异氧硝化细菌处理氨氮废水及微生物群落结构分析

从生物陶粒反应器中筛选出6株异养硝化细菌,采用乙酸钠-氯化铵培养基培养细菌进行硝化特性研究,经过12d好氧培养,6株异养硝化细菌对COD的去除率在45%以上,总氮和氨氮最终去除率在60%以上,并且具有产生NO_x-N的硝化性能.应用PCR-DGGE(denaturing gradient gel elelectrophoresis)方法研究了SBR反应器种群结构动态变化规律,PCR-DGGE图谱分析表明,微生物多样性与废水的处理效果出现协同变化的特征,1 d与15 d微生物群落结构相似程度最高为75%,1 d与30 d相似性最低为52.94%,15 d与30 d相似性为70.59%,3株筛选的异氧硝化细菌wgy1、wgy3、wgx5成为SBR反应器的优势菌群.

Heterotrophic Nitrobacteria Disposal of the Wastewater of NH4-N and Analysis of the Diversity of Microbial Species

6 heterotrophic nitrobacteria are screened from bio-ceramic reactors.And sodium acetate and am- monium chloride are used as carbon and nitrogen source to foster bacteria for the characteristic study of nitrifi- cation.COD removal rate could reach over 45%,TN and NH_4-N removal rate could reach over 60 %,and al- so it has the nitrification function of producing NO_x-N.By the method of PCR-DGGE,this paper studies the dynamic changes of the SBR reactor.The PCR-DGGE profiles show that shift of microbial diversity corre- sponds to the effect of nitrate removal.1 d and the 15 d similarity is 75%,which microbial diversity is the highest.1 d and the 30 d similarity is the lowest which is just 52.9496,15 d and the 30 d.similarity is 70.59%.wgyl,wgy3 and wgx5 are dominated bacteria in sequencing batch reactor.