Intercolony variability of skeletal oxygen and carbon isotope signatures of cultured Porites corals: Temperature-controlled experiments

The skeletal oxygen isotope ratio of Porites corals is the most frequently used proxy of past seawater temperature and composition for tropical and subtropical oceans. However, field calibration of the proxy signals is often difficult owing to the dual dependence of skeletal oxygen isotope ratio on temperature and the oxygen isotope composition of water. We conducted tank experiments in which we grew Porites spp. colonies for 142 d in thermostated seawater at five temperature settings between 21°C and 29°C under moderate light intensity of 250 μmol m⁻² s⁻¹ with a 12:12 light:dark photoperiod. A skeletal isotope microprofiling technique applied along the major growth axis of each colony revealed that the oxygen isotope ratios of newly deposited skeleton in most colonies remained almost constant during tank incubation, thus providing an ideal situation for precise calibration of oxygen isotope ratio proxy signals. However, the oxygen isotope ratios displayed an unusually large intercolony variability (∼1‰) at each temperature setting although the mean slope (∼0.15‰ °C⁻¹) obtained for the temperature-skeletal oxygen isotope ratio relationship was close to previous results. The intercolony variations in the oxygen isotope ratios were apparently caused by kinetic isotope effects related to variations in the skeletal growth rate rather than by species-specific variability or genetic differences within species. No correlation was found between skeletal carbon isotope ratios and temperature. The carbon isotope ratios showed significantly inverse correlation with linear growth rates, suggesting a kinetic isotope control at low growth rates. Observed intercolony variability in skeletal carbon isotope ratios (∼5‰) can be partly attributed to growth-rate-related kinetic isotope effects.     

Genetic algorithm for fitting a mixed Bingham distribution to 3D orientations: a tool for the statistical and paleostress analyses of fracture orientations

The clustering of fracture orientations is important for tectonic studies and for geotechnical engineering. In this study, a real‐coded genetic algorithm was adopted to fitting a mixed Bingham distribution to orientation data by maximizing the log‐likelihood function of the distribution. The maximization is a difficult problem, because the function has multimodality and singularity. It was found that the algorithm was effective for this problem. Given the orientations of dilational fractures, the present method determines not only the stress axes and stress ratio of each of the fracture groups but also the maximum non‐dimensionalized fluid pressure at the time of their formation. In addition, the software calculates the 95 % error ellipses of the concentration axes. The present method found that the orientations of ore veins of the Akenobe Mine, SW Japan, should be partitioned into three clusters. It is shown that two of the groups had distinctive Zn and Sn contents, and that the ore fluids had overpressures only slightly greater than the minimum principal stress at the time of the deposition of Zn‐ and Sn‐rich veins.     

Responses of phytoplankton and heterotrophic bacteria in the northwest subarctic Pacific to in situ iron fertilization as estimated by HPLC pigment analysis and flow cytometry

To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l⁻¹ in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l⁻¹ in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l⁻¹ at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment.     

Changes in the abundance and composition of picophytoplankton in relation to the occurrence of a Kyucho and a bottom intrusion in the Bungo Channel,Japan

The Bungo Channel in southwestern Japan receives both warm, called Kyucho, and cold deep-water intrusions (bottom intrusion) from the Pacific Ocean. Abundances of Prochlorococcus, Synechococcus, and eukaryotic picophytoplankton were monitored from 18 July to 17 August 2001 to clarify whether advected picophytoplankton from the Pacific Ocean can grow in the channel or not. Synechococcus cells were further discriminated into low- and high-PUB types according to their fluorescence property in flow cytometry. From 18 to 25 July, the water temperature decreased by 3 °C at a 5-m depth at all stations, indicating the occurrence of a bottom intrusion. From 25 July to 4 August, a Kyucho occurred and the water temperature rapidly increased. From 4 to 17 August, a bottom intrusion and a Kyucho both occurred twice, although the intensities were smaller than those occurring until 4 August. From 18 to 30 July, the abundance of both Prochlorococcus and a high-PUB type of Synechococcus drastically decreased because of a bottom intrusion; however, the abundances rapidly increased due to the advection by a Kyucho. These advected cells increased from 4 to 17 August in the channel and Kitanada Bay. Changes in the abundance of low-PUB type of Synechococcus and eukaryotic picophytoplankton were less noticeable than those in the abundance of Prochlorococcus and high-PUB type. The present study demonstrated that oceanic picophytoplankton advected by the Kyucho could grow in the channel. However, abundances of low-PUB type and eukaryotic picophytoplankton increased higher than those of Prochlorococcus and high-PUB type did. Thus, these oceanic phytoplankters will be excluded when Kyucho does not occur for a long time. The co-occurrence of various types of picophytoplankton found in the channel is probably achieved by both Kyucho event and their growth capability in the channel.     

