韩国1998-2001行政改革的基本经验

此次韩国行政改革在政府机构、管理制度、行政文化等相关层面施行了平衡到位相互协调的改革举措 ;改革的切入点是以职能转变为中心的政府机构重组、开放型人事制度的推开、高级公务员年薪制的试行、责任运营制度的建立、绩效管理制度的试验 ,顾客宪章制度的引进 ,以及部分公共管理事务外部委托制的探索 ;其基本经验是 ,机构改革的效度有赖于行政系统内相关要素的全面优化 ,行政改革的效度则有赖于整体改革环境的优化 ,民间专家参与是促进政府改革的重要资源 ,政府间行政架构模式决定了地方机构改革的成效。     

人肺癌中TNF-α基因多态性分布的意义及其对细胞凋亡的影响

目的:观察TNF-α基因启动子区-308位核苷酸多态性基因型在肺癌和对照人群中的分布,以及这种多态性对该基因表达和细胞凋亡的影响。方法:PER-RFLP分析112例肺癌和99例正常人的TNF-α基因启动子区-308位核苷酸多态性,TNF-α-308*G等位基因记做TNF-α1,TNF-α-308*A等位基因记做TNF- α2。免疫组织化学方法检测肺癌组织中TNF-α的表达,比较不同基因型肺癌病例的标本之间TNF-α基因表达的差异性。TUNEL法检测肺癌组织中细胞凋亡,并对比分析TNF-α-308位核苷酸多态性及其对肺癌组织中TNF -α表达和不同类型细胞凋亡的影响。结果:肺癌组与对照组的基因型分布为TNF-α1/1(52．7％、46．5％)。 TNF-α1/2(35．7％、48．5％),TNF-α2／2(11．6％、5．1％);等位基因频率为TNF-α1(70．5％、70．7％),TNF- α2(29．5％、29．3％),组间没有显著性差异,P>0．05。两种基因型个体在TNF-α基因表达上,TNF-α2/2基因型个体高于TNF-α1／1基因型的个体,两者差异显著,P<0．01。TNF-α表达的高低与癌细胞凋亡有比较明确的关系,TNF-α表达高的病例,肺癌细胞凋亡也多,与表达低的病例之间有明显差异,P<0．01。TNF-α表达的高低与间质细胞凋亡之间没有明确的关系。间质细胞平均凋亡指数在高、低分化的肺癌中分别为3．4±0．4和 3．1±0．5,组间没有显著性差异,P>0．015;癌细胞的平均凋亡指数在高、低分化的肺癌中分别为2．4±0．3和 1．7±0．4,两组间有显著性差异,P<0．05,,结论:TNF-α-308位核苷酸的多态性基因型在肺癌和正常人中的分布之间没有明显差别,这种多态性可能不是肺癌易感的遗传因素。但这种多态性与该基因的表达有关。TNF-α高表达肺癌细胞凋亡较多,间质细胞凋亡与TNF-α表达的高低变化之间没有明确的关系。在高分化的肺癌中, TNF-α表达较高,癌细胞凋亡也多,TNF-α-308位核苷酸的多态性可能与了肺癌的分化有关。     

中共八大对社会主义体制改革的探索

中共八大从政治体制和经济体制两方面对我国社会主义体制改革进行了探索,文章认为这些有益的探索初步指明了社会主义体制改革的方向,是中国共产党探索中国特色社会主义建设道路的伟大起点。     

Does online voting change the outcome? Evidence from a multi-mode public policy referendum

Do online and offline voters differ in terms of policy preferences? The growth of Internet voting in recent years has opened up new channels of participation. Whether or not political outcomes change as a consequence of new modes of voting is an open question. Here we analyze all the votes cast both offline (n = 5.7 million) and online (n = 1.3 million) and compare the actual vote choices in a public policy referendum, the world's largest participatory budgeting process, in Rio Grande do Sul in June 2014. In addition to examining aggregate outcomes, we also conducted two surveys to better understand the demographic profiles of who chooses to vote online and offline. We find that policy preferences of online and offline voters are no different, even though our data suggest important demographic differences between offline and online voters.     

猪水泡病病毒结构蛋白编码区核苷酸的序列测定与分析

以猪水泡病病毒 (Swinevesiculardiseasevirus ,SVDV)HK’70为材料 ,用特异性引物扩增出P1区的 2个重叠目的片段 ,连接于pMD 18 T载体 ,转化JM 10 9感受态细胞。经重组质粒的双酶切 ,PCR鉴定后测序。序列分析表明 ,HK’70P1区核苷酸组成中A含量较高 ,G C含量较低 ,且A G、C T转换率较高。P1序列同源性分析表明 ,SVDVHK’70与J1’73、H/3’76、SVDV(Seechurn等测株 )、NET/1/92的核苷酸序列同源性分别为 97.8%、98.1%、97.6 %和 89.3% ;相应地 ,推导的氨基酸序列同源性分别为 98.8%、98.7%、98.8%和 96 .5 %。     

