Cytochemical staining characteristics of lymph nodes from normal and lymphoma-affected dogs

Frozen sections and imprint smears were used to evaluate the presence and pattern of cytochemical staining reactions in the B- and T-cell regions of lymph nodes from normal dogs and dogs with lymphoma. Staining procedures evaluated included peroxidase (PER), Sudan black B (SBB), naphthol AS-D chloroacetate esterase (CAE), alpha-naphthyl butyrate esterase (NBE), acid phosphatase (ACP), and leukocyte alkaline phosphatase (LAP). In normal lymph nodes, macrophages and some lymphocytes within the interfollicular (T-cell) region and medulla stained positive with ACP and NBE. Smaller numbers of macrophages also occurred sporadically within the germinal follicles. Cells positive for PER, SBB, and CAE were scattered infrequently throughout all regions of the normal lymph node, consistent with granulocytes and mast cells. The LAP stained cells were predominantly and prominently located within the mantle zone of secondary follicles and to a much lesser extent within the germinal centers, compatible with B-cell lymphocytes derived from follicular center cells. Of the 12 dogs with lymphoma, 7 cases (4 immunoblastic, 2 large noncleaved, 1 small noncleaved) stained diffusely positive with LAP, 4 cases (all lymphoblastic) had numerous focally positive lymphocytes using ACP and NBE, and 1 case (immunoblastic) did not stain positive with any of the cytochemical reactions. Cytochemical staining of canine lymph nodes with NBE, ACP, and LAP proved useful in distinguishing between B- or T-cell regions and detecting different cell types of canine lymphoma.     

自动检索最小独立闭合环的算法

控制测量数据内业平差处理时,对于多边形控制网而言,以最小独立闭合环为单位进行平差。基于站点的角度外业观测数据,提出最小独立闭合环的计算机自动快速检索算法。     

农杆菌介导的pac1基因转化大豆研究

选用高感大豆花叶病毒病而再生能力较强的大豆品种和品系作为受体,对丛生芽诱导培养基、侵染菌液的制备以及根的诱导方法进行了优化。采用优化后的遗传转化体系以农杆菌介导法将pac1导入大豆,MSB培养基中附加2 mg/L6-BA+0.2 mg/LIBA进行丛生芽诱导,液体YEB重悬活化菌体后侵染,MSB培养基中蔗糖含量15%+2 mg/LIBA+0.2 mg/L6-BA进行生根诱导。共获得8株PCR检测呈阳性植株,经SouthernBlot检测,证明pac1基因已整合到大豆基因组中。转基因植株对病毒的抗性评价正在进行中。     

毛竹材径向篾抗拉和抗弯性能研究

[目的]目前对于径向竹篾的相关研究较少,且未有竹节对径向竹篾性能影响方面的研究.研究随高度变化径向竹篾的结构特征、抗弯和抗拉性能变化规律,以及竹节对其力学性能的影响,可为径向竹篾在竹木复合集装箱底板应用中的结构设计和工艺优化提供理论依据以及技术参考.[方法]采用4年生不同高度毛竹制备的径向竹篾,分析不同高度等级的径向竹...     

Sentinel lymph node mapping with computed tomography lymphography for mast cell tumours and a comparison between regional and sentinel lymph node histological status: Sixty-two cases

It is known that the regional lymph node (RLN) may not correspond to the sentinel lymph node (SLN) (the first lymph node draining the tumour), and many diagnostic techniques have recently been aimed at its detection. Although lymphoscintigraphy is the gold standard in both human and veterinary medicine for SLN mapping, it is relatively unavailable in veterinary medicine due to costs and difficult management of the radiotracer. This prospective study evaluated, as a first aim, the feasibility and sensitivity of the computed tomography lymphography (CTL) in detecting the SLN in 62 mast cell tumours (MCTs). The second aim was to evaluate the accuracy of the CTL in identifying the most representative lymph node of the patient's lymphatic status; the histological status of the SNL was compared with that of the RLN, to see in how many cases the patient's stage would have changed according to the RLN. When the RLN turned out to be also the SLN it was decided to excise, as a control LN, the one localised in the neighbourhood of the MCT (neighbouring lymph node; NLN). The detection rate was 90%, with failure of SLN identification in six cases. In 18 (32%) of 56 MCTs with a diagnostic CTL, the SLN did not correspond to the RLN. Forty-five MCTs were surgically removed, together with their corresponding SLN and RLN/NLN. Since the clinical stage of the patient would have changed in only 7% of cases, CTL is a reliable method of detecting the SLN and, for staging purposes, there is no need to remove other LNs.     

