高效液相色谱法同时测定脑血栓片中丹参素、原儿茶醛、芍药苷、阿魏酸和丹酚酸B的含量

目的:建立同时测定脑血栓片中丹参素、原儿茶醛、芍药苷、阿魏酸和丹酚酸 B 含量的方法。方法:采用反相高效液相色谱法,色谱柱:Zorbax Extend-C_(18)柱(4.6 mm×150 mm,5 μm),流动相:1%甲酸甲醇溶液(A)-1%甲酸溶液(B),梯度洗脱[0～8 min,A-B(8:92);8～23 min,A-B(8:92)→A-B(40:60)],流速1.0 mL·min~(-1),柱温30℃,检测器:DAD 检测器,检测波长为280 nm。结果:丹参素、原儿茶醛、芍药苷、阿魏酸和丹酚酸 B 的线性范围分别为0.072～0.72μg(r=0.9999),0.04～0.4μg(r=0.9999),0.032～0.32μg(r=0.9999),0.002～0.02μg(r=0.9993),0.08～0.8μg(r=0.9997);平均加样回收率(n=3)分别为97.2%,97.7%,98.3%,98.3%,96.7%。结论:本方法简便快速,结果准确可靠,适用于脑血栓片的质量控制。

HPLC determination of danshensu, protocatechuic aldehyde, paeoniflorin, ferulic acid and salvianolic acid B in Naoxueshuan tablets