全文获取类型
收费全文 | 779篇 |
免费 | 36篇 |
国内免费 | 31篇 |
学科分类
医药卫生 | 846篇 |
出版年
2023年 | 3篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 8篇 |
2017年 | 8篇 |
2016年 | 10篇 |
2015年 | 8篇 |
2014年 | 14篇 |
2013年 | 24篇 |
2012年 | 16篇 |
2011年 | 33篇 |
2010年 | 45篇 |
2009年 | 42篇 |
2008年 | 51篇 |
2007年 | 44篇 |
2006年 | 49篇 |
2005年 | 47篇 |
2004年 | 38篇 |
2003年 | 62篇 |
2002年 | 53篇 |
2001年 | 50篇 |
2000年 | 40篇 |
1999年 | 40篇 |
1998年 | 37篇 |
1997年 | 22篇 |
1996年 | 31篇 |
1995年 | 18篇 |
1994年 | 11篇 |
1993年 | 11篇 |
1992年 | 11篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
排序方式: 共有846条查询结果,搜索用时 0 毫秒
1.
2.
S. NAKAO J. KURATA T. ARAI M. MURAKAWA T. ADACHI M. N. AVRAMOV K. MORI O. YASUHARA I. TOOYAMA H. KIMURA 《Acta anaesthesiologica Scandinavica》1994,38(8):845-851
Recent studies have shown that proto–oncogene c–fos mRNA is induced in the central nervous system by a variety of stimuli including generalised convulsions. In this study, the expression of c–fos protein (c–Fos) following lignocaine–induced convulsions was examined and compared with that following convulsions induced by non–anesthetic convulsants, such as pentylenetetrazol, kainic acid and electroconvulsive shocks, in rat brain.
Administration of 120 mg kg-1 lignocaine by the intraperitoneal route induced generalised convulsions in all rats examined within 10 min. C–Fos was markedly induced in the piriform cortex and amygdala, and slightly induced in the neocortex and thalamus, while no c–Fos expression was observed in the hippocampus. In contrast, c–Fos expression following generalised convulsions induced by non–anaesthetic convulsants was very marked in the hippocampal region, piriform cortex and amygdala, and extended to the thalamus and neocortex.
These results contradict those of previously reported local cerebral metabolic studies using 2–deoxyglucose as a metabolic marker, and suggest that lignocaine–induced convulsions, unlike those induced by non–anaesthetic convulsants, may not cause severe sequelae (plastic changes) in the hippocampus. 相似文献
Administration of 120 mg kg
These results contradict those of previously reported local cerebral metabolic studies using 2–deoxyglucose as a metabolic marker, and suggest that lignocaine–induced convulsions, unlike those induced by non–anaesthetic convulsants, may not cause severe sequelae (plastic changes) in the hippocampus. 相似文献
3.
The external subdivision of the lateral parabrachial nucleus (LPBE) shows strong Fos-like immunoreactivity (FLI) following anorectic doses of the indirect serotonin agonist dexfenfluramine (DFEN). In an effort to determine the contribution of the LPBE to DFEN-induced anorexia, bilateral ibotenate lesions were made in the LPBE, and the effects of the lesion on DFEN-induced anorexia and FLI as well as c-June-like immunoreactivity (JLI) were examined. It was found that LPBE lesion significantly attenuated DFEN anorexia: in a 1-h food intake test following 24-h food deprivation, DFEN (2 mg/kg) suppressed food intake by 60% in intact rats but only 34% in rats with LPBE lesions. In addition to this behavioral change, LPBE lesion completely abolished DFEN-induced FLI and JLI in the lateral subdivision of the central nucleus of the amygdala (CeL) and laterodorsal subdivision of the bed nucleus of stria terminalis (BSTLD), both of which showed strong FLI and JLI in intact rats. DFEN-induced FLI and JLI in other brain regions were not affected by LPBE lesion, including the ventromedial and lateral hypothalamus, caudate-putamen, and the nucleus of the solitary tract (NST). The parallel loss of DFEN-induced anorexia and FLI/JLI following LPBE lesion raises the novel possibility that LPBE-CeL/BSTLD pathway may be involved in DFEN anorexia. 相似文献
4.
5.
