. The flow rate was 1.0 mL·min^-1. The detection wavelength was 270 nm. RESULTS Acetyl salicylic acid,caffeine and phenacetin had good linear relationship in the range of 8.80 - 70.42 ug·mL^-1 （ r = 0. 999 9 ）, 1.42- 11.34 ug·mL^-1 （ r = 0. 999 9） and 6.12-48.92ug·mL^-1 （r = 0. 999 9）. Their recoveries were 101.2% （ RSD = 0.7%, n = 6）, 99.1% （RSD =0.8% ,n =6） and 100.3% （RSD =0.7% ,n=6） ,respectively. CONCLUSION This method could be used for the determination of three components in compound aspirin tablets without separation.