滇白珠地上部分的化学成分研究

目的研究滇白珠(Gaultheria leucocarpa var.yunnanensis or var.crenulta)地上部分的化学成分。方法应用各种色谱技术进行分离纯化,用MS和NMR分析鉴定化合物结构。结果分离得到7个化合物,分别鉴定为①槲皮素-3-O-β-D葡萄糖醛酸苷、②山萘酚-3-O-β-D-葡萄糖醛酸苷、③龙胆酸甲酯、④水杨酸甲酯、⑤白珠树苷、⑥滇白珠素A、⑦滇白珠素B。结论化合物1～3是首次从该植物中分离得到。     

HPLC测定民族药滇白珠抗炎镇痛活性部位(ARF)中4种活性成分的含量

目的:建立高效液相色谱法(HPLC)测定民族药滇白珠抗炎镇痛活性部位(ARF)中4种活性成分methyl benzoate-2-O-β-D-xylopyranosyl (1-2)[O-β-D-xylopyranosyl (1-6)]-O-β-D-glucopyranoside (MSTG-A),methyl benzoate-2-O-β-Dglucopyranosyl(1-2)[O-β-D-xylopyranosyl(1-6)]-O-β-D-glucopyranoside(MSTG-B),gaultherin及绿原酸的含量测定方法,为滇白珠药材的深入研究及其质量控制提供方法依据,并为后期滇白珠的制剂与临床应用奠定基础。方法:采用COSMOSIL 5C18-PAQ色谱柱(4. 6 mm×250 mm,5μm),以甲醇-0. 2%冰乙酸水溶液为流动相梯度洗脱,柱温25℃,流速1 m L·min~(-1),检测波长294 nm。结果:MSTG-A,MSTG-B,gaultherin,绿原酸分别在0. 014 06～0. 450 00,0. 007 81～0. 250 00,0. 003 13～0. 100 00,0. 000 94～0. 030 00 g·L~(-1)线性关系良好(r≥0. 999 7);方法精密度、重复性、稳定性良好;平均加样回收率分别在100. 81%,99. 19%,96. 12%,102. 56%,其RSD分别为1. 4%,0. 7%,0. 7%,2. 4%。滇白珠ARF部位中MSTG-B,MSTG-A,gaultherin,绿原酸质量分数分别为23. 608,41. 973,8. 282,2. 673 mg·g~(-1)。结论:该方法操作简便、准确、重复性好,适用于滇白珠抗炎镇痛活性部位中4种活性成分的含量测定,为滇白珠药材的深入研究、质量控制与综合评价提供参考依据,并为后期滇白珠药材的制剂与临床应用奠定基础。     

Synthesis and anti-nociceptive and anti-inflammatory effects of gaultherin and its analogs

The synthesis of gaultherin (1) and its analogs was carried out to provide 11 glycosides under phase-transfer catalytic conditions. The activities of all synthesized compounds were evaluated by nitric oxide production inhibitory assay in vitro. Methyl 2-O-(4-O-β-d-galactopyranosyl)-β-d-glucopyranosylbenzoate (5f) showed significantly anti-nociceptive and anti-inflammatory effects by the evaluation in vivo. Structure–activity relationships within these compounds were discussed.