| 硒对蚕豆胚根抗氧化酶系统的影响 |
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| 引用本文： | 张苗苗,韩善华,王亚男,杜凤移,王阚,王安喜. 硒对蚕豆胚根抗氧化酶系统的影响[J]. 四川师范大学学报(自然科学版), 2010, 33(2). DOI: 10.3969/j.issn.1001-8395.2010.02.025 |
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| 作者姓名： | 张苗苗 韩善华 王亚男 杜凤移 王阚 王安喜 |
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| 作者单位： | 四川师范大学,生命科学学院,四川,成都,610066 |
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| 基金项目： | 四川师范大学科研启动基金(037054)对本文的资助 |
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| 摘 要： | 采用水培方法,研究不同浓度Se4+对蚕豆胚根抗氧化酶系统的影响.结果表明,随着Se4+浓度的升高,SOD活性一直升高,而CAT活性下降;当Se4+浓度低于0.01μmol/L时POD活性随着浓度升高而下降,Se4+浓度高于0.01μmol/L时POD活性开始升高;Se4+浓度低于0.005μmol/L时MDA含量随浓度升高而降低,Se4+浓度高于0.01μmol/L时MDA含量开始升高.对POD同功酶的聚丙烯酰胺凝胶电泳结果分析表明,不同浓度的Se4+处理蚕豆根POD同功酶没有特异性条带的表达,但活性强弱不同,有的带已经消失了.
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| 关 键 词： | 硒 抗氧化酶系统 同功酶 蚕豆 |
The Effect of Selenium on the Antioxidaut Enzyme System of Radicle Root of Vicia Faba |
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| ZHANG Miao-miao,HAN Shan-hua,WANG Ya-nan,DU Feng-yi,WANG Kan,WANG An-xi. The Effect of Selenium on the Antioxidaut Enzyme System of Radicle Root of Vicia Faba[J]. Journal of Sichuan Normal University(Natural Science), 2010, 33(2). DOI: 10.3969/j.issn.1001-8395.2010.02.025 |
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| Authors: | ZHANG Miao-miao HAN Shan-hua WANG Ya-nan DU Feng-yi WANG Kan WANG An-xi |
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| Affiliation: | ZHANG Miao-miao,HAN Shan-hua,WANG Ya-nan,DU Feng-yi,WANG Kan,WANG An-xi(College of Life Science,Sichuan Normal University,Chengdu 610066,Sichuan) |
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| Abstract: | The authors investigated antioxidant enzyme system in the radicle of Vicia Faba treated with different concentrations of Se~(4+).The results indicated that SOD activity increased and CAT acvtivity decreased with the increase of the concentration of Se~(4+);if the concentration was lower than 0.01 μmol/L POD activity decreased with the increase of the concentration;when the concentration was lower than 0.005 μmol/L Se~(4+) the content of MDA decreased as concentration increased.By polyacrylancide gel electrophoresis(PAGE),different concentrations of Se~(4+) did not induce the expression of special strips for POD isoenzymes,but the activities were different from each other,some strips disappeared. |
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| Keywords: | selenium antioxidant enzyme system isoenzyme Vicia Faba |
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