沙门菌实时荧光定量PCR检测试剂盒的研制与应用

根据沙门菌保守的fimY基因序列设计合成了引物和TaqMan探针,建立了快速检测沙门茵的实时荧光定量方法,并对反应条件进行了优化,组装成快速检测试剂盒。通过对临床样品的检测,该试剂盒的检测灵敏度达4．5 CFU(25μL反应体系),比常规PCR高100倍,而且稳定性良好,至少可以冷冻保存9个月。结果表明,所建立的沙门菌实时荧光定量PCR检测试剂盒具有操作简便、快速、特异性强、灵敏度高、重复性好、稳定性强等优点,适于大量样品的检测。     

Public Service Motivation,Organizational Social Capital,and Knowledge Sharing in the Korean Public Sector

This article investigates whether public service motivation (PSM) and organizational social capital predict knowledge sharing in the public sector. The hypothesized relationships in the proposed model are verified with the online survey data of 506 public employees in Korea. The test results show that the two dimensions of PSM (attraction to public service and commitment to public values) and the trust component of organizational social capital are both positively related to knowledge sharing in the Korean public sector, and that the associability component of organizational social capital is indirectly associated with knowledge sharing through its influence on PSM. The article discusses the ways that PSM and organizational social capital may contribute to overcome the social dilemma of knowledge sharing in public organizations. It also suggests that there is need for further research on the individual dimensions of the PSM construct.     

Errors have been made,others will be blamed: Issue engagement and blame shifting in prime minister speeches during the economic crisis in Europe

This article investigates prime ministers’ communication strategies during the most recent economic crisis in Europe. It argues that when electoral risk is high but governments’ policy options are severely limited, prime ministers will use specific communication strategies to mitigate electoral risks. Two such communication strategies are analysed – issue engagement and blame shifting – by applying state-of-the-art quantitative text analysis methods on 5,553 speeches of prime ministers in nine European Union member states. Evidence is found for both strategies. Prime ministers talk about the economy more in response to both high (domestic) unemployment and low (domestic) gross domestic product growth. Furthermore, it is found that the (domestic) unemployment rate is the most consistent predictor of blame shifting: as the domestic unemployment rate goes up, this is followed by an increase in blame shifting towards banks, Greece and the Troika of the European Commission, the European Central Bank and the International Monetary Fund.     

Coming out of the shadow? Studies of local governments in Central and Eastern Europe in European academic research

This article is an analysis of the changing role of research on local governments in Central and Eastern Europe (CEE) within mainstream European studies. It refers to dependency theory (Wallerstein, Prebish) as applied to scientific research. It classifies CEE as being in the semi-periphery of academic research. The empirical analysis consists of two parts: (1) qualitative review of the most important of the comparative studies of European local governments. It includes a discussion on the role of local governments and researchers from CEE in these studies; (2) quantitative based on an analysis of articles published in international journals and citations for those articles in Scopus. The analysis covers 14 countries (all new member states who joined the EU from 2004 onwards, plus the Balkan countries: Albania, Macedonia, and Serbia).     

论矛盾转化中量变积累的形式与条件

矛盾转化是唯物辩证法的重要范畴。转化是质变,但有其量变积累,没有“渐进过程”,就不会有“中断”。探讨转化的量变积累的各种表现形式和条件,对于深化理解矛盾转化范畴的内涵具有重要意义。     

重庆市山羊博尔纳病病毒P24基因的检测

为了解重庆市山羊博尔纳病隐性带毒情况,分析博尔纳病病毒(BDV)的种系来源。采用巢式逆转录酶PCR结合荧光定量PCR(FQ-nRT-PCR)技术对重庆市60只山羊外周血单核细胞 (PBMCs)及脑组织中的BDV P24基因片段进行了检测,将阳性产物测序,并与国外BDV毒株进行了比较。结果,山羊外周血检测阳性率为8．3％(5／60),脑组织检测阳性率为10％(6／60)。该 BDV P24片段核苷酸序列与马源BDV H1766株同源性最高,达96．51％,与标准株Strain V和 He／80同源性为95．35％,并且编码的氨基酸序列也相同。表明,重庆市山羊中存在动物源性博尔纳病隐性带毒。该BDV P24核苷酸序列与Strain V和He／80株具有高度同源性。     

论我国中小企业界定标准的完善

为了扶植中小企业发展,各国均制定有促进中小企业发展的法律政策,这些法律政策首先要解决的问题就是对扶持对象——中小企业进行科学的界定。我国目前中小企业划分标准在实施中遇到了一些问题,应该采取定性和定量标准相结合的方式,重新界定小企业的内涵,简化中小企业的划分指标,细化中小企业划分标准,并将中小企业界定标准覆盖到全部第三产业。     

GC/MS和GC法定性定量分析可卡因

目的建立用于可卡因案件检验鉴定的GC和GC/MS定性、定量分析方法。方法通过选择和优化,建立GC、GC/MS法检验可卡因的最佳分析条件;用分别含0.6mg/ml地西泮为内标的0.10、0.20、0.40、0.60、0.80、1.00、1.20mg/ml可卡因标准品乙醇液,考察线性范围和方法检测限。结果分析方法线性方程:GC/FID,Y=1.055X-0.0021,R2=0.9999,GC/NPD,Y=0.556X-0.0016,R2=0.9996;可卡因检测限:GC/FID法10ng,GC/NPD法2ng;分别以所建GC/FID、GC/NPD分析方法和内标法对案件中缴获的可卡因毒品进行定量分析,结果为72%±2.3%,且两方法定量重现性良好。结论本文所建方法可以用于可卡因涉毒案件的检验鉴定。     

陕西省循环经济发展量化研究

随着资源和环境对经济发展的约束作用日益显现，全球范围内已逐步对可持续发展有了深入的体认。故而，基于此一理念的循环经济正逐步取代传统的已高消耗、高污染为表征的经济发展模式。本文基于经济和环境、能源、人口四者之间的量化分析，基本判断出陕西省的经济发展模式正处于在可持续发展理念指导下的向循环经济模式转型期，从而对我省的循环经济、生态文明建设提出对策。     

涉毒案件毒品定量鉴定是量刑的重要节点

涉毒案件中毒品的数量和含量是决定犯罪行为社会危害性大小的一个十分重要的因素,也是司法机关对犯罪分子课以刑罚的重要依据。毒品数量和含量认定的正确与否,关系到犯罪嫌疑人的犯罪的主观故意与案件量刑。当前,司法机关在办理的许多毒品犯罪（特别是可能判处15年以上刑罚）案件缺乏毒品定量鉴定结论,影响案件审理。笔者从法律和公平公正等方面探讨毒品含量分析的重要性。