Mesenteric lymph drainage alleviates spleen injury in hemorrhagic shock rats

AIM：To observe the effects of post-shock mesenteric lymph (PSML) drainage on histopathology, apoptosis, cell cycle and proliferation of the spleen in rats with hemorrhagic shock. METHODS：Eighteen Wistar rats were randomly divided into sham, shock and shock+drainage groups (n=6 in each group). The hemorrhagic shock model was established in the shock and shock+drainage groups. Fluid resuscitation for 30 min was performed 1.5 h after hypotension, and PSML was drained in the rats in shock+drainage group from 1 h after hypotension to 3 h after resuscitation finished. The fixed spleen tissue was harvested from each rat for histological observation with HE staining. The apoptosis of splenocytes was observed by Hoechst 33258 staining. The expression of Bcl-2 and Bax proteins was detected by immunohistochemical staining. The cell cycle and the expression of p53 protein were measured by flow cytometry, and the proliferation index (PI) was calculated. RESULTS：Compared with sham group, splenic tissue injury appeared in the shocked rats. The apoptotic cells and the expression of Bax and p53 in shock group were increased, while Bcl-2 expression was decreased. The percentage of G2/M cells in shock group was decreased. Compared with shock group, the splenic tissue damage in shock+drainage group was significantly attenuated. Moreover, the number of apoptotic cells, the percentage of G0/G1 cells, and the expression of Bax and p53 were obviously decreased, and the G2/M cells, Bcl-2 protein expression and PI were significantly increased in shock+drainage group. CONCLUSION: PSML drainage alleviates splenic injury in hemorrhagic shock rats, which may be related to reducing the apoptosis of splenocytes.     

非洲菊离体快繁条件的优化

以非洲菊无菌苗带节茎段为外植体,比较各种不同激素组合、浓度大小对非洲菊芽苗的诱导、增殖和生根的影响。结果表明,以0.8-1.2 cm的非洲菊带节茎段为外植体,可以不通过愈伤组织途径,直接诱导出芽。非洲菊带节茎段诱导芽苗的最佳培养基为MS+6-BA3.0 mg.L-1+NAA0.1 mg.L-1。较高浓度的6-BA有利于增殖系数的提高,但容易出现玻璃化现象,高浓度的NAA会使芽苗叶色退绿,故最适合的增殖培养基为MS+6-BA2.0 mg.L-1+NAA0.3 mg.L-1。非洲菊芽苗生根的浓度范围较宽,最佳生根培养基为1/2MS+NAA0.2 mg.L-1。     

水产养殖水质环境无线监测系统设计与实现

针对目前水产养殖环境监测手段及监测设备基础薄弱、相对落后的现状,应用无线传感技术、嵌入式计算技术、微机电技术(MEMS)、分布式信息处理技术及无线通信网络技术于一体的无线传感器网络,为水产养殖环境提供数字化、网络化、智能化的实时动态监测系统。该系统不仅能够对水产养殖环境的主要检测指标(水温、PH值、溶氧量、浊度、氨氮等)进行实时监测,还能够对检测指标进行数据融合和挖掘,以建立水产养殖环境检测指标历史数据库,实现监测数据的本地或远程、实时、动态显示和分析,为水产养殖过程中有效提高水资源利用率、改善养殖环境质量、降低污染物的排放等提供了一种重要技术手段和科学依据。     

基于无线传感网络的农业无人机航线控制系统

无人机是一个由飞行器、控制站、通讯设备和其它部件形成的系统,在农业领域主要应用于农药喷洒、信息监测和农业保险勘察。农业无人机在飞行过程中的实际航线与规划航路之间会存在偏差,不仅降低了作业质量,还会影响作业效率。无线传感网络是一种与无人机紧密结合的技术,可以用于对无人机的航线进行控制。为此,基于无线传感网络,设计了无人机的航线控制系统。该系统由无人机平台、传感节点、汇聚节点和控制中心4部分组成,对航线的控制通过二维坐标系跟随算法完成。试验结果表明:无线传感网络对直线和曲线航线的跟踪更加稳定,具有较高的航线控制精确度。     

基于 ZigBee 技术的农田监测系统设计

当前无线传感器网络技术逐渐成熟,促进了智能农业和精准农业的发展。为获得实地、大范围和实时的农田信息,提出了一种基于ZigBee 技术的信息监测系统设计方案,包括硬件平台的设计和系统软件的开发。硬件平台以ATmega 128单片机和CC 1101射频芯片为核心,主要由数据处理单元、无线模块、传感器控制矩阵、数据存储、供电单元、模拟接口和数字接口等构成。在TinyOS 操作系统的开发平台上利用 nesC 语言实现了传感器节点和汇聚节点的软件开发。对传感器节点主要进行了传感器驱动程序设计,而汇聚节点主要进行了串口通信编程。此外,根据节点不同的工作模式,设计了节点的节能算法。该系统稳定、可靠,满足设计需求。