Asian Herpetological Research Instructions for Authors

Asian Herpetological Research （AHR）, an international journal, is published quarterly by the Chengdu Institute of Biology, Chinese Academy of Sciences （CIB）, and Science Press in cooperation with the Asiatic Herpetological Research Society （AHRS）. The scope of the journal includes all contemporary herpetological research areas including taxonomy, morphology, phylogeny, systematics, evolution, zoogeography, physiology, ecology, toxicology and conservation biology of amphibians and reptiles.     

Introduction to Asian Herpetological Research

正Asian Herpetological Research(AHR),an international English language journal,is published quarterly by the Chengdu Institute of Biology(CIB).Chinese Academy of Sciences and the Science Press of China,cooperated with the Asiatic Herpetological Research Society(AHRS),with its registered numbers:CN 51-1735/Q and ISSN 2095-0357,and post distribution code:62-218.AHR has an international Editorial Board consisting of many top herpetologists from different countries in the world.The journal's website can be found at:http://www.ahr-journal.com.     

Introduction to Asian Herpetological Research

Asian Herpetological Research （AHR）, an international English language joumal, is published quarterly by the Chengdu Institute of Biology （CIB）, Chinese Academy of Sciences and the Science Press of China, cooperated with the Asiatic Herpetological Research Society （AHRS）, with its registered numbers： CN 51-1735/Q and I SSN 2095-0357, and post distribution code： 62-218. AHR has an intemational Editorial Board consisting of many top herpetologists from different countries in the world. The joumal＇s website can be found at： http：//www.ahr-joumal.com.     

Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis

This study reports the initial separation of phospholipase C-alpha from porcine retina using non-denaturing two-dimensional gel electrophoresis (2-DE). Detection was by negative staining and then its hydrolytic activity was estimated using alpha-naphthyl acetate in a 2-DE gel. A spot of phospholipase C-alpha separated by 2-DE was excised. It was then electrophoretically transferred to an anion-exchange solid phase column after 40 mg, equal to dry weight of the solid resin from the cartridge (Accell Plus QMA, Waters Corporation), was packed into a disposable 1 ml syringe to make an anion-exchange solid phase column. Phosphatidylcholine was hydrolyzed in the anion-exchange solid phase column containing phospholipase C-alpha. The results indicated that a column with hydrolytic activity could be produced once lipases separated by non-denaturing 2-DE were transferred to the solid phase column.     

Asian Herpetological Research Instructions for Authors

Asian Herpetological Research （AHR）, an international journal, is published quarterly by the Chengdu Institute of Biology, Chinese Academy of Sciences （CIB）, and Science Press in cooperation with the Asiatic Herpetological Research Society （AHRS）. The scope of the journal includes all contemporary herpetological research areas including taxonomy, morphology, phylogeny, systematics, evolution, zoogeography, physiology, ecology, toxicology and conservation biology of amphibians and reptiles.     

Asian Herpetological Research Instructions for Authors

Asian Herpetological Research （AHR）, an international journal, is published quarterly by the Chengdu Institute of Biology, Chinese Academy of Sciences （CIB）, and Science Press in cooperation with the Asiatic Herpetological Research Society （AHRS）. The scope of the journal includes all contemporary herpetological research areas including taxonomy, morphology, phylogeny, systematics, evolution, zoogeography, physiology, ecology, toxicology and conservation biology of amphibians and reptiles.     

Introduction to Asian Herpetological Research

Asian Herpetological Research （AHR）, an international English language journal, is published quarterly by the Chengdu Institute of Biology （CIB）, Chinese Academy of Sciences and the Science Press of China, cooperated with the Asiatic Herpetological Research Society （AHRS）, with its registered numbers： CN 51-1735/Q and ISSN 2095-0357, and post distribution code： 62-218. AHR has an international Editorial Board consisting of many top herpetologists from different countries in the world. The journal＇s website can be found at： http：//www.ahr-journal.com.