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用两种相似多糖类手性固定相拆分阿托品外消旋对映体的比较 总被引:1,自引:0,他引:1
分别涂敷纤维素-三(3,5-二甲基苯基氨基甲酸酯)(CDMPC)与直链淀粉-三(3,5-二甲基苯基氰基甲酸酯)(ADMPC)于自制的球形氨丙基硅胶上,制备了两种多糖类手性崮定相。用高效液相色谱法(HPLC)在正相条件下,用两种固定相直接拆分了阿托品(atmpine)外消旋体、在正己烷中加入了不同的醇类改性剂对阿托品进行拆分,并优化了流动相中醇类改性剂的比例:结果发现,阿托品在CDMPC固定相上可以得到基线拆分,而在ADMPC固定相上只能得到部分拆分。 相似文献
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本文对微型管状离子选择电极在流动条件下的电化学特征进行了研究,并设计了新的集成微管路离子选择以电极功能块。用此微型装置测定了土壤、血清、水和药物中的K~+、Na~+、pH、Cl~-、F~-、阿托品、东茛菪碱,并和各种标准方法作了比较,获得满意分析结果。 相似文献
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阿托品选择电极的研制和应用 总被引:2,自引:0,他引:2
报道了一种阿托品选择性电极的研制和应用,该电极是将对阿托品敏感的聚氯乙烯膜涂敷于pH玻璃电极球部表面而制成。在0.10mol/L,磷酸盐冲浓度(pH4.0)中,所制电极对阿托品的Nernst响应线性范围为1.70×10^-5~2.00×10^-2mol/L阿托品,响应斜率为55.8mV,电极响应时间小于45s,使用寿命大于6个月,而且具有制作简单,使用方便的特点,已将该电极应用于硫酸阿托品制剂中测 相似文献
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The coupling of Ru(bpy)32+ based electrochemiluminescence (ECL) detection with capillary electrophoresis (CE) was developed for the simultaneous determination of the two major active ingredients (atropine and scopolamine) in Flos daturae. Parameters related to the separation and detection were discussed and optimized. It was proved that 20 mM phosphate buffer at pH 8.48 could achieve the most favorable resolution, and the high sensitivity of detection was obtained by maintaining the detection potential at 1.2 V. Under the optimized conditions: ECL detection at 1.2 V, 20 mM phosphate buffer at pH 8.48, 5 mM Ru(bpy)32+ and 50 mM phosphate buffer at pH 7.48 in the detection reservoir, detection limits of 5 × 10−8 mol/l for atropine and 1 × 10−6 mol/l for scopolamine were obtained. Relative standard derivations of the ECL intensity and the migration time were 5.16 and 0.71% for atropine and 5.07 and 1.22% for scopolamine, respectively. Developed method was successfully applied to determine the amounts of both alkaloids in Flos daturae. A baseline separation for atropine and scopolamine was achieved within 11 min. 相似文献
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郁金对胃平滑肌条运动的影响 总被引:1,自引:0,他引:1
目的:观察利胆药物——郁金对兔离体胃平滑肌的影响.方法:取兔胃肌条,安置在各恒温灌流肌槽中并用BL-310生物技能实验系统记录胃各部平滑肌条的收缩活动.结果:郁金显著升高兔胃底和胃体纵行肌条张力,减小胃体收缩波平均振幅,并有剂量依赖关系.结论:郁金对胃肌条收缩活动具有明显的兴奋作用,这种兴奋作用部分经由胆碱能M受体介导. 相似文献
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This work developed a simple and sensitive method for simultaneous determination of three effective ingredients, atropine, scopolamine and anisodamine, in Flos daturae based on capillary electrophoresis coupled with electrochemiluminescence detection. β‐Cyclodextrin was used as an additive to the running buffer for obtaining the absolute separation. The proposed method displayed the linear ranges from 0.2 to 100, 0.2 to 100 and 20 to 200 μM for anisodamine, atropine and scopolamine with correlation coefficients more than 0.99, respectively. This method showed the relative standard deviations less than 4% and 6% for detection of migration time and peak height, respectively, and was suitable for the determination of these tropane alkaloids in plants and valuable in clinical and biochemical laboratories for quality control. 相似文献
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A new post chemiluminescence (PCL) reaction was observed when atropine sulfate was injected into the reaction mixture after the finish of CL reaction of Ce(IV) and sodium sulfite. The possible mechanism for the PCL reaction was discussed via the investigation of the CL kinetic characteristics, the CL spectra, the UV absorption spectra and the fluorescence spectra of some related substances. The flow injection PCL method for the determination of atropine sulfate was established. The relative standard deviation (R.S.D.) was 2.8% (n = 11, c = 5.0 × 10−6 g mL−1). The PCL intensity responded linearly to the concentration of atropine sulfate in the range 1.0 × 10−6 to 5.0 × 10−5 g mL−1 with a linear correlation of 0.9947. The detection limit was 4 × 10−7 g mL−1 atropine sulfate. The method had been applied to the determination of atropine sulfate in the tablets and the results were consistent with the method of Chinese pharmacopoeia. 相似文献
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Baiqing Yuan Chunying Zheng Hong Teng Tianyan You 《Journal of chromatography. A》2010,1217(1):171-174
A rapid and simple method was demonstrated for the analysis of atropine, anisodamine, and scopolamine by nonaqueous capillary electrophoresis (NACE) coupled with electrochemiluminescence (ECL) and electrochemistry (EC) dual detection. The mixture of acetonitrile (ACN) and 2-propanol containing 1 M acetic acid (HAc), 20 mM sodium acetate (NaAc), and 2.5 mM tetrabutylammonium perchlorate (TBAP) was used as the electrophoretic buffer. Although a short capillary of 18 cm was used, the decoupler was not needed and the separation efficiency was good. The linear ranges of atropine, anisodamine, and scopolamine were 0.5–50, 5–2000, and 50–2000 μM, respectively. For six replicate measurements of 100 μM scopolamine, 15 μM atropine, and 200 μM anisodamine, the RSDs of ECL intensity, EC current, and migration time were less than 3.6%, 4.5%, and 0.3%, respectively. In addition, because the organic buffer was used, the working electrode (Pt) was not easily fouled and did not need reactivation. The method was also applied for the determination of these three alkaloids in Flos daturae extract. 相似文献