Explore the Application Law of Disperse Stagnation and Purge Heat with Bitter-Acrid Medicinals in Treating Endogenous Cough

内伤咳嗽以肺失宣肃、肺气上逆为基本病机。“苦辛通降助肺法”即借助药物气味的升降作用以使肺气宣降复常的治法，用于治疗内伤咳嗽可通过苦辛配伍来助肺宣降、调节气机。张之文教授为全国温病学泰斗，提出以“苦辛通降助肺法”治疗内伤咳嗽，临床效果显著。本研究以张老医案为基础，通过数据挖掘来管窥“苦辛通降助肺法”治疗内伤咳嗽的应用规律，为内伤咳嗽的治疗提供新的思路和方法。     

吹扫捕集-气相色谱-质谱联用法测定生活饮用水中的二氯甲烷

目的建立生活饮用水中二氯甲烷的吹扫捕集-气相色谱-质谱联用测定方法。方法采用吹扫捕集富集水中二氯甲烷,解吸后用气相色谱-质谱测定,选择离子扫描方式下用标准曲线法进行定量分析。结果该方法操作简便,检出限低(0.005ng/mL),回收率均大于97%,相对标准偏差小于5%。结论该方法适合生活饮用水中的二氯甲烷的测定。     

三黄胶囊的药效学研究

目的 探讨三黄胶囊的解热、通便,抗菌作用。方法 采用大鼠酵母性发热法,体外细菌培养法和小鼠肠道碳末推进实验。结果 三黄胶囊对大鼠酵母性发热有解热作用,对金黄色葡萄球菌、链球菌和痢疾杆菌有较强的抑制作用,具有明显的泻下和促进胃肠蠕动的作用。结论 三黄胶囊具有解热、抑菌及通便作用。     

吹扫捕集-GC—MS／SIM方法检测阿莫西林中的溶剂残留

目的：建立阿莫西林中甲基异丁基酮和醋酸丁酯2种挥发性有机溶剂的残留量测定方法。方法：采用用吹扫捕集浓缩仪和色谱-质谱联用（SIM）（P＆T—GC—MS／SIM）方法测定阿莫西林中甲基异丁基酮和醋酸丁酯2种挥发性有机溶剂的残留量。结果：吹扫管中2种溶剂量在0—3200ng范围内线性关系良好（r〉0．99）；吹扫管中2种溶剂量均为2400ng时，平行测定5次相对标准偏差分别为4．75％和0．66％；甲基异丁基酮和醋酸丁酯的检测限分别为2．9和4．8ng。结论：方法能满足粉状和液体药品中ng至μg级残留溶剂的测定。     

滋阴泻火中药对性早熟模型大鼠骨密度的影响

目的:研究滋阴泻火方对性早熟模型大鼠骨密度的影响。方法:采用26日龄SD大鼠36只,随机分成6组,除空白对照组外,其余5组建立性早熟模型。分别用促性腺激素拟似剂和滋阴泻火方大、中、小剂量对建立性早熟模型的大鼠进行干预。动物处死时留取胫骨,使用外周骨定量计算机断层扫描(pQCT)技术测定骨密度(包括总骨密度,骨小梁密度及骨皮质密度)。结果:性早熟模型组大鼠的骨密度(主要是骨小梁密度)较正常对照组为高(P<0.05);亮丙瑞林和中药大、中剂量能降低性早熟模型组大鼠的骨密度(P<0.05)。结论:一定剂量的滋阴泻火方能有效抑制性早熟模型大鼠骨密度的增加。     

Effect of response format on endorsement of eating disordered attitudes and behaviors

OBJECTIVE: The present study was designed to compare response rates on a standard self-report questionnaire that was nominally anonymous to an unmatched count questionnaire that allowed for true anonymity in responding. METHOD: Four hundred and fifty-four college students were asked about several topics, including attitudes towards weight and shape, dieting, and eating disordered behavior using one of two response formats; either a standard questionnaire in true-false format or an unmatched count questionnaire that did not require participants to directly answer sensitive questions. RESULTS: Both males and females had significantly different rates of endorsement between the two methods of assessment on the majority of the eating-related questions. CONCLUSION: Response format and degree of anonymity affect endorsement of eating-related thoughts and behaviors. Understanding response bias is critical to determining accurate rates of eating disordered thoughts and behaviors.     

Biological monitoring of human exposure to volatile halogenated hydrocarbons using urinalysis with capillary GC-ECD

For the risk assessment of human exposure to volatile halogenated hydrocarbons, a dynamic purge trap/on-column cryofocusing method using capillary gas chromatograph-⁶³Ni electron capture detector and thermal desorption unit was applied to analyze the free forms, metabolites of 1,1,2-trichloroethylene and 1,1,2,2-tetrachloroethylene. The urine sample was diluted with distilled water, hydrolyzed and sealed. Then the inert gas was infused to purge out free 1,1,2-trichloroethylene, free 1,1,2,2-tetrachloroethylene and trichloroethanol. These compounds were trapped to Tenax^R GC-gas trap device throughout clean up tube. Being undetectable to gas chromatograph directly, trichloroacetic acid was methyl esterificated and trapped in the manner above mentioned. The optimal incubation time to get best recovery of methyl ester was 4 hours at 60°C. The concentrations of free volatile halogenated hydrocarbons and their metabolites in urine were obtained from 5 healthy volunteers. This analytical method is expected to make the biological monitoring, more precise and convenient.     

肉苁蓉不同提取部位对便秘大鼠通便作用的影响

目的：研究肉苁蓉不同提取部位的润肠通便作用。方法：使用易蒙停建立大鼠便秘模型,计算便秘大鼠6 h的粪便粒数和重量,并观察、测定便秘大鼠的胃肠激素和肠神经递质等指标。结果：肉苁蓉不同提取部位有促进便秘大鼠排泄作用,与模型组比较,总寡糖部位组SS、AchE、SP、MTL有升高的趋势,总寡糖部位组5-HT、VIP、NT显著升高（P<0.05或P<0.01）,AQP3显著降低（P<0.01）,表明总寡糖组有明显的治疗效果,其余各组无显著性差异,提示总寡糖是肉苁蓉有效作用部位。结论：肉苁蓉的总寡糖具有较好的润畅通便作用,能有效治疗便秘,可为其开发提供科学依据。