Enzymatic degradation of thermoresponsive poly(N‐isopropylacrylamide) grafted to carboxymethylcellulose copolymers

The enzymatic degradation of poly(N‐isopropyl acrylamide) (PNIPAM) grafted to carboxymethylcellulose (CMC) copolymers with a cellulasic preparation (Trichoderma viride) was studied. The enzymatic activity of the cellulasic preparation against CMC and the grafted copolymers was determined by the Petterson–Porath method, while their reduced viscosity variation in the presence of the same preparation was also followed. It has been shown that the enzymatic degradation behavior depends on the copolymer composition and the reaction temperature. Reducing sugars analysis showed that the experimental values for the grafted copolymers were higher than the calculated ones. At 50°C, the enzymatic reaction is completed in about 20 min for the copolymers, whereas for CMC it takes more than 40 min. It can be concluded that their enzymatic degradation is facilitated by the presence of the PNIPAM grafts. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 87: 1383–1386, 2003     

Compatibilisation of PPE/SAN blends by triblock terpolymers: Correlation between block terpolymer composition, morphology and properties

Immiscible blends of poly(2,6-dimethyl-1,4-phenylene ether) (PPE) and poly(styrene-co-acrylonitrile) (SAN) with a weight composition of 60/40 were compatibilised by polystyrene-block-polybutadiene-block-poly(methyl methacrylate) triblock terpolymers (SBM) using a two-stage melt-processing approach. In order to investigate the influence of the SBM composition on the compatibilisation efficiency, the block lengths of the triblock terpolymers were systematically varied. The resulting morphological features of the blend systems as function of SBM composition and processing parameters are correlated with the resulting thermal and thermo-mechanical properties. In the ideal case, SBM should be located at the interface as PS is miscible with PPE while PMMA is miscible with SAN. The elastomeric middle block as an immiscible component should remain at the interface. This particular morphological arrangement is known as the ‘raspberry morphology’. A detailed TEM analysis of the blend morphologies following initial extrusion-compounding revealed a high compatibilisation efficiency of the SBM types with equal lengths of the end blocks and, furthermore, the desired raspberry morphology was achieved. In contrast, high PS contents in comparison to the other blocks led to a pronounced micelle formation in the PPE phase. Further evaluation of the blend structures following injection-moulding indicated that the morphologies remain relatively stable during this second melt-processing step. A detailed thermal analysis of all blend systems supports the interpretation of the observed morphological features. The fundamental correlation between SBM composition and blend morphology established in this study opens the door for the controlled development of interfacial properties of such compatibilised PPE/SAN blends during melt-processing.     

Towards long term monitoring of electrodermal activity in daily life

Manic depression, also known as bipolar disorder, is a common and severe form of mental disorder. The European research project MONARCA aims at developing and validating mobile technologies for multi-parametric, long term monitoring of physiological and behavioral information relevant to bipolar disorder. One aspect of MONARCA is to investigate the long term monitoring of Electrodermal activity (EDA) to support the diagnosis and treatment of bipolar disorder patients. EDA is known as an indicator of the emotional state and the stress level of a person. To realize a long-term monitoring of the EDA, the integration of the sensor system in the shoe or sock is a promising approach. This paper presents a first step towards such a sensor system. In a feasibility study including 8 subjects, we investigate the correlation between EDA measurements at the fingers, which is the most established sensing site, with measurements of the EDA at the feet. The results indicate that 88% of the evoked skin conductance responses (SCRs) occur at both sensing sites. When using an action movie as psychophysiologically activating stimulus, we have found weaker reactivity in the foot than in the hand EDA. The results also suggest that the influence of moderate physical activity on EDA measurements is low and has a similar effect for both recording sites. This suggests that the foot recording location is suitable for recordings in daily life even in the presence of moderate movement.     

Flank milling with flat end milling cutters

One situation encountered in industry is that two curves on the machined surface are known, such as the top and bottom profiles of a blade. The shape of the surface is not known and is to be determined by a tool sliding along the generating rails. In this paper, we give a detailed mathematical understanding of flank milling with flat end cutters, which we then use to develop a method for milling with such a cutter. This method slides the cutter along two rails, keeping the cutter tangent to both curves at every parameter value. Examples are given to illustrate the method, along with simulations and error analysis.     

