海上油井密封套管内海水冻胀特性试验分析

1998年首次进行了海上油井密封套管内海水冻胀特性模拟试验,建立了一套各种参数的测量系统.文中介绍了管内海水冻结过程、海水过冷温度、结冰水温、轴向冻胀力以及管体应变测量结果.     

南极布兰斯菲尔德海峡表层沉积物的酸解烃

本文研究了南极布兰斯菲尔德海峡表层沉积物的酸解烃分布特征，并佐以热释汞和蚀变碳酸盐的分析。从酸解烃中甲烷含量的低值和Ｃ１／（Ｃ２＋Ｃ３）比值，以及乙烯的存在等判断，布兰斯菲尔德海峡沉积物的烃类气体来自于生物成因。     

Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

Identification of phase and sex-related ISSR markers of red algaGracilaria lemaneiformis

Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 and SF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank.     

Three‐dimensional shakedown solutions for anisotropic cohesive‐frictional materials under moving surface loads

Previous work on three‐dimensional shakedown analysis of cohesive‐frictional materials under moving surface loads has been entirely for isotropic materials. As a result, the effects of anisotropy, both elastic and plastic, of soil and pavement materials are ignored. This paper will, for the first time, develop three‐dimensional shakedown solutions to allow for the variation of elastic and plastic material properties with direction. Melan's lower‐bound shakedown theorem is used to derive shakedown solutions. In particular, a generalised, anisotropic Mohr–Coulomb yield criterion and cross‐anisotropic elastic stress fields are utilised to develop anisotropic shakedown solutions. It is found that shakedown solutions for anisotropic materials are dominated by Young's modulus ratio for the cases of subsurface failure and by shear modulus ratio for the cases of surface failure. Plastic anisotropy is mainly controlled by material cohesion ratio, the rise of which increases the shakedown limit until a maximum value is reached. The anisotropic shakedown limit varies with frictional coefficient, and the peak value may not occur for the case of normal loading only. Copyright © 2013 John Wiley & Sons, Ltd.     

断层EDF技术在复杂断块构造研究中的应用

复杂断块构造具有断层复杂,小断层广泛发育,断层识别难度大,储层分布及连通性复杂,地震成像不清、资料品质较差等特征。对于此类问题,常规地震资料无法满足断层解释精度要求。提出了一种新的断层EDF解释性处理技术,将地震资料处理与解释融为一体,以提高资料的信噪比和断层的识别能力。断层EDF解释性处理技术是利用扩散滤波和中值倾角滤波相结合的断层增强滤波方法来提高资料的信噪比,突出断层和地层的接触关系,再在滤波后的地震资料数据体上进行分频处理,选择最佳成像频带,突出识别不同规模的断层。通过在涠洲A油田的应用,取得了良好的效果。     

浅论县（市）级土地利用数据库系统建设

随着经济的迅猛发展,各地土地利用情况多种多样,采用手工管理土地资料更新周期长,综合利用率低,各种查询统计要耗费大量的人力物力,而且统计计算的数据结果精度不高。因此仅仅依靠传统的手工管理模式已经远远不能满足土地管理工作的需要,为保质保量按时完成全国土地利用现状更新调查,加强地籍规范化管理,利用成熟的计算机技术辅助进行土地调查已势在必行。     

应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)

参照鱼类病毒性出血性败血症病毒序列,根据PCR引物设计的原则,设计巢式PCR引物,采用巢式逆转录聚合酶链反应(RT-PCR)方法,对如何快速检测鱼类病毒性出血性败血症病的方法进行了较为系统的研究,并对RT-PCR的反应条件进行了优化.结果表明,巢式RT-PCR扩增获得279bp的特异性片断,阴性对照无扩增条带.巢式RT-PCR扩增出的特异性片段经测序分析,结果证实与报道的序列完全一致.该方法最低可检测出0.1pg的鱼类病毒性出血性败血症病毒RNA.初步建立了VHSV的巢式RT-PCR检测方法,该方法灵敏、特异,可为VHSV的检测提供一个快速、有效的手段.     

SPOT图象的解析立体测图

本文讨论了适合于SPOT图象的用光束法平差解算外方位元素的数学模型;介绍了在国产解析测图仪APS—1上进行SOPT图象立体测图的方法;研究了SPOT投影方式的实时循环程序LOOP及SPOT图象的测图软件包。