Divide-and-Conquer Matrisome Protein (DC-MaP) Strategy: An MS-Friendly Approach to Proteomic Matrisome Characterization

Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins.     

航空发动机复杂扭曲叶片分步电解加工研究

为实现复杂扭曲叶片在较小间隙下的稳定加工,提出了一种分步式电解加工的工艺方法,建立了复杂扭曲叶片小间隙(0.2 mm)和大间隙下(0.5 mm)扭曲流道的流道模型,采用有限元法进行了流场仿真研究,结果表明增加流道间隙可以解决流道中涡流和流场紊乱的问题。开展了复杂扭曲叶片小间隙连续式加工和分步式加工的试验研究。结果表明,采用小间隙连续式加工,当阴极进给至3.8 mm位置时,在叶片排气边靠近叶根流道扭曲处出现短路打火情况;而采用分步式加工方式能够实现加工的顺利进行。     

DVB条件接收系统中独立加扰器的设计与实现

本文介绍DVB条件接收系统的基本结构，详细分析了条件接收系统的工作原理，并给出条件接收系统中独立加扰器SAS(Stand Alone Scrambler)的具体实现方案。     

LASER ENHANCED ELECTROCHEMICAL MACHINING PROCESS

An experimental apparatus was designed and built for the hybrid process of laser enhanced electrochemical machining (ECM). Comparison experiments had been done between solely ECM and laser enhanced ECM. The machining performance of hybrid process was investigated and compared with solely ECM. The principle of the hybrid process was exposed by analyzing the microstructure of machining surface. The results show that the hybrid process has advantages in machining accuracy, surface quality, and machining efficiency.     

基于Ansys的电化学加工辅助阴极设计

根据电化学加工极间间隙电势分布的拉普拉斯方程，提出了一种阴极设计算法－误差调整法。该算法的实现主要采用了Ansys有限元计算软件。计算结果表明：该法计算迭代次数较为适当，对于精度要求不高的电化学加工阴极设计，基于Ansys的辅助阴极设计有效可行。     

可重构电解加工机床研究

电解加工机床通常是根据用户具体需求单机生产，机床研制周期长、设计重复、成本高。为此，提出采用开放式控制系统、模块化思想开发可重构电解机床，并对机床的机械部分、控制系统及通信系统进行了研究。     

基于双极膜水解离的超纯水电解加工研究

研究了双极膜在超纯水中的工作特性,提出了应用双极膜水解离进行超纯水电解加工的方法,并进行了加工实验.结果表明:在强电场作用下,双极膜促使了水的解离,电流密度可达到2.8 A/cm2,实现了小孔的超纯水电解加工.     

电子对抗力量实施协同作战问题之探讨

从电子对抗力量体系的构建原则入手,结合电子对抗作战特点,对运用电子对抗力量与其他作战力量协同作战问题进行了探讨,就提高电子对抗力量协同作战能力提出了一些基本措施.     

基于网络中心战的空军电子对抗作战运用

传统以平台为中心的电子对抗作战方式是点对点的对抗,无法满足体系对抗的需要,以网络为中心的空军电子对抗作战运用在情报共享的基础上,合理、高效调用作战资源,使得作战方式更加灵活,作战效能更加突出.基于网络中心战的基本概念,探讨了基于网络中心战的机载电子对抗作战方式,由此得到了几点启示.     

2-D Doppler shift-frequency intermittent sampling jamming against SAR

To generate the false targets in the desired area accurately, a novel two dimensional(2-D) intermittent sampling jamming algorithm against synthetic aperture radar(SAR) is presented. The intercepted radar signals are processed via 2-D intermittent sampling and Doppler shift-frequency and retransmitted to radar. Hence, the false target-lattice emerges in the specified area. The generation mechanism, position and distribution characteristics of false target-lattice are discussed. Meanwhile, the reason for the asymmetric amplitude of the target false-lattice and the modulation factor of energy compensation are presented. The correctness of theoretical analysis and validity of the proposed algorithm are verified through simulation experiments. Compared with conventional methods, the critical requirement of the jammer position can be reduced by the proposed method.