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The Involvement of Corin in the Progression of Diabetic Erectile Dysfunction in a Rat Model by Down‐Regulating ANP /NO/cGMP Signal Pathway 下载免费PDF全文
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Yu S Xi Z Hai-Yan C Ya-Li C Shao-Hu X Chuan-Sen Z Xiang-Qun Y Jin-Ping G Hai-Yan L Lei D 《Journal of cellular biochemistry》2012,113(8):2671-2678
High glucose-induced proliferation of vascular smooth muscle cells (VSMCs) plays an important role in the development of diabetic vascular diseases. However, molecular mediators responding for the proliferation of VSMCs remain to be determined. In this study, VSMCs were isolated from the rat thoracic aorta, and two cell models with Irf-1 knockdown and overexpression were established by transfecting cells with pGCsi-FU-Irf-1 and pGC-FU-Irf-1, respectively. Subsequently, high glucose was added to cells to induce proliferation. Proliferation assays were performed to see whether Irf-1 was involved in high glucose-induced proliferation of VSMCs. In addition, the expression of Irf-1 was detected in VSMCs stimulated with high glucose and the thoracic aorta of diabetic rats to confirm the relationship between Irf-1 expression and the proliferation of hyperglycemia-dependent VSMCs. The results showed that Irf-1 expression was significantly higher in the thoracic aorta of diabetic rats and VSMCs stimulated with high glucose than that in nondiabetic rats and untreated cells. Overexpression of Irf-1 accelerated the proliferation of VSMCs, and down-regulation of Irf-1 expression significantly depressed the proliferative ability of VSMCs under high-glucose conditions, indicating that Irf-1 was a positive regulator for high glucose-induced proliferation of VSMCs. It could be presumed that Irf-1 is associated with the accelerated proliferation of VSMCs in diabetic vascular diseases and may prove to be a potential target gene for disease treatment. 相似文献
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《Redox report : communications in free radical research》2013,18(5):223-230
AbstractAdvanced diabetic nephropathy is characterized by abnormal synthesis of extracellular matrix (ECM) proteins, such as collagen I (COL I). The present experiments were designed to test the hypothesis that the presence of abnormal ECM proteins may be responsible for increased generation of reactive oxygen species (ROS) that are thought to have an important role in the pathogenesis of diabetic nephropathy. SV40 MES 13 murine mesangial cells were plated on COL I or collagen IV (COL IV) for 3 h at 5.5 or 25 mM D-glucose concentration. Increased intracellular ROS generation and reduced intracellular nitric oxide (NO) production was measured in cells attached to COL I compared with cells attached to COL IV. Treatment with Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of NO synthase, reduced this difference in ROS generation between cells attached to either COL I or IV. The results using antibodies against integrins also indicated that an α2 integrin-mediated pathway was involved in the different response in ROS generation caused by ECM proteins. These results suggest that contact between altered ECM proteins that are present in advanced diabetic nephropathy and mesangial cells has the potential to increase intracellular oxidative stress, leading to progressive glomerular damage. 相似文献
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Human Wharton's Jelly Stem Cells and Its Conditioned Medium Enhance Healing of Excisional and Diabetic Wounds 下载免费PDF全文
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A Nanoscaffold Impregnated With Human Wharton's Jelly Stem Cells or Its Secretions Improves Healing of Wounds 下载免费PDF全文
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Src Homology 2 Domain‐Containing Inositol 5′‐Phosphatase Ameliorates High Glucose‐Induced Extracellular Matrix Deposition via the Phosphatidylinositol 3‐Kinase/Protein Kinase B Pathway in Renal Tubular Epithelial Cells 下载免费PDF全文
Fan Li Lisha Li Jun Hao Shuxia Liu Huijun Duan 《Journal of cellular biochemistry》2017,118(8):2271-2284