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1.
Michael J. A. Young Arlo W. Fast Paul G. Olin 《Journal of the World Aquaculture Society》1989,20(1):7-11
Chinese catfish ( Clarias fuscus ) were successfully spawned in Hawaii using human chorionic gonadotropin (HCG) at dosage rates of two and four international units (IU) per gram body weight. Fish not injected with HCG did not produce viable eggs. Successful spawns with HCG occurred between May and October. Hatch rates of up to 80% were obtained during June, July, and August for those fish given either a 2 or 4 IU per gram body weight injection of HCG. Fish spawned in either May or October yielded significantly higher hatch rates when injected with 4 rather than 2 IU per gram body weight. Fish held at elevated temperatures (28 to 30 C) prior to the normal spawning season developed significantly larger oocyte diameters, 60 days earlier than fish held under ambient temperature conditions (21.5 to 24 C). Photoperiod manipulation at ambient temperature conditions was associated with earlier oocyte maturation, but photoperiod effects were much less important than temperature. 相似文献
2.
用两株单克隆抗体混合后包被ELLISA微孔板,加牛冠状病毒抗原,加经白陶土处理过的兔抗牛冠状病毒免血清,再加上辣根氧化物酶标记的羊抗兔IgG抗体,加底物质显色间接ELISA检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液,其敏感度高出血凝试验(HA)8倍,从武汉市附近3个奶牛场采集犊牛腹泻粪便105份,用单抗ELISA检测牛冠状病毒抗原,共检出阳性36份,并与血凝及血凝抑制试验, 相似文献
3.
4.
I.-S. Park G. C. Choi D. S. Kim Y. K. Nam 《Journal of the World Aquaculture Society》2002,33(4):494-500
For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response of mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 ± g and sperm density was estimated by a standard hemocytomer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y = 3.68X - 27.18 ( R 2 = 0.82, N = 50). where Y is spermatocrit and × is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 μg luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermatocrit and sperm density levels. These results demonstrate that spermiation in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effective in stimulating of spermiation in this species. 相似文献
5.
试验结果表明谷子在—13.9Pa的负压下发芽率只有24.0%,在—16.73Pa时发芽率为16.3%。用0.5%的NM—1号抗旱剂包衣后,在—16.73Pa的负压下发芽率可达65.6%,比对照提高49.3%。同0.5%的NM—1抗旱剂在大豆现蕾期喷施叶面1次,相对收获株率为115.38%,相对千粒量为107.7%,增产率为40,27%。玉米在6叶期喷施0.5%的抗旱剂后,饱和亏减少4.09g,是对照的43.81%;干物质积累比对照快67.19%,蒸腾系数减少8.36g。 相似文献
6.
Jamie S. Harmon Kim G. Michelsen Dr. Mark A. Sheridan 《Fish physiology and biochemistry》1991,9(4):361-368
Trialcylglycerol (TG) lipase was isolated and partially purified from rainbow trout liver. Triacylglycerol lipase activity was assayed by measuring14C-oleic acid release from14C-triolein.14C-oleic acid release was linear for up to two hours. Optimal activity occurred at pH 7.0 and 15°C. Most of the lipase activity was recovered in the cytosolic fraction. A 27,000-fold purification was achieved after Sepharose (Bio-gel A 0.5 M, 200–400 mesh) chromatography of a resuspended 20% ammonium sulfate fraction. The molecular weight of the trout hepatic lipase as determined by size-exclusion chromatography and by SDS-polyacrylamide gel electrophoresis was 40–43 kD. Lipase-mediated hydrolysis of TG resulted in the production of diacylglycerols, monoacylglycerols, and fatty acids. Kinetic analysis indicated that Vmax=0.016 nmol/h/mg protein and that Km=0.28 mM triolein. Lipolytic activity was enhanced in the presence of cAMP/ATP-Mg2+. These results suggest that the liver of trout possesses a neutral TG lipase that is responsible for mobilizing stored TG and is catalytically activated by phosphorylation.A part of this work was presented at the Annual Meeting of the American Society of Zoologists, December 26–30, 1990, San Antonio, TX. 相似文献
7.
压力实时监测法在管道泄漏检测中的应用 总被引:2,自引:0,他引:2
检测输油管道漏油的方法很多,介绍了通过压力实时监测确定输油管道漏油点方法,以及实际应用情况。实践证明,通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。 相似文献
8.
9.
Hemagglutination and hemagglutination inhibition of turkey red blood cells with Mycoplasma hyopneumoniae 总被引:2,自引:0,他引:2
T F Young B Z Erickson R F Ross Y Wannemuehler 《American journal of veterinary research》1989,50(7):1052-1055
The ability of Mycoplasma hyopneumoniae to agglutinate RBC was evaluated to develop an in vitro cytadsorption assay. Using swine RBC in a microtitration hemagglutination test, no agglutination or partial agglutination was detected. Comparison of RBC from various other species indicated that improved hemagglutination was obtained with RBC from turkeys. This hemagglutination was detected only when mycoplasma cells used in the assay had been frozen and thawed, heated at 50 C for 30 minutes, or treated with trypsin. Treatment of RBC with trypsin or neuraminidase enhanced hemagglutination. Possible surface lectin activity in M hyopneumoniae was evaluated by use of carbohydrates in a blocking assay; hemagglutination was not inhibited by any of 13 carbohydrates evaluated. Mycoplasma hyopneumoniae convalescent porcine serum and monoclonal antibodies against 2 M hyopneumoniae immunogens of molecular weights of 64,000 and 41,000 inhibited hemagglutination. 相似文献
10.