| miR-34a抑制人鼻咽癌细胞生长与侵袭 |
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| 引用本文： | 黄远见,肖娟,张志伟,罗志强.miR-34a抑制人鼻咽癌细胞生长与侵袭[J].南华大学学报(医学版),2014,42(3):251-254. |
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| 作者姓名： | 黄远见 肖娟 张志伟 罗志强 |
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| 作者单位： | 1.南华大学附属第二医院耳鼻咽喉科，湖南 衡阳 421001；2.南华大学肿瘤研究所 |
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| 摘 要： | 目的探讨miR-34a在人鼻咽癌CNE2细胞中的生物学功能。方法采用MTT、Transwell侵袭实验、细胞划痕实验分别检测过表达miR-34a对人鼻咽癌CNE2细胞的生长与侵袭作用。结果 MTT检测发现,过表达miR-34a可抑制人鼻咽癌CNE2细胞的生长增殖;细胞划痕和Transwell侵袭实验显示,在鼻咽癌CNE2细胞中过表达miR-34a 48 h后划痕愈合率为40.3%±9.0%,与对照组（76.3%±6.8%）比较,能明显减缓CNE2细胞划痕的愈合,转染miR-34a mimics 48 h后穿过基底膜的细胞数为71±10,与对照组（147±5）比较,能明显减缓CNE2细胞侵袭能力（P〈0.01）。结论 miR-34a可抑制鼻咽癌CNE2细胞生长与侵袭。
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| 关 键 词： | miR-34a 鼻咽癌 生长与侵袭 |
| 收稿时间： | 2013/9/29 0:00:00 |
The Mechanism of the MiR-34a Inhibiting the NasopharyngealCarcinoma Cells Growth and Invasion |
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| HUANG Yuanjian,XIAO Juan,ZHANG Zhiwei,et al.The Mechanism of the MiR-34a Inhibiting the NasopharyngealCarcinoma Cells Growth and Invasion[J].Journal of Nanhua University(Medical Edition),2014,42(3):251-254. |
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| Authors: | HUANG Yuanjian XIAO Juan ZHANG Zhiwei |
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| Affiliation: | Department of Otorhinolaryngology,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China |
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| Abstract: | ObjectiveTo analyse the mechanism of the role of miR-34a in the nasopharyngeal carcinoma cells.MethodsCell growth was evaluated by MTT.Cell invasion potential was evaluated by scratch test and transwell assay.ResultsThe MTT assay demonstrated that after overexpressing miR-34a in nasopharyngeal carcinoma cells,cellular viability was significantly reduced (P<0.05).The Transwell invasion chamber assay illustrated that after increasing the expression of miR-34a,the number of cells passing through the Transwell chamber was significantly reduced (P<0.01).ConclusionsmiR-34a can inhibit nasopharyngeal carcinoma cell proliferation,invasion and induced apoptosis. |
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| Keywords: | miR-34a nasopharyngeal carcinom proliferation and invasion |
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