Masami Yoshida Hideyasu Yokoo Kimihiro Nakahara Masaru Tomita Naoyuki Hamada Michiko Ishikawa Jyunko Hatakeyama Masatoshi Tanaka Ikuko Nagatsu 《Brain research》1997,767(2):87
Infusion of muscimol (5×10−5 M, 60 min) into the nucleus accumbens (NAC) through a dialysis membrane caused a significant increase in extracellular dopamine (DA) and its metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC). Fos-like immunoreactivity induced by intra-NAC infusion of muscimol was seen ipsilaterally in many accumbofugal target areas, but no Fos-positive neurons were seen in the vicinity of the dialysis membrane in the NAC. Sequential staining of Fos and tyrosine hydroxylase (TH) immunoreactivities revealed that a portion of A10 dopaminergic neurons were double-labelled. These results suggest that muscimol in the NAC disinhibits mesolimbic DA neuronal activity possibly through activity of the accumbofugal GABA neuron system. 相似文献
6.
The spatial and temporal pattern of expression of the protein products of immediate early genes (IEGs) c-fos, fos B, and egr-1 were mapped in medial preoptic area (MPOA) and ventral bed nucleus of stria terminalis (VBST) during maternal behavior in rats. Immunocytochemical analysis indicated significant increases in the number of cells expressing c-Fos after 2 h of pup exposure, while Fos B levels showed a delayed response, reaching maximal levels after 6 h. 相似文献
7.
Xia Zhang Gildas Le Gal La Salle Valerie Ridoux Peter H. Yu Gong Ju 《Neuroscience letters》1996,210(3):169-172
Fos oncoprotein expression is a marker of neuronal activation following seizures. Here, using this method we examined the anatomical locations of muscimol-induced absence seizures in the rat forebrain. Six hours after a systemic injection of muscimol a massive Fos immunoreactivity appeared in the olfactory system, retrosplenial cortex and paraventricular thalamic nucleus, whereas other cortical areas contained low level of Fos expression. These results provide the first functional morphological evidence suggesting that these forebrain structures with Fos expression may play an important role in the pathophysiology of muscimol-induced absence seizures. 相似文献
8.
将白细胞介素-1(IL)注入大鼠侧脑室,用Fos癌蛋白抗体免疫组化法检测了下丘脑室旁核的激活神经元:大量位于含促肾上腺皮质激素释放因子(CRF)相应区域的室旁核小细胞部神经元呈Fos免疫强阳性。Fos和CRF的免疫双染色显示了许多Fos免疫阳性神经元也呈CRF免疫阳性。此外,在IL-1注射动物中,CRF的免疫阳性显著加强,提示CRF合成增加。 相似文献
9.
研究大鼠在戊四氮导致癫痫发作早期前脑内小胶质细胞的变化及其与神经元的关系,本研究应用免疫组织化学法分别显示前脑内OX-42和Fos蛋白表达的时程变化,并用双重标记显示OX-42和Fos阳性细胞的相互关系。结果发现:在戊四氮导致大鼠癫痫发作早期(从15min到360min),前脑的小胶质细胞OX-42表达阳性,随着存活时间的变化,OX-42的阳性反应经历逐渐升高又降低的过程;Fos蛋白在神经元和小胶质细胞中有表达,也呈现逐渐升高又降低的变化;Fos在小胶质细胞表达高峰的时间早于在神经元的表达;另外OX-42阳性小胶质细胞和Fos阳性神经元在前脑分布基本相同,主要分布在大脑皮层、海马、杏仁核等部位。以上结果表明,前脑的小胶质细胞和神经元一样在戊四氮所致癫痫发作的早期表现明显的反应,但小胶质细胞反应的意义有待进一步研究。 相似文献
10.
大鼠延髓内神经激肽B受体神经元对腹膜伤害性刺激的Fos表达——免疫组织化学双重染色研究 总被引:1,自引:0,他引:1
为探讨神经激肽B受体(neurokininbreceptor,NKR)神经元在大鼠延髓的定位及其在腹膜伤害性传入信息整合中的作用。应用免疫组织化学双重染色方法,显示NKR神经元的分布并观察它们对腹膜化学伤害性刺激的Fos表达。结果表明:NKR样免疫反应(NKR-LI)神经元主要分布在孤束核(Sol)、延髓腹外侧区(VLM)、三叉神经脊束核尾侧亚核(Sp5C)浅层和三叉旁核(Pa5)。腹膜伤害性刺激激诱导的Fos表达神经元多数分布在Sol、VLM、Pa5和最后区。Sol、VLM和Pa5中约30.1%的NKR-LI神经元同时呈Fos样免疫反应。本研究提示:延髓内上述核团NKR-LI神经元与腹膜伤害性初级传入信息的整合有关,可能参与内脏伤害性刺激的抗伤害性感受(anti-noceciption)过程。 相似文献