Impregnation of silk sericin into polyester fibers using supercritical carbon dioxide

Silk sericin was impregnated into polyester fabric using supercritical carbon dioxide (SCCO₂) to overcome polyester hydrophobicity. The effects of sericin molecular weight, pH of sericin, solution and cosolvent types on sericin impregnation were investigated. Enzyme‐hydrolyzed, acid‐, based‐hydrolyzed sericin in SCCO₂, and a 30 kDa sericin in SCCO₂ modified with cosolvents such as water, methanol, 1‐propanol, and acetone; and a modifier: sodium hydroxide solution were used in this work. Impregnation of sericin in polyester was indicated by Fourier transform infrared spectrophotometry (FTIR) and dyeing with acid dye. Degradation of polyester fibers during SCCO₂ process was indicated by scanning electron microscopy (SEM). Methylene blue dyeing was used to realize carboxyl group in polyester. The results showed no impregnation of sericin into polyester by using SCCO₂ modified with cosolvents. However, sericin was impregnated into modified surface polyester since hydrophilic groups such as carboxyl and hydroxyl groups were regenerated by alkaline hydrolysis. Samples impregnated with hydrolyzed sericin showed high color strength of Supranolechtbordeaux B acid dye. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007     

Modification of cellulose fiber with silk sericin

Cellulose fiber surface was modified with silk sericin (or simply, sericin). Sericin fixation on cellulose was confirmed by environmental scanning electron microscopy (ESEM) and Fourier transform infrared spectrophotometry–attenuated total reflectance (FTIR‐ATR). Sericin content in finished samples was estimated by dyeing treated fabrics with an acid dye, Supranol Bordeaux B, and determining K/S and L values of the dyed fabrics. The treated fabrics were tested for free formaldehyde content, crease recovery, tensile strength, electrical resistance, water retention, and biocidal activity. From ESEM and FTIR‐ATR results, it was found that sericin coated onto cotton surfaces as a film. Increasing sericin content in the finishing solution increased the amount of coated sericin, and a greater depth of color in dyed samples and reduced free formaldehyde content in treated samples were observed. The sericin content in samples was found to have a negligible influence on tensile strength and crease recovery angle. With increasing sericin content, electrical resistivity of the samples dramatically decreased and water retention increased, indicating that sericin‐treated fabrics may be comfortable to wear because of its maintenance of moisture balance with respect to human skin. Because cotton textile coated with sericin exhibited low formaldehyde content and no biocidal activity against Klebsiella pneumoniae and Staphylococcus aureus, the fabric may reduce skin irritation and disturbance of physiological skin flora arising from textile contact. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 1421–1428, 2005     

Silk Sericin Enrichment through Electrodeposition and Carbonous Materials for the Removal of Methylene Blue from Aqueous Solution

The recycling and reuse of biomass waste for the preparation of carbon-based adsorbents is a sustainable development strategy that has a positive environmental impact. It is well known that a large amount of silk sericin (SS) is dissolved in the wastewater from the silk industry. Utilizing the SS instead of discharging it into the environment without further treatment would reduce environmental and ecological problems. However, effective enrichment of the SS from the aqueous solution is a challenge. Here, with the help of carboxymethyl chitosan (CMCS), which can form a gel structure under low voltage, an SS/CMCS hydrogel with SS as the major component was prepared via electrodeposition at a 3 V direct-current (DC) voltage for five minutes. Following a carbonization process, an SS-based adsorbent with good performance for the removal of methylene blue (MB) from an aqueous solution was prepared. Our results reveal that the SS/CMCS hydrogel maintains a porous architecture before and after carbonization. Such structure provides abundant adsorption sites facilitating the adsorption of MB molecules, with a maximum adsorptive capacity of 231.79 mg/g. In addition, it suggests that the adsorption is an exothermic process, has a good fit with the Langmuir model, and follows the intra-particle diffusion model. The presented work provides an economical and feasible path for the treatment of wastewater from dyeing and printing.     

