Cell wall disruption: An effective strategy to improve the nutritive quality of microalgae in African catfish (Clarias gariepinus)

The rigid cell walls of microalgae may hinder their utilization in fish feeds. The current experiment assessed the correlation between the accessibility of microalgae nutrients and their in vivo digestibility in African catfish. Nannochloropsis gaditana biomass was subjected to physical or mechanical treatments to weaken its cell wall; untreated—no disruption treatment (UNT), pasteurization (PAS), freezing (FRO), freeze‐drying (FRD), cold pasteurization (L40) and bead milling (BEM). Six experimental diets formulated from differently treated and untreated microalgae (at 30% diet inclusion level) were tested on growth performance and apparent nutrient digestibility (ADCs) in juvenile African catfish. A basal diet (REF) containing no microalgae was used as reference diet. Results showed that biomass gain and feed conversion ratio of fish fed L40 and BEM diets increased by 13% and 11%, respectively, relative to the UNT diet. Additionally, FRD, FRO, L40 and BEM cell wall disruption treatments improved protein digestibility by 0.5%, 5.9%, 8.4% and 16.3%, respectively, compared to the UNT treatment. There was a positive correlation between accessibility of microalgal nutrients and their digestibility in African catfish. Nutrient digestibility of microalgae was dependent on extent of cell disruption. Also, the impact of cell disruption on nutrient digestibility of microalgae differs between African catfish and Nile tilapia.     

半根供铁对苹果砧木幼苗生长的影响

利用分根技术在水培条件下研究了半根供铁对苹果砧木幼苗生长的影响。结果表明,一半根系供铁不仅未影响植株对铁营养的需求,甚至在处理初期植株的铁吸收总量和铁吸收速率还略有提高。一半根系供铁苹果砧木小金海堂和山定子叶片活性铁质量分数均未降低,而且与对照相比无叶片黄化的现象。因此,通过半根供铁的方式来改善苹果砧木幼苗的生长,为进一步研究砧木缺铁适应性反应的调控部位打下基础,也为今后缺铁的矫正和提高铁素利用效率提供了一条新的思路。     

Earthworm biomass response to soil management in semi-arid tropical Alfisol agroecosystems

Earthworms recorded during 1989–1993 across 15 soil management treatments, comprising three different tillagexthree organic amendments (bare, farmyard manure, and rice straw) and six perennial ley treatments, belonged to two endogeic species, Octochaetona phillotti (Michaelsen) and Lampito mauritii Kinberg, while in a nearby undisturbed natural revegetation area three species were found, including the above two and Octonochaeta rosea (Stephenson). The earthworm biomass showed significant temporal and spatial variations and was higher during the post monsoon period than in the early rainy season. No worm biomass was recorded during the dry season. In the tillage and organic amendment treatments, the biomass was drastically reduced from September 1989 to September 1991 after the application of carbofuran and some herbicides, and was significantly reduced during these two years compared to that of 1992. The maximum monthly earthworm biomass ranged between 2.5 and 17.9 g m^-2 across the treatments and increased several-fold in the nearby natural revegetation area (75.9 g m^-2). It significantly increased in perennial ley treatments compared to annual treatments with organic amendments. Thus the earthworm biomass varied significantly (P<0.01) across the 15 treatments, indicating discernible effects of soil management.Visiting Scientist (under the Rockefeller Foundation Environmental Research Fellowship in International Agriculture)     

Effect of Different Treatment Rapeseeds on Activity of Cellulose Enzyme in Sheep

The purpose of the experiment is to research the effect of different rapeseed treatments feeding on activity of cellulose enzyme in sheep. Eight male adults of semi-fine wool sheep breeds with the same weight and permanent rumen cannulas were selected according to 4×4 Latin square design to determine the effect of different rapeseed treatment groups including crushed rapeseed, whole rapeseed, extruded rapeseed (dietary lipid content of =67 g · kg-1 ) and control group (dietary lipid content of =30 g · kg-1 ) on activity dynamic variation of ruminal celluolytic enzyme in sheep. The results showed that ruminal fluid pH matched up to the condition of fiber degradation and growth of cellulolytic bacterium when fed four diets; but there was significant difference (P0.05) for the activity of microcrystalline cellulose, glucanase, salicin enzyme involved in ruminal fiber degradation after fed 2, 4, 6 h.     

热处理对水牛乳稳定特性的影响

为比较不同热处理条件对水牛乳稳定性的影响,实验采用不同条件热处理后的pH缓冲容量、总溶解固体（total dissolved solids,TDS）、蛋白沉淀量及表观黏度等指标研究杂交高代水牛乳样品。结果表明：与未经处理的水牛乳样品相比,水牛乳经不同条件热处理后,其pH缓冲容量、TDS、蛋白沉淀量及表观黏度皆发生显著变化。其中水牛乳分别在经121℃/15 min和95℃/5 min处理后,其pH缓冲容量、TDS、蛋白沉淀量及表观黏度分别达到最大值和最小值。因此,认为在本实验研究范围内,水牛乳经121℃/15 min及95℃/5 min处理后,对其稳定性影响最大。     

