Investigation of the miRNA146a and miRNA155 gene expression levels in patients with multiple sclerosis |
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| Affiliation: | 1. Department of Medical Biology , Faculty of Medicine, Ege University, Izmir, Turkey;2. Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran;3. Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Neurology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;5. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;1. Department of Neurosurgery, The Canberra Hospital, Garran, ACT, Australia;2. Department of Neurosurgery, The Royal Melbourne Hospital, Parkville, Victoria, Australia;1. Department of Neurological Surgery, University of Texas Southwestern, 5323 Harry Hines Blvd, Dallas, TX 75390, USA;2. Department of Neurology and Neurotherapeutics, University of Texas Southwestern, 5323 Harry Hines Blvd, Dallas, TX 75390, USA;1. The Warren Alpert School of Medicine, Brown University, Providence, RI, United States;2. Department of Neurosurgery, Rhode Island Hospital, Providence, RI, United States;3. Department of Psychiatry, University of Illinois at Chicago, Chicago, IL, United States;1. Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, Level 11, Singapore 119228, Singapore;2. Division of Neurosurgery, Department of Surgery, National University Hospital, National University Health System, 1E Kent Ridge Road, Level 11, Singapore 119228, Singapore;3. Department of Neurosurgery, Khoo Teck Puat Hospital, Alexandra Health Private Limited, National University Health System, 90 Yishun Central, Singapore 768828, Singapore;4. Neurosurgery Service, Ng Teng Fong General Hospital, Jurong Health Campus, National University Health System, 1 Jurong East Street 21, Singapore 609606, Singapore;5. Department of Neurosurgery, Addenbrooke’s Hospital, University of Cambridge, Hills Rd, Cambridge CB2 0QQ, United Kingdom |
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| Abstract: | Multiple sclerosis (MS) is a chronic inflammatory disease and the most common neurodegenerative status. MicroRNAs play an important role in macrophage response to inflammatory processes, and alterations in miRNA levels trigger the inactivation of specific T lymphocytes. As a result, these factors can lead to autoimmune diseases such as MS. Therefore, to determine the role of MicroRNA-146a and MicroRNA-155 in MS patients, their expression levels in serum of MS patients were compared with healthy controls.In this study, the expression levels of MicroRNA-146a and MicroRNA-155 in 30 serum samples of MS and healthy patients as a control group. MicroRNA extraction and cDNA synthesis was performed according manufacture protocols. The expression levels of MicroRNAs were evaluated by Real Time-PCR.MicroRNA-146a and MicroRNA-155 levels were increased in patients with MS compared to controls. The results demonstrated that EDSS score are increased with increasing level of MicroRNA-146a and MicroRNA-155. ROC curve analysis showed that the area under curve (AUC) was significant for MicroRNA-146a and MicroRNA-155.Increased expression levels of MicroRNA-146a and MicroRNA-155 may be associated with the pathogenesis of MS disease. If this study is conducted in a larger sample population and the above results can be used to identify patients or control patients who are under medical care. |
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| Keywords: | Multiple Sclerosis (MS) MicroRNA-146a MicroRNA-155 Novel inflammatory biomarkers |
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