沿海地区胃脘疼痛的中药外治法

沿海地区胃脘疼痛的中药外治法山东省青岛医学院中医教研室（２６６０１２）张玉杰安培祯主题词：胃脘疼痛／外治法中药／治疗应用中医学是将人体与外在环境密切联系并结合实践，以因人、因地、因时制宜来作为中医治疗学的重要依据。我们地处沿海，湿性重浊，极易侵袭脾胃...     

54例农村新生儿住院病例分析

我院1988年2月至1993年3月共收治新生儿患儿261例。在此对来自农村的新生儿进行了回顾性分析，显示农村围产医疗保健存在不足，临床资料54例患儿中，男29例，女25例，均为足月儿，低体重儿3例。因病情危重放弃治疗6例，死亡7例，占住院新生儿死亡率的70％。54例病儿中，肺炎18例（33．3％），脐炎7例，（12．9％），缺氧缺血性脑病7例（12．9％），硬肿症13例（24．07％），窒息5例（9．26％），其它4例。1．分娩史回顾。本组病儿中非自然分娩19例均未做过产前检查。在家分娩21例，有17例发生不同程度窒息。死亡7例中，在家分娩的占5例。2．…     

自制螺杆外固定架逐渐加压治疗陈旧性Tile B1型骨盆骨折

目的探讨应用自制螺杆外固定架逐渐加压治疗陈旧性TileB1型骨盆骨折的疗效。方法自2005年2月～2008年6月,收治陈旧性TileB1型骨盆骨折患者12例,均采用自制螺杆外固定架逐渐加压治疗,术后8周～6个月拆除外固定架。结果术后12例均获随访,随访时间6～8个月,平均13个月,临床疗效满意。术后6个月骨盆平片示耻骨联合间距与术前间距比较,差异有统计学意义（P〈0.05）,疗效参照Majeed评价标准,优4例,良3例,中3例,可2例,总体优良率58.33%。结论应用自制螺杆外固定架逐渐加压治疗陈旧性TileB1型骨盆骨折是一种有效且操作简单微创的方法。     

我国居民基层医疗机构首诊意愿的Meta分析

目的 系统评价我国居民基层医疗机构首诊意愿率.方法 计算机检索CNKI、WanFang Data、VIP、PubMed、Web of Science和EMbase数据库,搜集有关居民基层医疗机构首诊意愿率的横断面研究,检索时限为2006年1月至2020年11月.由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险...     

何首乌水提物对大鼠肝脏CYP1A2,CYP2E1酶活性及mRNA表达抑制作用研究

大鼠连续灌胃不同剂量的何首乌水提物(1,10g·kg-1)7 d,取肝脏制备肝微粒体,分别用Cocktail体外孵育法和实时荧光定量PCR技术观察何首乌水提物对大鼠肝脏主要CYP450酶活性及mRNA表达的影响.与空白对照组相比,1,10g·kg-1何首乌水提物组大鼠肝脏CYP2E1酶活性和mRNA的表达均受到明显抑制(CYP2E1酶活性,P＜0.01;CYP2E1的mRNA表达1 g·kg-1组P＜0.05,10 g·kg-1组P＜0.01),CYP3A1酶活性虽显示明显升高(P＜0.01),但mRNA的表达没有显著变化;10 g·kg-1何首乌水提物组大鼠肝脏CYP1A2酶活性和mRNA的表达受到明显抑制(P＜0.01).     

蚌埠市某医院31533例健康体检者贫血情况分析

目的 对安徽省蚌埠地区2017-2019年在该院体检的人群进行贫血调查,以期为贫血的防治及健康管理提供理论支持.方法 选取2017-2019年在该院进行体检的31533例健康体检者作为研究对象,分别以年龄和性别进行分组,对各组贫血情况进行总结.结果 31533例体检者中共检出贫血患者999例,检出率为3.17％,其中男...     

腰扭伤的针灸疗法

近几年来，用针炙治疗腰扭伤，总结出了许多新颖的治疗方法，对穴位的应用有诸多创新。手部闪腰穴、后溪穴，眼部的睛明穴、攒竹穴、印堂穴，耳穴的神门、皮质下、肾上腺、腰椎、腰痛点、热穴，以及百会、天柱、大椎、腰宁、条口、附阳穴，其有效率均达９５％以上；药物及空气穴位注射的有效率达１００％。提示针灸治疗腰扭伤有较好的疗效。     

光疗治疗银屑病的研究进展

目前治疗银屑病的方法较多,但治疗效果令人满意的方法尚少。光疗是治疗银屑病的一种有效方法,与药物联合应用不仅可以提高疗效,还可减少不良反应的发生。现将近年光疗治疗银屑病的研究进展综述如下。     

