| 冷冻电镜单颗粒重构中病毒三维显示的校正 |
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| 引用本文： | 李晶,李鲲鹏,柳正,张景强,张勤奋,李茵茵.冷冻电镜单颗粒重构中病毒三维显示的校正[J].电子显微学报,2009,28(5):410-413. |
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| 作者姓名： | 李晶 李鲲鹏 柳正 张景强 张勤奋 李茵茵 |
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| 作者单位： | 1. 中山大学生物防治国家重点实验室,广州,510275;昆明总医院医学工程中心,昆明,650032
2. 中山大学生物防治国家重点实验室,广州,510275 |
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| 基金项目： | 国家自然科学基金资助项目，中国博士后科学基金资助项目 |
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| 摘 要： | 利用冷冻电镜显微技术和单颗粒三维重建方法获得BmCPV（家蚕质多角体）;CSBV（中蜂囊状幼虫病病毒）;C6/36DNV（C6/36浓核病毒）的三维结构体数据,用空间低通滤波对体数据矢量场进行处理并进行三维显示,较原来的显示,提高了信噪比,增强了显示稳定性与显示质量。病毒每个表面细节和轴上突起更加清晰可见。
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| 关 键 词： | 冷冻电子显微技术 三维重构 低通滤波 三维显示 病毒 |
Correction three-dimensional display of virus in electron cryo-microscopy single particle reconstruction |
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| LI Jing,LI Kun-peng,LIU Zheng,ZHANG Jing-qiang,ZHANG Qin-fen,LI Yin-yin.Correction three-dimensional display of virus in electron cryo-microscopy single particle reconstruction[J].Journal of Chinese Electron Microscopy Society,2009,28(5):410-413. |
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| Authors: | LI Jing LI Kun-peng LIU Zheng ZHANG Jing-qiang ZHANG Qin-fen LI Yin-yin |
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| Affiliation: | LI Jing , LI Kun-peng , LIU Zheng , ZHANG ZHANG , LI Jing-qiang , Qin-fen Yin-yin(1.State Key Laboratory for Bioeontrol, School of Life Science, Sun Yat-sen University, Guangzhou Guangdong 510275; 2. Department of Equipment, Kunming General Hospital, Kunming Yunnan 650032,China) |
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| Abstract: | We present a novel method using a low pass filter to correct three-dimensional (3D) display in electron cryo-microscopy single particle reconstruction. SNR is increased after passing the filter. As a result, the methed has improvied both stability and performance of 3D display our method can be applied to process 1.3 nm(resolution) BmCPV (Bombyx mori Cytoplasmic Polyhedrosis Virus), 2.5 nm CSBV (Chinese Sacbrood Bee Virus), and 1.4 nm C6/36DNV(Bensonucleosis Virus) with the satisfied results. |
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| Keywords: | electron cryo-microscopy 3D reconstruction low pass filter virus |
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