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1.
XIII. Yeast sequencing reports. Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae
We have cloned NES24 using a temperature-sensitive nes24-1 mutant as a host and sequenced a 3162 bp XhoI-EcoRI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24-1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature-sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052. 相似文献
2.
We have isolated a mutant which exhibits partial constitutivity for a -specific gene expression in α cells. The wild-type gene was cloned and demonstrated to be allelic to the STE13 gene, which encodes the dipeptidyl aminopeptidase involved in processing of the α-factor prepropheromone. Thus, the mating defect of the ste13 mutations in α cells may result both from the production of incompletely processed α-factor and from the increased expression of a -specific genes. The STE13 open reading frame of 931 amino acids contains a putative membrane-spanning segment near its amino terminus and is 31% identical to a second yeast dipeptidyl aminopeptidase (DAP2). A null mutant of STE13 has been constructed: it is viable and sporulation-proficient. The sequence has been deposited in the GenBank data library under Accession Number L21944. 相似文献
3.
Michael E. Cusick 《Yeast (Chichester, England)》1994,10(9):1251-1256
A 3·2 kb EcoRI fragment of yeast Saccharomyces cerevisiae was entirely sequenced. Two new open reading frames were identified. The first is extremely hydrophobic, and would likely be an integral membrane protein. It has significant similarity to only one reported gene, a gene of unknown function from Drosophila melanogaster. The second ORF is asparagine-rich and very serine-rich, with a remarkable stretch of nearly 26 consecutive asparagine residues comprised of the same codon. It has no significant similarity to any reported gene. The fragment maps to chromosome II on the left arm between the CDC27 and ILS1 loci. The nucleotide sequence reported in this paper has been deposited in the GenBank database with the Accession Number M89908. 相似文献
4.
We have entirely sequenced a 10,835 bp segment of the right arm from chromosome III contained in the J11D and J11D-K3B GF clones. The segment contains seven open reading frames longer then 100 amino acids. Three of them, RVS161 (Urdaci et al., 1990; Crouzet et al., 1991), ADP1 (Purnelle et al., 1991) and PGK1 (Hitzeman et al., 1982) have been described previously. YCR10C encodes a putative membrane protein. YCR8W (encoding a putative protein kinase) and YCR14C extend inside the D10H (Skala et al., 1991) and 62B5-2D clones respectively. Four ARS elements previously reported by Palzkill et al. (1986) are located between RVS161 and YCR10C. 相似文献
5.
We have localized gene MSS51 on chromosome XII of Saccharomyces cerevisiae between the RDN1 and CDC42 loci. 'Head to head' with MSS51 is another gene, QRI5, the function of which is unknown. However, the proximity of these genes, the structure of the intergenic region and the presence of an ABF1 binding site right in the middle of this region suggest that the MSS51 and QRI5 expressions are submitted to a common regulatory process. 相似文献
6.
目的针对传统量子遗传算法无法充分利用种群中未成熟个体信息的不足,提出了基于交互更新模式的量子遗传算法(IUMQGA)并应用于几何约束求解中。方法几何约束问题的约束方程组可转化为优化模型,因此约束求解问题可以转化为优化问题。采用将遗传算法与量子理论相结合的量子遗传算法,使用双串量子染色体结构,使用交互更新策略将遗传算法中的交叉操作利用量子门变换来实现,根据不同情况采用不同的交互更新策略。这里的交互,指的是两个个体进行信息交换的过程,该过程用以产生新的个体。这不仅增加了个体间信息的交换而且充分利用了种群中未成熟个体的信息,提高了算法的收敛速度。结果通过非线性方程实例和几何约束实例测试并与其他方法比较表明,基于交互更新模式的量子遗传算法求解几何约束问题具有更好的求解精度和求解速率。双圆外公切线问题实例中,IUMQGA算法比QGA算法稳定;单圆填充问题和双圆外公切线问题实例中,通过实验求得各变量的最优值与其相应的精确值的误差在1E-2以下。结论采用交互更新模式的量子遗传算法可以很好地求解几何约束问题。 相似文献
7.
遗传算法在自动组卷中的应用 总被引:1,自引:0,他引:1
给出了利用遗传算法的全局寻优和收敛速度快的特点,解决组卷问题的方法,重点阐述了自动组卷问题中的染色体编码方法、适应度函数和遗传算子的设计。 相似文献
8.
9.
Wenya Wang Xi Zheng Anca Azoitei Axel John Friedemann Zengerling Felix Wezel Christian Bolenz Cagatay Günes 《International journal of molecular sciences》2022,23(22)
TKS5 promotes invasion and migration through the formation of invadopodia in some tumour cells, and it also has an important physiological function in cell migration through podosome formation in various nontumour cells. To date, the role of TKS5 in urothelial cells, and its potential role in BC initiation and progression, has not yet been addressed. Moreover, the contribution of TKS5 to ploidy control and chromosome stability has not been reported in previous studies. Therefore, in the present study, we wished to address the following questions: (i) Is TKS5 involved in the ploidy control of urothelial cells? (ii) What is the mechanism that leads to aneuploidy in response to TKS5 knockdown? (iii) Is TKS5 an oncogene or tumour-suppressor gene in the context of BC? (iv) Does TKS5 affect the proliferation, migration and invasion of BC cells? We assessed the gene and protein expressions via qPCR and Western blot analyses in a set of nontumour cell strains (Y235T, HBLAK and UROtsa) and a set of BC cell lines (RT4, T24, UMUC3 and J82). Following the shRNA knockdown in the TKS5-proficient cells and the ectopic TKS5 expression in the cell lines with low/absent TKS5 expression, we performed functional experiments, such as metaphase, invadopodia and gelatine degradation assays. Moreover, we determined the invasion and migration abilities of these genetically modified cells by using the Boyden chamber and wound-healing assays. The TKS5 expression was lower in the bladder cancer cell lines with higher invasive capacities (T24, UMUC3 and J82) compared to the nontumour cell lines from human ureter (Y235T, HBLAK and UROtsa) and the noninvasive BC cell line RT4. The reduced TKS5 expression in the Y235T cells resulted in augmented aneuploidy and impaired cell division. According to the Boyden chamber and wound-healing assays, TKS5 promotes the invasion and migration of bladder cancer cells. According to the present study, TKS5 regulates the migration and invasion processes of bladder cancer (BC) cell lines and plays an important role in genome stability. 相似文献
10.
基于遗传算法的变迹叉指换能器进化设计 总被引:1,自引:0,他引:1
为简化变迹叉指换能器设计过程,提出了一种新的变迹叉指换能器的设计方法,它以叉指换能器的中心频率处的插入损耗和旁瓣电平作为寻优目标,叉指换能器的指条长度作为基因,通过选择、交叉和变异等遗传操作,自动生成叉指换能器的所有指条长度,并给出进化得到的变迹叉指换能器的频率响应.实验结果表明,提出的设计方法正确有效,所编制的进化设计软件包实用性强. 相似文献