遮阴处理对小蓬竹生长及生理特性的影响

为了探讨濒危植物小蓬竹对光照的适应性,设置轻度遮阴(LS,74.32％自然光)、中度遮阴(MS,44.83％自然光)、重度遮阴(SS,18.57％自然光)3种遮阴处理,以自然光为对照,观测了小蓬竹叶片生长及生理特性的变化.结果表明,相对于对照,轻度遮阴小蓬竹植株叶片数量显著增加,比叶重、可溶性蛋白、可溶性糖、可溶性淀粉、游离脯氨酸含量均无明显差异,总叶绿素、类胡萝卜素含量以及叶绿素b相对含量略有升高;中度遮阴及重度遮阴小蓬竹叶片相对数量、总叶绿素、类胡萝卜素、叶绿素b相对含量以及可溶性蛋白含量显著升高,比叶重、可溶性淀粉、游离脯氨酸含量显著降低,可溶性糖含量差异较小.说明小蓬竹对轻度遮阴环境有良好的适应性,适度遮阴更有利于其生长发育.遮阴加重的情况下,小蓬竹降低比叶重、促进扩展生长、提高总叶绿素尤其是叶绿素b的含量,从而提高光照截获面积与光吸收能力;调节游离脯氨酸含量维持正常的细胞膨压;减少光合有机物的净积累,将能量更多地用于生命活动的维持;保持足够的可溶性糖并提高可溶性蛋白的含量,从而加强对弱光逆境的抵御能力.由此推断,小蓬竹对中度及重度遮阴环境具有较强的适应性.     

不同药剂处理对猕猴桃根结线虫的防治效果

为了筛选出能够有效防治猕猴桃根结线虫的药剂。采用田间药剂施用筛选的方法,研究了12.5%阿维·噻唑膦EC等7种药剂对猕猴桃根结线虫的防治效果。结果表明供试的不同药剂对猕猴桃根结线虫均有一定的防效,能控制土壤中2龄幼虫的数量和抑制根结的形成,其中12.5%阿维·噻唑膦EC防治效果最好,药后45 d、180 d对根结线虫和二龄若虫的防治效果分别为78.40%、69.90%和83.83%、80.26%;10%噻唑膦GR与5%阿维菌素GR防效较好;2亿/毫升淡紫拟青霉+生物菌培养基B++(微生物肥)防效较差。12.5%阿维·噻唑膦EC、10%噻唑膦GR和5%阿维菌素GR可作为猕猴桃根结线虫防治的首选药剂。     

施肥对山地红枣林土壤微生物区系及酶活性的影响

采用田间试验与室内分析方法,研究施肥对山地红枣林土壤微生物区系及酶活性的影响。结果表明,施肥显著提高不同土层(0～20cm、20～40cm和40～60cm)中土壤微生物数量和酶活性(P<0.05),单一施肥与氮磷钾配施对土壤微生物区系和酶活性的影响有显著差异(P<0.05)。红枣林在施肥处理下土壤细菌、放线菌和真菌数量较CK样地均有不同程度增加:0～20cm土层微生物数量变化明显,其中N221.0P2O5272.7K2O 303.0和N303.7K2O 180.5两组处理的效果最明显。施肥处理下的土壤磷酸酶、蔗糖酶、脲酶和过氧化氢酶活性均比CK有显著增加(P<0.05),N221.0P2O5272.7K2O 303.0和N303.7K2O 180.5处理的土壤磷酸酶和蔗糖酶活性最高,P2O5272.7处理的土壤脲酶和过氧化氢酶活性最高。土壤纵向分析表明,0～60cm土层土壤细菌、放线菌和真菌数量和土壤脲酶、磷酸酶、蔗糖酶活性均随深度增加而降低。N221.0P2O5272.7K2O303.0配施处理能明显提高枣园土壤微生物数量及磷酸酶和蔗糖酶活性。     

不同施肥处理对黑土腐殖质组成的影响

以中国科学院海伦生态试验站为平台,利用小区长期定位试验研究了不同施肥处理在相同气候条件、轮作制度及相同管理措施下对黑土有机质积累及土壤腐殖质组分动态变化的影响.结果表明:有机肥与化肥配合施用不仅显著提高土壤中有机碳及胡敏酸的含量,而且能提高胡敏酸/富里酸(H_A/F_A)比值,促进土壤有机碳活化与更新,改善腐殖质的品质.施用化肥虽然也能在一定程度上提高土壤有机碳、胡敏酸等的含量,但其培肥效果明显低于有机肥与化肥配合施用.     

草本缓冲带优化配置对氮磷的去除效果

利用自行设计的小型试验装置模拟缓冲带,选择东北地区草木犀、白三叶、马唐、夏枯草4种常见植物,通过人工配水模拟农田径流水,采用草种不同栽种方式及草皮与其他生物处理方式相结合的方法,开展缓冲带对污染物的净化效果试验研究。结果表明,在渗流中,草种不同栽种方式和草皮与其他生物处理方式结合的缓冲带,3种混栽方式的NH4+—N、全P的去除效果最高分别为59.80%、56.81%。在径流中,草种不同栽种方式中3种混栽方式的NH4+—N、全P的去除效果最高分别为23.30%、22.30%;草皮与其他生物处理方式结合缓冲带,3种混栽+小沟对全P去除效果最高达27.4%,3种混栽+人工浮床对NH4+—N去除效果最高,达30.1%。在本试验条件下,3种混栽方式对渗流的净化效果较好,而草皮与其他生物处理方式结合的缓冲带对径流有较好的净化效果。