Phytochemical composition and antioxidant capacity of various botanical parts of the fruits of Prunus × domestica L. from the Lorraine region of Europe

Mirabelle plums represent a famous fruit from the Lorraine region, however little is known about their phytochemical composition. The oil of the fruit contained predominantly oleic acid (59%) and linoleic acid (29%). The total content of phenolic antioxidants in the whole fruits was 5.338 g/kg with 456 mg/kg (9%), 701 mg/kg (13%) and 4159 mg/kg (78%) detected in the peels, flesh and pits respectively. The peels contained solely 3,4-dihydroxybenzoic acid (270 mg/kg) and rutin (186 mg/kg), the flesh exclusively echinoids (723 mg/kg), whereas the pits contained a rich variety of phenolic compounds (4.2 g/kg) dominated by amygdalin (3.8 g/kg), but with significant contributions from vanillin (102 mg/kg), guajacyl-glycerin-coniferyl aldehyde isomers (87 mg/kg), dehydro-diconiferyl aldehyde (52 mg/kg), and vanillin diglucoside (48 mg/kg). Of the major phenolic compounds tested across a range of in vitro assays, rutin was the superior antioxidant.     

Konduktometrische und spektroskopische Untersuchungen der Wechselwirkung von Lewis-Säuren mit Halogenkomplexen

Conductometric and Spectroscopic Investigations of the Interaction of Lewis Acids with Halide Complexes The interaction of counter ions with Lewis acids has been investigated using mode systems of the type (C₆H₅)₃C^⊕ MX_n+1^⊖ (M = Sb, As, B, Fe; X = Cl, F) by means of conductometrical and UV-spectroscopic methods. The changes of conductivity were strongly dependent on the nature and concentration of the Lewis acid. The results point to 1:1-complexes between Lewis acids and counter ions. The behaviour was found to be complicated by dissoziation resp. assoziation processes. Ligand exchange reactions between Lewis acid and counter ion have also been found.     

Adaptation of Oxidative Phosphorylation Machinery Compensates for Hepatic Lipotoxicity in Early Stages of MAFLD

Alterations in mitochondrial function are an important control variable in the progression of metabolic dysfunction-associated fatty liver disease (MAFLD), while also noted by increased de novo lipogenesis (DNL) and hepatic insulin resistance. We hypothesized that the organization and function of a mitochondrial electron transport chain (ETC) in this pathologic condition is a consequence of shifted substrate availability. We addressed this question using a transgenic mouse model with increased hepatic insulin resistance and DNL due to constitutively active human SREBP-1c. The abundance of ETC complex subunits and components of key metabolic pathways are regulated in the liver of these animals. Further omics approaches combined with functional assays in isolated liver mitochondria and primary hepatocytes revealed that the SREBP-1c-forced fatty liver induced a substrate limitation for oxidative phosphorylation, inducing enhanced complex II activity. The observed increased expression of mitochondrial genes may have indicated a counteraction. In conclusion, a shift of available substrates directed toward activated DNL results in increased electron flows, mainly through complex II, to compensate for the increased energy demand of the cell. The reorganization of key compounds in energy metabolism observed in the SREBP-1c animal model might explain the initial increase in mitochondrial function observed in the early stages of human MAFLD.     

Structure from Motion: Beyond the Epipolar Constraint

The classic approach to structure from motion entails a clear separation between motion estimation and structure estimation and between two-dimensional (2D) and three-dimensional (3D) information. For the recovery of the rigid transformation between different views only 2D image measurements are used. To have available enough information, most existing techniques are based on the intermediate computation of optical flow which, however, poses a problem at the locations of depth discontinuities. If we knew where depth discontinuities were, we could (using a multitude of approaches based on smoothness constraints) accurately estimate flow values for image patches corresponding to smooth scene patches; but to know the discontinuities requires solving the structure from motion problem first. This paper introduces a novel approach to structure from motion which addresses the processes of smoothing, 3D motion and structure estimation in a synergistic manner. It provides an algorithm for estimating the transformation between two views obtained by either a calibrated or uncalibrated camera. The results of the estimation are then utilized to perform a reconstruction of the scene from a short sequence of images.The technique is based on constraints on image derivatives which involve the 3D motion and shape of the scene, leading to a geometric and statistical estimation problem. The interaction between 3D motion and shape allows us to estimate the 3D motion while at the same time segmenting the scene. If we use a wrong 3D motion estimate to compute depth, we obtain a distorted version of the depth function. The distortion, however, is such that the worse the motion estimate, the more likely we are to obtain depth estimates that vary locally more than the correct ones. Since local variability of depth is due either to the existence of a discontinuity or to a wrong 3D motion estimate, being able to differentiate between these two cases provides the correct motion, which yields the least varying estimated depth as well as the image locations of scene discontinuities. We analyze the new constraints, show their relationship to the minimization of the epipolar constraint, and present experimental results using real image sequences that indicate the robustness of the method.