Expression of type Ⅰ interferon in monocyte-derived dendritic cells after Toll like receptor 3 triggered in patients with chronic hepatitis B

Objective To detect the expression of type Ⅰ interferon in monocyte-derived dendritic cells(MoDCs)after Toll like receptor(TLR)3 triggered in patients with chronic hepatitis B(CHB),and to evaluate immune responses of CHB patients and its roles in the mechanisms of persistent infection of hepatitis B virus(HBV)and chronicity of hepatitis.Methods Peripheral blood mononuclear cells(PBMCs)were isolated and purified using magnetic beads(plasma was saved simultaneously)from 26 CHB patients and 18 healthy volunteers(HV).Dendritic cells(DCs)were induced and proliferated in a culture medium with recombinant human granulocyte macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin(rhIL-4).EX3s were stimulated with Poly Ⅰ:C and the supernatants were collected at 0 h and 24 h after stimulation.Type Ⅰ interferon(IFN-α and IFN-β)in plasma and supernatants were examined by enzyme linked immunosorbent assay (ELISA).Results The levels of type Ⅰ interferon in plasma were not significantly different in groups of HV and CH B.IFN-α and IFN-β expressions in supernatants before Poly Ⅰ:C stimulation were(80.00±16.15)ng/L,(36.39±13.90)ng/L in CHB group and(76.76±15.90)ng/L,(37.14±13.68)ng/L in HV group,respectively.And there were no statistical differences between two groups(t=1.651,t=0.178;both P＞0.05).IFN-α expressions in supernatants at 24 h after stimulation in two groups were both higher than those before stimulation(at 0 h),but there were no statistical differences(t=1.534,t=1.243;both P＞0.05).IFN-β expressions in supernatants at 24 h after stimulation in HV group was(54.57±16.80)ng/L,which was significantly higher than that at 0 h(37.14±13.68)ng/L(t=4.061,P＜0.05).However,there was no significant difference at 24 h than tht at 0 h in CHB group(t=1.796,P＞0.05).At 24 h after stimulation.IFN-β level was(54.57±16.80)ng/L in HV group,which was significantly higher than that[(41.64±12.57)ng/L]in CHB group(t=2.921,P＜0.05).Conclusions Functions of MoDCs from CHB patients are impaired and MoDCs could not express type Ⅰ interferon normally.Expression of type Ⅰ interferon after TLR3 triggered in CHB patients is mainly IFN-β.     

Expression of type Ⅰ interferon in monocyte-derived dendritic cells after Toll like receptor 3 triggered in patients with chronic hepatitis B

Objective To detect the expression of type Ⅰ interferon in monocyte-derived dendritic cells(MoDCs)after Toll like receptor(TLR)3 triggered in patients with chronic hepatitis B(CHB),and to evaluate immune responses of CHB patients and its roles in the mechanisms of persistent infection of hepatitis B virus(HBV)and chronicity of hepatitis.Methods Peripheral blood mononuclear cells(PBMCs)were isolated and purified using magnetic beads(plasma was saved simultaneously)from 26 CHB patients and 18 healthy volunteers(HV).Dendritic cells(DCs)were induced and proliferated in a culture medium with recombinant human granulocyte macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin(rhIL-4).EX3s were stimulated with Poly Ⅰ:C and the supernatants were collected at 0 h and 24 h after stimulation.Type Ⅰ interferon(IFN-α and IFN-β)in plasma and supernatants were examined by enzyme linked immunosorbent assay (ELISA).Results The levels of type Ⅰ interferon in plasma were not significantly different in groups of HV and CH B.IFN-α and IFN-β expressions in supernatants before Poly Ⅰ:C stimulation were(80.00±16.15)ng/L,(36.39±13.90)ng/L in CHB group and(76.76±15.90)ng/L,(37.14±13.68)ng/L in HV group,respectively.And there were no statistical differences between two groups(t=1.651,t=0.178;both P＞0.05).IFN-α expressions in supernatants at 24 h after stimulation in two groups were both higher than those before stimulation(at 0 h),but there were no statistical differences(t=1.534,t=1.243;both P＞0.05).IFN-β expressions in supernatants at 24 h after stimulation in HV group was(54.57±16.80)ng/L,which was significantly higher than that at 0 h(37.14±13.68)ng/L(t=4.061,P＜0.05).However,there was no significant difference at 24 h than tht at 0 h in CHB group(t=1.796,P＞0.05).At 24 h after stimulation.IFN-β level was(54.57±16.80)ng/L in HV group,which was significantly higher than that[(41.64±12.57)ng/L]in CHB group(t=2.921,P＜0.05).Conclusions Functions of MoDCs from CHB patients are impaired and MoDCs could not express type Ⅰ interferon normally.Expression of type Ⅰ interferon after TLR3 triggered in CHB patients is mainly IFN-β.