EFFECT OF NITRATE AND PHOSPHATE ON ACCUMULATION OF β-CAROTENE ISOMERS IN DUNALIELLA SALINA

DunaIiella salina, a halotolerent unicellular green alga, can accurmulate a Iarge amount of β-caroteneunder environmental conditions. The isorners of β-carotene extIacted from D. salina culturedin medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-Talumins column. At least six isomers were found in different proportions depending on the culture me-dia's nitrate and/or phosphate concentrations. Nitrate and/or phosphae defidency was conducive tothe accumulation of totaI cis isomers but not of al1 trans isomer. lt is sUggeSted that 1 mmol/L KNO_3and 0.1 mmol/L KH_2, PO_4 are favourable for accumulation of total cis β-carotene.     

大黄鱼(Pseudosciaena crocea)转铁蛋白(Tf)基因克隆及序列分析

提要应用RNeasy animal mini试剂盒抽提大黄鱼total RNA,逆转录合成模板cDNA;同时综合分析从GenBank数据库查询到已报道的转铁蛋白(Tf)基因序列,先设计并合成扩增引物扩增出大黄鱼转铁蛋白部分基因序列,再应用RACE技术,克隆出大黄鱼转铁蛋白全长cDNA基因,全长2461个核苷酸,编码661个氨基酸。应用WCG软件包,采用DNADIST和NEIGHBOR分析方法,构建了转铁蛋白系统进化树。树图显示与传统的分类地位大致相符,可以看出转铁蛋白在海水鱼和淡水鱼的进化上的差异,同时也显示大黄鱼Tf与真鲷的同源性最高。     

南海南部晚中新世的放射虫及其环境探讨

南海是东亚古季风产物的主要沉积盆地，保存着比陆地更加完整和连续的沉积记录。详细分析了ODP1143号钻井晚中新世的放射虫化石群，主要根据标志种Diartus petterssoni,D.hughesi和Stichocorys delmontensis等的分布特征，分别建立了南海南部晚中新世的RN6，RN7和RN8等3个放射虫化石带，并讨论了其地层年龄。探讨了以地层中放射虫的丰度变化特征等在南海南部作为东亚古夏季风活动替代性指标的可能性，初步说明东亚古夏季风可能早于8．7Ma B.P．出现，约在8．24Ma B.P.强化达到高峰，认为与印度季风的出现几乎同步或略早。     

Seasonal variation of global surface pressure and water vapor

Previous studies have shown that the seasonal variation of global-mean surface pressure ( p _s) results from variation of global-mean water vapor pressure ( p _w). The current study, employing the global data generated by Version 1 of the Goddard Earth Observing System (GEOS-1) Data Assimilation System, shows that seasonal variations of regional p _s and p _w tend to be out of phase (particularly in the subtropics of the two hemispheres) and that the magnitude of the former variation is generally much larger than that of the latter. The seasonal variations of these two quantities are maintained by airmass and water vapor transports by the global divergent circulation, which is driven by the latent heat released by cumulus convection over the water vapor sink, as the "water mass forcing" mechanism predicted. Since p _w and p _s are used often in depicting the climate system, assessments of climate change in terms of the global-mean and regional variations of these two variables should be interpreted with caution.     

RADIOLARIA IN CORES FROM THE OKINAWA TROUGH

A total of 149 genus and 238 species of Radiolaria in cores from four stations in the Okinawa Trough are examined in this paper. Water depths and cores lengths at these stations are 602-1900 m and 1.7-2.4 m respectively (see Fig. 1). Based on the distributional characteristic of Radiolaria and the radio-larian temperature number (Tr), the climatic fluctuations since the last 150 000 years and the stratigra-phic classification are also discussed.     

基于神经网络的平底结构砰击压力预报

对利用神经网络预报平底结构入水砰击压力的方法进行了探讨。首先利用仿真软件计算各种情况下平底结构入水所产生的砰击压力，以此形成训练神经网络的数据集。其次利用数据集对三层反馈式网络进行了训练，讨论了不同隐含层节点数对该非线性系统的拟合能力，并且对梯度下降法、动量修正法和基于优化的LM算法的有效性和精度进行了比较，最后得出了适合平底结构入水砰击系统的网络结构。     

健康型牛蛙饲料养殖牛蛙的试验

本研究采用5种中草药添加剂配方的膨化饲料进行了为期36d的牛蛙养殖对比试验.结果表明，饲料中添加有中草药制剂的日摄食量明显大于对照组，日增重显著比对照组的高.而且大大加快了生长速度，缩短养殖周期，同时减少了染病机率，牛蛙体色鲜艳、光泽度极佳.