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双酚-A对Medaka的类雌激素效应研究 总被引:7,自引:1,他引:7
利用Medaka进行了双酚-A与17β-雌二醇的短期暴露研究.研究结果发现实验室建立的流水式暴露体系可为实验提供连续、恒定的污染物暴露浓度.利用肝肉指数可灵敏响应和指示污染物暴露.双酚-A可以剂量相关的方式诱导雄性Medaka肝脏产生卵黄蛋白原,从而有效证实了其类雌激素效应. 相似文献
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Xi Haiyan Cai Qiang He Miao Shi Hanchang 《Frontiers of Environmental Science & Engineering in China》2007,1(3):381-384
This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations
of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 104 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.
Translated from Environmental Science, 2005, 26(5): 128–131 [译自: 环境科学] 相似文献
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A Characterization of Selected Endocrine Disruptor Compounds in a Portuguese Wastewater Treatment Plant 总被引:1,自引:0,他引:1
Maurício R Diniz M Petrovic M Amaral L Peres I Barceló D Santana F 《Environmental monitoring and assessment》2006,118(1-3):75-87
Anthropogenic compounds that are able to disrupt the endocrine system of wildlife species are a major cause for concern and
have led to a demand for new screening methods. The identification and quantification of endocrine disruptor compounds at
wastewater treatment plant is of major interest to assess the endocrine activity of wastewater treatment plant discharges
into the environment. This study consists of a preliminary survey of concentrations of previously selected endocrine disruptor
compounds, undertaken to establish environmental concentrations and to support a biological program assay exposing freshwater
fish to them. Selected endocrine disrupting chemicals (APEs, bisphenol A and 17 β-estradiol) were measured in samples from a wastewater treatment plant located in Lisbon (Portugal), using recent commercial
enzyme-linked immunosorbent assay kits and also LC-MS/MS. The results show that the wastewater treatment plant treatment process
is efficient on the removal of target endocrine disruptor compounds. However, environmentally significant concentrations are
still present in the treated effluent. The results also show that enzyme-linked immunosorbent assay kit is suitable for routine
analysis of the selected compounds. The results are also useful since the wastewater treatment plant is located in a Mediterranean
region, which results in an effluent with its own characteristics. 相似文献
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Jane C. Chuang Jeanette M. Van Emon Margaret E. Tefft Nancy K. Wilson 《Journal of environmental science and health. Part. B》2013,48(6):516-523
A low-cost, high throughput bioanalytical screening method was developed for monitoring cis/trans-permethrin in dust and soil samples. The method consisted of a simple sample preparation procedure [sonication with dichloromethane followed by a solvent exchange into methanol:water (1:1)] with bioanalytical detection using a magnetic particle enzyme-linked immunosorbent assay (ELISA). Quantitative recoveries (83–126 %) of cis/trans-permethrin were obtained for spiked soil and dust samples. The percent difference of duplicate ELISA analyses was within ± 20 % for standards and ± 35 % for samples. Similar sample preparation procedures were used for the conventional gas chromatography/mass spectrometry (GC/MS) analysis except that additional cleanup steps were required. Recoveries of cis/trans-permethrin ranged from 81 to 108 % for spiked soil and dust samples by GC/MS. The ELISA-derived permethrin concentrations were highly correlated with the GC/MS-derived sum of cis/trans-permethrin concentrations with a correlation coefficient (r) of 0.986. The ELISA method provided a rapid qualitative screen for cis/trans-permethrin in soil and dust while providing a higher sample throughput with a lower cost as compared to the GC/MS method. The ELISA can be applied as a complementary, low-cost screening tool to prioritize and rank samples prior to instrumental analysis for exposure studies. 相似文献
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为了进一步探索种群水平的生态风险评估方法,本文利用β-N-Acetyl-D-glucosaminidase(NAGase)的变化量来监测农药对摇蚊种群发育的影响。从花翅摇蚊Chironomus kiiensis体内分离纯化得到电泳纯的NAGase,并通过免疫大白兔制得NAGase的多克隆抗体。运用间接非竞争ELISA法检测抗体特异性,结果表明其与共同存在于水体的一些生物的NAGase的交叉反应率为隆线溞4.41%、老年低额溞3.12%、多刺裸腹溞3.40%、中华薄壳介4.17%、日本沼虾3.23%、白纹伊蚊7.50%、小球藻0.5%。运用抗体测得毒死蜱、氰戊菊酯和阿维菌素3种杀虫剂对于摇蚊NAGase释放量的12 d-EC50分别为1.2012、0.0043和0.6281μg·L-1,以NAGase活力作为测试终点,测得相应的12 d-EC50:1.4765、0.0051和0.6756μg·L-1,两者差异不显著,但均显著低于以死亡作为测试终点的12 d-LC50:4.8171、0.0954和2.1340μg·L-1,且毒力大小均为氰戊菊酯阿维菌素毒死蜱。上述结果表明,利用NAGase多克隆抗体可以特异性地检测农药对花翅摇蚊种群发育的影响。 相似文献
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Due to the wide occurrence of endocrine disrupting chemicals in the environment, it is much of importance to develop high throughput screening method for the analysis of this kind of pollutants. Using anion-exchange membrane chromatography, vitellogenin (VTG) from the plasma of 17β-estradiol (E2) treated Chinese rare minnow was rapidly purified within 15 min. Both polyclonal antibody (PcAb) and monoclonal antibody (McAb) against rare minnow VTG (R-VTG) were prepared in rabbit and Balb/c mice, respectively. The competitive enzyme-linked immunosorbent assays (ELISA) based on either PcAb or McAb were developed to identify and quantify R-VTG in the plasma, and these two methods showed similar characteristics. The detection limits of both assays were lower than 3 ng mL−1 with the working ranges covering three magnitudes. The recovery efficiencies of PcAb and McAb based ELISA were 104.2% and 102.6%, respectively; and the intra-assay and inter-assay of these two assays were 6.2% and 9.2%, 8.6% and 12.8%, respectively. These results indicated that the described competitive ELISA methods were sensitive and valuable tools for quantifying vitellogenin in rare minnow plasma. These methods were then applied to measure R-VTG concentrations in plasma of male fish exposed to a series of E2 concentrations. When E2 levels were less than 10 ng L−1, R-VTG levels in plasma were comparable to that in solvent control, while R-VTG levels significantly increased 15-folds and 350-folds, respectively when E2 exposure concentrations were controlled at 10 and 50 ng L−1. The high sensitivity of Chinese rare minnow to E2 was demonstrated, making it a valuable model species to study environmental estrogens. 相似文献
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A highly sensitive enzyme immunoassay was standardized for aflatoxin B1 determination in poultry feed and its components using Riedel-de-Haen, ELISA Systems. A microtitration plate method was optimized using anti-aflatoxin B1 antibodies and peroxidase – aflatoxin B1 conjugate, based on competitive enzyme immunoassay principle. Standards of concentrations of 5, 10, 20, 50, 100, 500?ng/L aflatoxin B1, prepared in phosphate-buffered saline, were used. Standard curves showed that as the concentration of antigen decreased, absorbance values increased. Fifty percent inhibition was observed at 37?ng/L. Regression analysis showed that log concentration was inversely related to %B/B0, with a highly significant negative correlation (?0.980). The lowest detection limit for aflatoxin B1 was 5?ng/L. Using this standardized ELISA, aflatoxin B1 was detected in most of the commercially available poultry feed samples and their components. The data suggest that this test is suitable for the accurate determination of aflatoxin B1 concentrations in poultry feed and its components. 相似文献