戊二醛固着茧衣丝胶的工艺研究

用戊二醛作固着剂对茧衣进行丝胶固着,通过对戊二醛浓度和固胶温度的探索,得出了在茧衣不明显着色的前提下戊二醛固胶的较优工艺方案。     

丝绸废水中丝胶提取方法的研究

丝绸废水经过真空浓缩和喷雾干燥制成粗粉末。采用酸析法和低温乙醇沉淀法对粗粉末中的丝胶进行提取,结果表明:低温乙醇沉淀法的提取率较高,采用体积分数为75%的乙醇溶液提取率达到64%。理化性质分析结果表明,低温乙醇沉淀法提取的丝胶蛋白含量高达90%以上,溶解度有所下降,灰分含量降低,蚕腥味被去除。应用圆二色光谱和红外光谱研究2种方法提取的丝胶二级结构分子构象的变化,结果显示提取得到的丝胶蛋白分子构象以无规卷曲为主,β转角构象的比例增加。研究结果表明,低温乙醇沉淀法是一种简便有效提取丝胶的方法。     

丝胶蛋白酶解产物抗氧化活性的研究

本实验采用六种蛋白酶对丝胶蛋白进行酶解,并分析其酶解产物清除二苯代苦味酰基 (DPPH) 自由基能力.通过脂质过氧化体系、还原力、金属螯合能力评价其酶解4h酶解产物的抗氧化活性.结果表明:各种酶解产物都呈现一定的清除DPPH自由基的能力,其中以碱性蛋白酶最强 (p＜0.05);各种酶解产物都存在抑制脂质过氧化活性,与其他蛋白酶相比碱性蛋白酶的抑制活性显著的高于其他酶 (p＜0.05);各种蛋白酶解产物具有一定的还原力和金属螯合能力.这些结果表明:六种酶解产物都具有显著的抗氧化活性.     

增塑剂对乳清蛋白-丝胶复合可食用膜性能的影响

本实验研究了甘油、山梨醇、聚乙二醇200以及甘油,聚乙二醇400(3/1)的混合物作为增塑剂对乳清蛋白-丝胶复合膜性能的影响.结果表明:增塑剂对乳清蛋白-丝胶复合膜的性能有显著影响.同一种增塑剂随着添加量的升高,膜的拉伸强度和透明度降低,断裂拉伸率、水蒸气透过性、含水量和溶解性升高.含有山梨醇和含有聚乙二醇200的膜的拉伸强度和溶解性比含有甘油和含有甘油/聚乙二醇400(3/1)的膜大,但前两者的拉伸率均较小.不同增塑剂制成的膜的水蒸气透过性和水分含量排列顺序为:含有甘油的膜＞含有甘油,聚乙二醇400(3/1)的膜＞含有乙二醇200的膜＞含有山梨醇的膜.甘油和甘油,聚乙二醇400(3/1)的膜相比,后者在一定添加量下拉伸性和阻水性都要优于前者.     

丝厂废水中的丝胶蛋白在超滤过程中的膜污染和膜清洗

采用十种不同的膜清膜方法对超滤丝厂废水后被污染的中性聚砚中空纤维超滤膜进行了研究。结果表明：０．１％ＮａＯＨ、０．５％ＮａＣｌＯ、０．１％渗透剂等组成的碱性溶液对丝胶蛋白造成的膜污染具有最佳清洗效果。     

棉织物的丝胶涂层整理及其性能

为开发高品质、功能性的棉制品,采用带负电荷的丝胶蛋白与带正电荷的壳聚糖衍生物在水溶液中发生反应,生成具有一定胶黏作用的新型微颗粒,再通过聚丙烯酸类低温交链剂进一步将丝胶固着在棉织物上。对整理前后棉织物的服用性能、表面形态等进行测试,结果表明:丝胶均匀涂覆在棉织物上,且耐洗性良好,洗涤5次后的溶失率约为0.21%,丝胶整理后棉织物的吸放湿性、保温性、透湿性和抗菌性均有提高,透气性基本不变,是一种新型高档棉制品。