| 气管内反复滴入脂多糖法建立大鼠慢性阻塞性肺疾病模型 |
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| 引用本文： | 刘君波,黄梦珊,余晨曦,王砚,唐法娣. 气管内反复滴入脂多糖法建立大鼠慢性阻塞性肺疾病模型[J]. 中国实验动物学报, 2011, 19(2): 129-133,191. DOI: 10.3969/j.issn.1005-4847.2011.02.010 |
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| 作者姓名： | 刘君波 黄梦珊 余晨曦 王砚 唐法娣 |
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| 作者单位： | 浙江大学医学院呼吸药物研究实验室,浙江,杭州,310058 |
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| 基金项目： | 浙江省中医药重点项目研究计划资助项目 |
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| 摘 要： | 目的评价脂多糖(LPS)诱发大鼠慢性阻塞性肺疾病(COPD)模型的可行性。方法气管内滴入脂多糖或生理盐水,每周1次,共8周。测定大鼠的气道阻力(RL)和肺动态顺应性(Cdyn),计数大鼠支气管肺泡灌洗液(BALF)中白细胞总数及分类,肺组织病理切片行HE和AB-PAS染色,并测定肺组织粘蛋白(MUC5AC)的含量。结果模型组大鼠RL明显升高(87.5%),Cdyn显著下降(16.4%);BALF中白细胞总数及分类中的中性粒细胞、淋巴细胞和单核巨噬细胞数均明显高于对照组;光镜下可见病变呈慢性支气管炎及肺气肿样改变。结论反复气管内滴入LPS可用于制备大鼠COPD模型,其肺功能、BALF细胞学及病理学改变符合人类COPD表现,可以用于实验研究。
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| 关 键 词： | 慢性阻塞性肺疾病 脂多糖 大鼠 模型 |
Establishment of a rat model of chronic obstructive pulmonary disease by intratracheal instillation of lipopolysaccharide |
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| LIU Jun-bo,HUANG Meng-shan,YU Chen-xi,WANG Yan,TANG Fa-di. Establishment of a rat model of chronic obstructive pulmonary disease by intratracheal instillation of lipopolysaccharide[J]. Acta Laboratorium Animalis Scientia Sinica, 2011, 19(2): 129-133,191. DOI: 10.3969/j.issn.1005-4847.2011.02.010 |
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| Authors: | LIU Jun-bo HUANG Meng-shan YU Chen-xi WANG Yan TANG Fa-di |
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| Affiliation: | LIU Jun-bo,HUANG Meng-shan,YU Chen-xi,WANG Yan,TANG Fa-di(Zhejiang Research Laboratory of Respiratory Drugs,College of Medicine,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | Objective To establish and evaluate the feasibility of a rat model of chronic obstructive pulmonary dis-ease(COPD) induced by intratracheal instillation of lipopolysaccharide(LPS).Methods LPS or saline was instilled in-tratracheally in rats once a week for consecutive 8 weeks.The rats were anesthetized and taken for the measurement of lung resistance(RL) and dynamic compliance(Cdyn).Total and differential white blood cell counts in bronchoalveolar lavage fluid(BALF) were carried out.The pathological changes... |
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| Keywords: | Chronic obstructive pulmonary disease Lipopolysaccharide Rat Model |
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