A growth-promoting factor existing on the body surface of rats |
| |
| Authors: | H Namiki H Ogata N Koga S Kusunoki |
| |
| Affiliation: | 1. College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road, Qingdao, Shandong Province 266003, PR China;2. Qingdao National Laboratory for Marine Science and Technology, Laboratory of Marine Drugs & Biological products, Qingdao 266237, PR China;1. Department of Biology, University of California, Riverside, CA 92521, USA;2. Department of Physical Education, Federal University of Minas Gerais, Brazil;1. II. Medizinische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, 81675 Munich, Germany;2. Department of Anesthesiology, Center of Anesthesiology and Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany |
| |
| Abstract: | Effects of water-soluble matter adhering to rat hairs on fibroblasts were examined. The dialysate of the wash water of rat hairs significantly enhanced the cell proliferation of both diploid human dermal fibroblasts (DHDF) and diploid rat fibroblasts (DRDF). The cell growth-promoting activity was partially purified by a gel filtration column chromatography. The activity permeates through a ultrafiltration membrane (M.W. cut off: 500). Analyses of its chemical nature show that it is soluble in water, dimethyl sulfoxide or acetonitrile, insoluble in other organic solvents examined, stable to heat or pH shock, and resistant to a bacterial protease. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
