GC法测定金银三七胶囊中的冰片的含量

目的：建立金银三七胶囊中冰片的含量测定方法。方法：采用GC法，色谱柱为10％PEG-20M；FID氢火焰检测器：柱温为115℃。结果：冰片在0．02559-0．5118μg范围内线性良好。加样回收率为99．9％，RSD％为1．94％（n=9）。结论：方法简便，可行，重现性良好，结果准确，可加强金银三七胶囊的质量控制。     

Fluorimetric assay of rufloxacin in serum and in pharmaceutical formulations

A simple and rapid fluorimetric method for the determination of 9-fluoro-10-[N-(4′-methyl)piperazinyl]-7-oxo-2,3-dihydro-7H-pyrido[1,2,3-d,e][1–4]benzothyazin-6-carboxylic acid hydrochloride (MF 934), in serum and in pharmaceutical formulations, has been developed based on its strong fluorescence, in 0.1 N H₂SO₄, at 526 nm (excitation wavelength at 340 nm). The procedure which involves the direct dilution of the sample requires only a few minutes and the sample volume is only 20–100 μl of serum, depending on the drug concentration. Tedious sample preparation procedures such as extraction, deproteinization, or centrifugation are not necessary. The minimum concentration that can be detected is 0.3 ng ml⁻¹, the standard curve in 0.1 N H₂SO₄ was found to be linear from 0.005 to 1.5 μg ml⁻¹ and from 0.01 to 0.07 g in plasma after dilution with 0.1 N H₂SO₄.     

灵芝菌合剂中灵芝多糖含量的测定

目的：探讨灵芝菌合剂中灵芝多糖含量的检测方法。方法：采用分光光度法，以无水葡萄糖为对照品，检测波长为490nm。结果：此方法在0～120μg范围内线性关系良好，灵芝多糖的平均回收率为99．09%。RSB为1．14％。结论：本法简便快捷，准确度高，重现性好，可作为本制剂的质控方法。     

蛋黄磷脂中磷脂酰乙醇胺的薄层扫描测定

将超临界ＣＯ２萃取法得到的蛋黄粉中的磷脂经薄层色谱分离，采用双波长薄层扫描仪对其磷脂酰乙醇胺进行测定。平均回收率为９７．６７％，ＲＳＤ为２．３１％。     

甘草黄酮的提取分离和含量测定

目的：充分利用甘草资源。方法：从提取过甘草酸的废渣中提取甘草黄酮并进行含量测定。结果：用碱酸处理和用大孔树脂处理黄酮含量高，收率也高。结论：从甘草废渣中提取甘草黄酮具有较好的社会效益和经济效益     

Capillary gas chromatographic assay of camphor and m-cresol in dermatological creams

Camphor and m-cresol mixtures are used in antiseptic and anti-itching creams. No compendial method exists for these preparations. This paper reports a capillary gas chromatographic method using FID detection with 2,6-di-tert-butyl-4-methylphenol as internal standard on a 30 m×0.32 mm Supelcowax®-10 column (0.25 μm film) with helium as carrier gas. Ramped temperature programming was applied. The method allows simultaneous quantitation of camphor and m-cresol in the presence of o- and p-cresols, calamine and zinc oxide. Overall percent recoveries (±SD, n=9) of camphor, o-, p- and m-cresol from spiked placebo creams, at a labeled amount of 10 (w/w)% were 96.9±0.6, 98.2±0.6, 99.2±0.5 and 101.0±0.9%, respectively, and at a labeled amount of 1% were 96.7±0.6, 97.8±0.9, 97.8±0.6, and 100.3±1.0%, respectively. The recovery studies were carried out at ±30% of the labeled amounts. Linear peak area or height ratios were obtained (r>0.999) for camphor, o-, p- and m-cresol covering a concentration range of 10–200% of the labeled amount. Linearity (r>0.999) was also obtained for m-cresol when the relative concentration of o- and p-cresol was varied from 5 to 100% of the m-cresol concentration. The resolution between the ‘critical pair’ of p- and m-cresol was ≥1.1. The limit of quantitation was 23 pg for m-cresol and 9.3 pg for camphor using an injection split of 1:50. The repeatability (%RSD) for all compounds were <2% for peak area and <1.4% for peak height ratios. System suitability and robustness of the method were established. The method was successively applied to the assay of available commercial products and allows assay of camphor and the three cresol isomers.     

复方栀子冲剂栀子甙薄层扫描测定

应用薄层扫描法测定了复方栀子冲剂中栀子甙的含量，对实验条件，以正交试验法进行优选，确定了最佳方案，排除了干扰，得到了满意的效果。     

管碟法测定Nisin效价

对管碟法测定Nisin效价的条件进行了研究,考察了几个参数的影响,得出了Nisin测定的最佳条件:90mm培养皿中培养基加量为15mL,Na2HPO4·12H2O质量浓度1g/dL,菌悬液浓度109CFU/mL,琼脂质量浓度1g/dL,培养基pH值7.0,牛津杯中样品加液量100μL.在此条件下,Nisin效价在5～100IU/mL,其对数值与抑菌圈直径有较好的线性关系.     

长春西汀片中阿朴长春胺酸乙酯含量的紫外分光光度法测定

采用紫外分光光度法测定长春丁汀片的含量。长春西汀片在２７４ｍｍ波长处有最大吸收，方法线性范围是１０－２０μｇ／ｍｌ，回归方程Ｙ＝３０．０９２Ｘ＋０．０５３３，ｒ＝０．９９９９，平均回收率为１０１．３８％（ｎ＝５，ＲＳＤ＝０．７１％）。