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Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound‐Propelled Nanomotors 下载免费PDF全文
Malthe Hansen‐Bruhn Dr. Berta Esteban‐Fernández de Ávila Dr. Mara Beltrán‐Gastélum Prof. Jing Zhao Dr. Doris E. Ramírez‐Herrera Pavimol Angsantikul Prof. Kurt Vesterager Gothelf Prof. Liangfang Zhang Prof. Joseph Wang 《Angewandte Chemie (International ed. in English)》2018,57(10):2657-2661
Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The Cas9/sgRNA complex is loaded onto the nanomotor surface through a reversible disulfide linkage. A 5 min ultrasound treatment enables the Cas9/sgRNA‐loaded nanomotors to directly penetrate through the plasma membrane of GFP‐expressing B16F10 cells. The Cas9/sgRNA is released inside the cells to achieve highly effective GFP gene knockout. The acoustic Cas9/sgRNA‐loaded nanomotors display more than 80 % GFP knockout within 2 h of cell incubation compared to 30 % knockout using static nanowires. More impressively, the nanomotors enable highly efficient knockout with just 0.6 nm of the Cas9/sgRNA complex. This nanomotor‐based intracellular delivery method thus offers an attractive route to overcome physiological barriers for intracellular delivery of functional proteins and RNAs, thus indicating considerable promise for highly efficient therapeutic applications. 相似文献
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Wei Zhe Teo Prof. Martin Pumera 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(42):14796-14804
As we progress towards employing self‐propelled micro‐/nanomotors in envisioned applications such as cargo delivery, environmental remediation, and therapeutic treatments, precise control of the micro‐/nanomotors direction and their speed is essential. In this Review, major emerging approaches utilized for the motion control of micro‐/nanomotors have been discussed, together with the lastest publications describing these approaches. Future studies could incorporate investigations on micro‐/nanomotors motion control in a real‐world environment in which matrix complexity might disrupt successful manipulation of these small‐scale devices. 相似文献
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Mimi Wan Qi Wang Xiaoyun Li Bo Xu Dan Fang Ting Li Yueqi Yu Leyi Fang Yue Wang Meng Wang Fenghe Wang Chun Mao Jian Shen Jia Wei 《Angewandte Chemie (International ed. in English)》2020,59(34):14458-14465
Limited tumor permeability of therapeutic agents is a great challenge faced by current cancer therapy methods. Herein, a kind of near infrared light (NIR)‐driven nanomotor with autonomous movement, targeted ability, hierarchical porous structure, multi‐drugs for cancer chemo/photothermal therapy is designed, prepared and characterized. Further, we establish a method to study the interaction between nanomotors and cells, along with their tumor permeability mechanism, including 2D cellular models, 3D multicellular tumor spheroids and in vivo models. In vivo tumor elimination results verify that the movement behaviour of the nanomotors can greatly facilitate them to eliminate tumor through multiple therapeutic methods. This work tries to establish systematic research and evaluation models, providing strategies to understand the relationship between motion behaviour and tumor permeation efficiency of nanomotors in depth. 相似文献
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Janus 粒子,也称为阴阳结构粒子或两面性非对称粒子,是指表面上具有两种或两种以上不同化学组成或性质的不对称粒子。目前,Janus 粒子因其独特的结构和功能已经逐渐成为生物医药、催化、材料以及防污等领域中的新型功能材料。在环境检测领域,Janus材料亦因其特殊的光学、磁学及电学性能,为提高检测灵敏度、选择性和稳定性等提供了新的研究方向。基于此,本文主要讨论了Janus材料在环境检测方面的特点、优势和相关应用。最后,本文基于本课题组的研究经验以及工作中所面临的问题,对本领域的发展和未来的研究方向提出了展望,以期对本领域的未来发展提供指导。 相似文献
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Dr. Samuel Sánchez Dr. Lluís Soler Jaideep Katuri 《Angewandte Chemie (International ed. in English)》2015,54(5):1414-1444
Chemically powered micro‐ and nanomotors are small devices that are self‐propelled by catalytic reactions in fluids. Taking inspiration from biomotors, scientists are aiming to find the best architecture for self‐propulsion, understand the mechanisms of motion, and develop accurate control over the motion. Remotely guided nanomotors can transport cargo to desired targets, drill into biomaterials, sense their environment, mix or pump fluids, and clean polluted water. This Review summarizes the major advances in the growing field of catalytic nanomotors, which started ten years ago. 相似文献
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Inside Cover: Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound‐Propelled Nanomotors (Angew. Chem. Int. Ed. 10/2018) 下载免费PDF全文
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Tieyan Si Xian Zou Zhiguang Wu Tianlong Li Xin Wang Krasnyuk Ivan Ivanovich Qiang He 《化学:亚洲杂志》2019,14(14):2460-2464
We report the bubble dragged microrocket consisting of functionalized multilayer polymer covered asymmetrically by platinum nanoparticles. The microrocket is pushed back during bubble growth over a small step and dragged forward over a big step during bubble explosion. Each bubble explosion induced a shock wave of gas which propagates in water at ultrafast speed. The bubble dragged microrocket can move along an approximate straight line instead of a fluctuating circle which is the trajectory of a bubble‐pushed microrocket in most cases, which makes it a promising candidate for drug delivery and simulating rod‐shaped bacteria. 相似文献
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