应税事实依据经济实质认定之稽征规则——基于台湾地区“税捐稽征法”第12条之1的研究

台湾地区税捐稽征法第12条之1课税构成要件之认定及明订举证之责任,将税捐稽征中已实行多年的实质课税原则条文化。当避税安排的法律形式与其经济实质不相符时,稽征机关认定应税事实应以实质经济事实关系及其所生实质经济利益之归属与享有为依据。由量能课税原则导出的实质课税原则,重经济实质甚于法律形式,是实现税捐正义的实质性手段,并不违背税收法定原则。稽征机关实质课税的自由裁量权理应谦抑行使,其以税捐负担的公平分配为原则,以不损害纳税人的合法权益为底限。稽征机关依据经济实质认定应税事实,应依法承担举证责任,纳税人依法应尽协力义务,否则,稽征机关有权推定应税事实。大陆《税收征管法》亦应增订实质课税原则。     

P物质在过敏性休克家兔胃肠道内的表达

目的观察P物质在过敏性休克家兔胃肠道组织中随时间变化的规律,探讨其在过敏性休克中的作用。方法 30只家兔随机分为实验组(24只)和对照组(6只)。实验组采用多人混合血清致敏家兔,建立过敏性休克模型。休克不同时间段(猝死即刻、休克0.5h、休克1h、休克2h)提取家兔胃、小肠、圆小囊。冰冻切片,应用免疫组化(IHC)SP法和原位杂交(ISH)方法进行P物质染色,BI-2000计算机图像分析系统进行图像分析,计算阳性积分光密度值(IOD),用SPSS11.5统计软件进行方差分析,SNK检验。结果与对照组相比,实验组各小组胃、肠、圆小囊P物质蛋白和mRNA表达明显增加,且随着休克时间的变化逐渐减弱(P0.001)。IHC方差分析结果为:胃组织F=198.008,肠组织F=56.796,圆小囊组织F=67.231;ISH统计学分析方差分析结果为:胃组织F=67.506,肠组织F=72.117,圆小囊组织F=216.798。结论家兔胃肠道P物质在过敏性休克后一段时间内(2h)均表达增高,随休克时间的延长逐渐下降。     

Patterns of Middle School Adjustment and Ninth Grade Adaptation of Rural African American Youth: Grades and Substance Use

The transition to high school has been identified as a potentially difficult time in adolescents’ lives. Reductions in both academic and social functioning often accompany this transition. While these effects have been documented in urban youth, the move to high school has not been extensively studied in rural minority youth. Toward that end, the academic grades and substance use in ninth grade of 447 (184 male and 263 female) African-American adolescents from two rural counties in a state in the deep South were examined in relation to configurations of adaptation from sixth through eighth grade. Results indicate that individual with consistently positive patterns across middle school had higher grades and lower rates of substance use compared to individuals with persistent difficulties or those that transitioned to problem behavior. Many individuals who improved in their patterns of adaptation had relatively high grades, but also rather high rates of substance use in the ninth grade. David B. Estell is an assistant professor of educational psychology at Indiana University Bloomington. He received his PhD in Developmental Psychology from the University of North Carolina at Chapel Hill. His major research interests include peer relations and the development of aggression. Thomas W. Farmer is an associate professor of education at Pennsylvania State University and director of the National Research Center on Rural Education Support. He received his PhD in Special Education from University of North Carolina at Chapel Hill. His major research interests include peer relations and the development of aggression in students with and without special needs. Matthew J. Irvin is a research scientist at the Center for Developmental Science at the University of North Carolina at Chapel Hill. He received his PhD in Educational Psychology from the University of North Carolina at Chapel Hill. His major research interests include resilience and student engagement. Jana H. Thompson is a research associate at the Center for Developmental Science at the University of North Carolina at Chapel Hill. Her research interests include peer social relations and developmental transitions into early adolescence. Bryan C. Hutchins is a research assistant at the Center for Developmental Science at the University of North Carolina at Chapel Hill. He is also a graduate student in the Educational Psychology, Measurement, and Evaluation Program at UNC. His research interests include child and adolescent social development and school based emotional and behavioral interventions and prevention programs. Erin M. McDonough is currently a doctoral candidate in School Psychology at the University of North Carolina at Chapel Hill. She received her Bachelor of Arts degree in Psychology from Emory University. As a research assistant at the Center for Developmental Science, she has been able to explore her interests in student achievement as well as rural education. Another major research interest of hers is school-based mental health.     

做中国特色社会主义共同理想的坚定信仰者

党的十七大重申坚定马克思主义理论信仰、坚定中国特色社会主义共同理想，有着特殊的现实针对性。坚定中国特色社会主义理想信念，应当成为当代中国共产党人的自觉追求。坚定中国特色社会主义理想信念．既要坚持科学社会主义的基本原则，叉要结合我国实际赋予其鲜明的中国特色，同时，必须站在新的历史起点上，努力使中国特色社会主义道路越走越宽广。     

中共三代领导集体行政管理思想比较

中共三代领导集体的行政管理思想在指导思想、社会价值取向以及领导主体等方面存在着相同点,但他们在行政治理理念、对民主和法治的认识和重视程度等方面存在着显著的差异。