roTOR信号在KA诱导幼鼠癎性发作后的表达变化研究

目的：研究哺乳动物雷帕霉索靶蛋白（mammalian target of rapamycin，mTOR）信号在幼鼠癎性发作后的表达变化。方法：海人藻酸（KA）诱导幼鼠癎性发作，根据疝性发作后不同的时间点（1h、3h、16h、24h、3d、5d、1w、2w、6w）进行取材，从大脑中分离KA注射侧海马组织，提取全蛋白进行westernblot检测观察癎性发作后mTOR信号通路活化的标志物核糖体蛋白56（p-S6）蛋白的表达变化。免疫组织化学检测海马区P-S6免疫阳性细胞的表达。结果：KA海马内注射1h、3h、16h、24h，3d，5d，1w，2w，6w大鼠海马组织进行westernblot检测，发现在急性期P-S6的表达量呈现持续升高的表达变化。KA致癎1w后，海马区p-S6阳性细胞表达相对于手术对照组明显增高（76±12VS25±5）。在大鼠大脑皮层神经元亦可见到致癎后大量表达的p-S6阳性神经元（88±13 VS 15±3）。结论：mTOR信号活化标志物p-S6蛋白在KA诱导幼鼠癎性发作后表达明显增加，抑制mTOR信号的异常活化或许成为预防癫癎形成的新靶点。

The study of the expression of mTOR signaling in immature rats after epileptic seizures

Objective：To study the expression of mammalian target of rapamycin（mTOR） signaling in immature rats after epileptic seizures. Methods： The hippocampus were separated in kainate （KA） in- jected side after KA-indueed seizures in immature rats according to different time （ lh, 3h, 16h, 24h, 3d, 5d, l w,2w,6w）. While proteins were extracted, Western Blotting Detection observed the expression al- terations of p-S6 protein as the roTOR signaling pathway activation markers. Immunohistoehemistry de- tected the expression of p-S6 hippocampus immunoreactive cells. Results： The expression of p-S6 protein in hippocampal tissue remained elevated after KA injection to therats for lh,3h,16h,24h,3d,Sd,lw,2w, 6w. After KA epileptic lW, the p-S6-positive cells in the hippocampus was significantly higher compared with those in to the surgical control group （76 ± 12 vs 25 ± 5）. P-S6 positive neurons in rat cortical neurons after epileptic could also observe a lot of expressions （88 ± 13 vs 15 ± 3）. Conclusion：mTOR signal activation markers p-S6 protein expression significantly increased in the KA-induced seizures in rats after inhibition of aberrant activation of mTOR signaling, which may serve as a new target for the preven- tion of the formation of epilepsy.