Characterization of microbial communities from coastal waters using microarrays

Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish.     

Molecular behavior and interactions with microbes during anaerobic degradation of bio-derived DOM in waste leachate

It is the key to control bio-derived dissolved organic matters (DOM) in order to reduce the effluent concentration of wastewater treatment, especially for waste leachate with high organic contaminants. In the present study, the anaerobic degradation of aerobically stabilized DOM was investigated with DOM substrate isolated through electrodialysis. The degradation of bio-derived DOM was confirmed by reduction of 15% of total organic carbon in 100 days. We characterized the molecular behavior of bio-derived DOM by coupling molecular and biological information analysis. Venn based Sankey diagram of mass features showed the transformation of bio-derived DOM mass features. Occurrence frequency analysis divided mass features into six categories so as to distinguish the fates of intermediate metabolites and persistent compounds. Reactivity continuum model and machine learning technologies realized the semi-quantitative determination on the kinetics of DOM mass features in the form of pseudo-first order, and confirmed the reduction of inert mass features. Furthermore, network analysis statistically establish relationship between DOM mass features and microbes to identify the active microbes that are able to utilize bio-derived DOM. This work confirmed the biological technology is still effective in controlling recalcitrant bio-derived DOM during wastewater treatment.     

加强云南环境保护宣传教育力度--积极开创"绿色社区"

从云南环境宣传教育现状出发,分析了目前在云南创建“绿色社区”的现实意义、必要性,并提出了创建的评选标准细则和评选的基本办法,为今后云南创建“绿色社区”提供参考性意见。     

多层商住楼抗震设防投资效益评价

针对不同结构多层商住楼的具体特征，运用结构易损性分析方法对各类房屋未来的震害情况进行了分析，给出了震害损失与投资效果的综合评价方法，为进行这类房屋的结构选型、设计及工程投资的优化提供一定的理论依据。     

论嵌套制——一种安全生产管理的新方法

笔者所论述的嵌套制 ,是继现有 5种工厂管理运行机制之后的第 6种机制。通过阐述嵌套制的特征、原理、实例、理论与现有的 5种机制的区别 ,证明它是一种有效的工厂安全生产管理机制。推行嵌套制 ,对于追求更切合危险源点的潜在性与复杂性的控制方案 ,对于解决职业健康安全管理体系的路径与工厂其他各种管理途径接轨的问题 ,对于提升职业健康安全管理体系的实用性 ,对于催生具有实质效果的各级、各层次安全监督管理机制 ,对于促进安全生产管理理论的发展 ,都具有重要的作用。     

一种DSP+FPGA实时信号处理系统

简要叙述了常用的信号处理系统的类型与处理机结构,介绍了正逐步得到广泛应用的DSP+FPGA处理机结构,在此基础上提出了一种实时信号处理的线性流水阵列,并举例说明了该结构的具体实现,最后分析说明了此结构的优越性。     

县域经济所有制结构的调整

县域经济的所有制结构所表现出来的经济实体形式包括县属地方国有经济、多种形式的集体经济和民营经济,探讨了县域经济所有制结构的形式及其在市场经济中的作用.     

拉索预应力空间网格结构的索张力计算及张拉全过程分析

实际工程中,对于拉索预应力空间网格结构的索张拉是一个需要反复调整的繁琐过程。对此,提出了一种针对多阶段多批次张拉施工全过程分析的计算方法,用以提高索张拉施工的效率。该法通过不断减小拉索实际张力与目标张力的差值来求解各索在张拉过程中应该产生的初始应变,最后只要利用计算结果并按照预定的顺序张拉,便可使所有索只经过一次张拉就能最终达到各自的目标张力值。对一个150 m跨度的拉索预应力球面巨型网格结构进行了计算,并利用计算结果,对结构的杆件内力和最大节点位移进行了张拉全过程跟踪分析。结果表明,在实际工程中采用上述方法进行计算与张拉是切实可行的,另外,在张拉过程中进行跟踪分析十分必要。     

混合接种PN与PN/A颗粒污泥快速启动连续流自养生物脱氮反应器

在连续流条件下,快速培养亚硝化-厌氧氨氧化（PN/A）颗粒污泥是实现污水高效生物脱氮处理的关键技术. 与PN/A污泥相比,亚硝化（PN）颗粒污泥具有生长周期短、易于批量化培养的优点,并可作为富集厌氧氨氧化菌（AMX）的载体. 在3个完全混合流反应器（R1~R3）中,分别按照质量比3∶1、1∶1和1∶3混合接种PN/A和PN颗粒污泥,并通过设置高氨氮负荷、短水力停留时间和强水力剪切条件,成功启动了连续流自养生物脱氮工艺. 结果表明,尽管R3的启动时长较R1和R2更长,但污泥接种比并未显著影响连续流反应器在稳定状态下的脱氮性能,总氮去除负荷均可达到2.6 kg·（m³·d）^-1以上. 接种的PN颗粒污泥通过提供好氧氨氧化菌种（AOB）,为AMX生长供给了充足的亚硝态氮基质,充分发挥了培养PN/A颗粒污泥的前驱体作用. 由高通量测序结果可知,R1~R3中成熟颗粒的微生物丰度和多样性指数均明显高于接种污泥. AOB（Nitrosomonas属）和AMX（Candidatus Kuenenia和Brocadia属）与Chloroflexi、Bacteroidetes和Chlorobi等异养菌门是驱动自养生物脱氮和维持颗粒结构稳定的关键菌群. 总之,PN与PN/A颗粒污泥的混合接种是快速启动连续流自养脱氮工艺的可行策略,对工程应用具有指导意义.     

长春市不同土地利用生境土壤昆虫幼虫群落组成与生态分布

对长春市郊区天然次生林、市内公园风景林、郊区防护林和农田等土地利用生境进行土壤昆虫幼虫调查,研究土地利用差异对土壤昆虫幼虫群落特征的影响。研究区共捕获土壤昆虫幼虫35科411只,其中鞘翅目幼虫14科319只,双翅目幼虫13科64只,鳞翅目幼虫8科28只。研究结果表明:不同土地利用生境土壤昆虫幼虫群落组成与生态分布存在差异,防护林和农田土壤昆虫幼虫的类群数和个体密度显著减少;除农田外,其他生境土壤昆虫幼虫群落个体密度和类群数主要集中分布在0～5cm土壤层,生境凋落物的移除对土壤昆虫幼虫群落组成影响不明显;此外,鞘翅目、双翅目和鳞翅目幼虫对土地利用和植被类型变化的响应也不同。