内蒙古贺根山地区早二叠世二长花岗岩时代、成因及构造意义

贺根山地区位于华北板块和西伯利亚板块的结合部位。为确定二长花岗岩形成时代与构造背景,开展锆石U-Pb年代学和地球化学研究。结果表明,锆石U-Pb加权平均年龄为(284.6±1.3)Ma,为早二叠世岩浆活动产物。二长花岗岩SiO_2含量70.11%~71.06%,Al_2O_3含量14.94%~15.51%,明显贫镁铁,全碱含量ALK为6.90%~7.22%,Na_2O/K_2O值为3.57~4.17,A/CNK值为1.28~1.34,为过铝质钠质钙碱性系列。稀土分布曲线呈右倾轻稀土富集型,负铕异常明显,δEu=0.47~0.49;富集大离子亲石元素Rb,Ba,U,亏损高场强元素Nb,Ta,Th,Ti;属A2型花岗岩。结合区域资料推断,二长花岗岩形成于造山后伸展环境。贺根山缝合带在早二叠世之前形成,晚石炭世贺根山蛇绿岩残片完成构造就位,贺根山洋陆续闭合,暗示古亚洲洋至少在早二叠世之前已闭合。     

山西省胡家峪南和沟铜矿床深部矿体定位预测的原生晕指示

胡家峪南和沟铜矿床是山西省中条山铜矿集中区胡篦型矿田的一个中型矿床,已被列为国家危机矿山.本文选择化探原生晕找矿方法对该矿山深部矿体开展成矿预测工作,以期为矿山下一步探矿工作安排提供科学依据.     

Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification

Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65°C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.     

Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis

INTRODUCTIONTriploidanimalsareusefulbecausetheyaresterile ,characterizedbypositivegrowthinthere productiveseason ,usefulfortesting ,forintroductionofnon nativespecies ,andforprotectionofde velopedstrains.Theinductionoftriploidyhadbeenreportedinmanyaquaculturespecies .Triploidshavebeensuccessfullyinducedinshrimpusingtemperatureshockorchemicalshock (CB ,6 DMAP)in 4species :Sicyoniaingentis (Xiangetal.,1 991 ) ,Fenneropenaeuschinensis (Xiangetal.,1 992 ;Daietal.,1 993;Baoetal.,1 993;Li…     

内蒙古崇根山蛇绿岩前弧玄武岩的发现及其地质意义

内蒙古崇根山SSZ型蛇绿岩位于兴蒙造山带北部的二连浩特－贺根山蛇绿岩带,为贺根山蛇绿岩中出露面积最大、岩性出露最为齐全的蛇绿岩块,主要组成端元为橄榄岩,少量堆晶辉长岩和拉斑玄武岩片.研究表明,该玄武岩为 一 套 前 弧 玄 武 岩,岩 性 为 低 钾 拉 斑 玄 武 岩.岩 石 w(SiO２)为 ４８．５８％ ~５１．２３％,含 较 低 的 TiO２
(１．６３％~２．０８％),富 Na２O (３．１３％~４．７３％)而贫 K２O(０．０５％~０．２０％),Na２O/K２O 为２３．７~７９．０;岩石具有亏损型稀土元素配分模式,类似 NＧMORB,微量元素富集 Rb、Ba、U、Ta、Hf,亏损 Th、Nb、Sr,元素质量分数普遍略高于 NＧMORB,与弧拉斑玄武岩相似.地球化学特征上兼具洋中脊与岛弧双重特点,且更偏向洋中脊,与马里亚纳前弧玄武岩(FAB)相一致.综合分析前人对于崇根山蛇绿岩块及区域蛇绿岩同位素年代学研究成果表明,二连浩特－贺根山蛇绿岩带在早石炭世存在前弧玄武岩,说明二连浩特－贺根山洋盆在早石炭世发生了洋内初始俯冲和洋陆转化岩浆作用.      

