普通环境长爪沙鼠呼吸道菌群的分离鉴定

目的调查普通环境长爪沙鼠呼吸道细菌及支原体携带情况,为制定长爪沙鼠微生物学等级标准提供依据。方法对普通环境饲养的65只长爪沙鼠进行解剖,取气管分泌物接种血琼脂,分别放入普通培养箱和5%CO2培养箱中培养48 h,分离菌株后同时进行生化分析和PCR测序鉴定,以期准确判断分离菌株所属菌属;气管浸液提取DNA,用支原体特异性引物进行PCR检测,并分别计算检出率。结果本研究共检出22种细菌及支原体。不同细菌、支原体感染率相差很大,阳性率在1.5%(1/65)到64.6%(42/65)之间,其中部分细菌为大鼠和小鼠致病菌。结论本研究为长爪沙鼠的微生物学等级标准的制定提供了参考数据。

Isolation and Identification of Respiratory Tract Flora of Mongolian Gerbils from Conventional Facility

Objective To investigate the respiratory tract flora and mycoplasma status in Mongolian gerbils from a conventional facility,and provide basic information for the developing microbiologic testing standards of gerbils.Methods Sixty-five gerbils from conventional facility of Capital Medical University were dissected to obtain the tracheal secretion,and then the samples were seeded in blood agar base,cultured in common or 5% CO2 incubator for 48 h,separately.In order to accurately identify the genus to which the isolated strain belongs,biochemical analysis and PCR sequencing were performed.On the other hand,DNA was isolated from tracheal lixivium,and mycoplasma DNA was amplified by specific primer.The detection rate was calculated.Results In this study,21 kinds of bacteria and mycoplasma were detected in the tracheas of these mongolian gerbils.The positive rates of different bacterial and mycoplasma infection were varying greatly,between 1.5%(1/65) to 64.6%(42/65),among which,some bacteria were pathogenic to mice and rats.Conclusion This study provides reference information for the development of microbiological standards of Mongolian gerbils.