刀额新对虾染色体核型及细胞核DNA含量

以刀额新对虾胚胎、无节幼体、卵巢、精巢等为材料 ,空气干燥法制备染色体 ,并初步进行核型分析。结果表明 ,刀额新对虾的染色体数目为 2n=78,n =39,核型为 2n=78=40M 1 0SM 1 4ST 1 4T。以刀额新对虾血淋巴、卵巢、肌肉、鳃为材料 ,以鸡血细胞为DNA标准 (2 50pg/ 2c) ,使用PartecCCAⅡ型流式细胞仪测定了刀额新对虾细胞的基因组DNA含量 ,其值为鸡血细胞的 1 75倍 ,绝对含量为 4 375pg/ 2c。     

混合所有制的探索与实践

正南昌有色冶金设计研究院(以下简称"南昌院")成立于1957年,先后隶属于冶金工业部、中国有色金属工业总公司、国家有色金属工业局,2000年下放江西省实行属地化管理,为省政府直属正厅级企业化管理的事业单位,2004年被列为省国资委出资监管企业。2007年,经江西省政府批准,南昌院正式改制为中国瑞林工程技术有限公司(以下简称"中国瑞     

盾构隧道掘进全过程三维模拟方法及重叠段近接分区研究

合理地模拟盾构隧道掘进的全过程及盾构隧道重叠段近接分区是一个难点。依托深圳地铁3号线红岭中路－老街－晒布段区间重叠隧道工程进行研究,建立了能全面反映盾构隧道掘进全过程的三维模拟方法,并采用摩尔-库仑屈服准则对盾构隧道重叠段进行了横向近接分区,同时采用位移变化速率准则对盾构隧道重叠段进行了纵向近接分区。研究结果表明,盾构隧道重叠段横向近接分区为：F≤F? 时为无影响区,F?＜F＜0时为弱影响区,当F≥0时为强影响区;纵向近接分区为：小于0.3 mm/d为无影响区,0.3～1.5 mm/d为弱影响区,大于1.5 mm/d为强影响区。研究成果应用于红岭中路－老街－晒布段区间重叠隧道取得了良好的效果,并为盾构隧道掘进全过程的数值模拟以及盾构隧道重叠段区间隧道的设计与施工提供了理论支撑,同时亦可为相似工程提供参考。     

四川天宝山矿床闪锌矿Rb-Sr年代学、稳定同位素及地质意义

四川天宝山铅锌矿床是赋存于震旦系灯影组白云岩中的大型铅锌矿床,一直以来缺乏精确的成矿年龄数据,致使其成矿构造环境存在争议.获取了闪锌矿Rb-Sr年龄,联合H-O、C-O同位素数据,以确定其成矿年代、成矿物质来源及成矿构造环境.闪锌矿Rb-Sr年龄为348.5±7.2 Ma(MSWD=1.10),表明矿床形成于早石炭世.热液矿物δD_H2O、δ18O值分别为-19.3‰~-58.1‰、-1.4‰~0.6‰,沿海水与地层有机质反应线分布,并有向雨水线漂移的趋势,说明成矿流体中水是海水与地层有机质反应并加入雨水的混合体.热液方解石δ13C、δ18O值明显分为两群,分别为-1.7‰~-1.6‰、12.9‰~15.2‰和-6.5‰~-4.9‰、19.3‰~20.2‰,暗示成矿流体中C、O可能来源于赋矿围岩溶解作用和有机质脱羧基作用.闪锌矿(87Sr/86Sr)_i值为0.710 42 ±0.000 13,高于赋矿围岩Sr同位素值而明显低于基底Sr同位素值,指示成矿物质主要来源于赋矿围岩与基底.结果表明川滇黔地区存在两期铅锌成矿作用,分别形成于晚泥盆世-早石炭世与古特提斯洋张开有关的伸展构造环境和晚三叠世-早侏罗世与古特提斯洋闭合有关的收缩构造环境.      

Construction of bacterial artificial chromosome libraries for Zhikong Scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis>

Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.