Evidence for predominance of internal tidal currents in Sagami and Suruga bays

Tidal currents observed in a surface layer overlying deep water in Sagami and Suruga Bays frequently have large amplitude in summer and fall. Numerical experiments show that the current amplitude due to the surface tides is below 1.0 cm sec^–1 for the semidiurnal and diurnal constituents in the inner region of the two bays. The observed current amplitudes are larger than the calculated ones due to the surface tides. Therefore, the observed tidal currents are indicated to be due mainly to the internal tides. In addition, the semidiurnal currents dominate the diurnal currents in Sagami Bay, while the opposite occurs in Suruga Bay. These results suggest that the prevailing periods of the internal tides differ between the two bays,i.e., the internal tide has a semidiurnal period in Sagami Bay and a diurnal period in Suruga Bay.     

Difference in the prevailing periods of internal tides between Sagami and Suruga Bays: Numerical experiments

Current measurements in the surface layer in Sagami and Suruga Bays showed existence of significant tidal currents which are considered to be mainly due to internal tides (Inaba, 1982; Ohwaki,ea al., 1991). In addition, the prevailing period of the tidal currents is semidiurnal in Sagami Bay, but diurnal in Suruga Bay. To explain this difference in the prevailing, periods, numerical experiments were carried out using a two layer model. The internal tides are generated on the Izu Ridge outside the two bays. The semidiurnal internal tide propagates into Sagami Bay having characteristics of an internal inertia-gravity wave, while it propagates into Suruga Bay having characteristics of either an internal inertia-gravity wave or an internal Kelvin wave. The diurnal internal tide behaves only as an internal Kelvin wave, because the diurnal period is longer than the inertia period. Thus, the diurnal internal tide generated on the Izu Ridge can be propagated into Suruga Bay, while it cannot propagate into the inner region of Sagami Bay, though it is trapped around Oshima Island, which is located at the mouth of Sagami Bay. The difference in the propagation characteristics between the semidiurnal and diurnal internal tides can give a mechanism to explain the difference in the prevailing periods of the internal tides between Sagami and Suruga Bays.     

A theory of semigeostrophic gravity waves and its application to the intrusion of a density current along a coast

Semigeostrophic gravity waves associated with a coastal boundary current, which has finite and uniform potential vorticity and is bounded away from the coastline by a density front on the ocean surface, are investigated. It is shown that the semigeostrophic coastal current has two waves which are named here the Semigeostrophic Coastal Wave (SCW) and the Semigeostrophic Frontal Wave (SFW). The SCW becomes an elementary Kelvin wave at some limit while the SFW is caused by the existence of the surface density front. The SCW appears mainly as variations in the upper layer depth at the coast and as alongshore velocity at the density front. On the other hand, the SFW appears mainly as variations in the width of the current. When the weak nonlinearity and ageostrophic effect are included, these semigeostrophic gravity waves satisfy the Kortweg- de Vries equation, which suggests that the local changes in the width and/or velocity of the semigeostrophic coastal current propagate as wave-like disturbances.     

Jellyfish patch formation investigated by aerial photography and drifter experiment

Jellyfish patch formation is investigated by conducting a drifter experiment combined with aerial photography of a sustained patch of the moon jellyfish in Hokezu Bay, Japan. Jellyfish patches are aggregations of individuals that are caused by a combination of swimming (active influence) and advection by currents (passive influence). The drifter experiment involved the injection of 49 drifters around a distinct surface patch of jellyfish within an area of approximately 300 m × 300 m. The drifters’ motion, caused only by the passive influence, was recorded in a series of 38 aerial photographs taken over approximately 1 h. The ambient uniform current field larger than the patch scale was estimated from the movement of the centroid position of drifters, while the distribution of horizontal divergence and relative vorticity around the patch was estimated from the time-derivative in areas of triangles formed by the drifters. The centroid positions of both drifters and patches moved stably toward the bay head at different speeds. The difference vector between the patch and drifter centroids was directed to the sun, and was opposite to the ambient current. The distributions of vorticity and divergence around patches exhibited inhomogeneity within the patch scale, and the drifters in this nonuniform current field aggregated near the convergence area within 1 h. The results suggest that horizontal patch formation is predominantly influenced by passive factors at the surface of Hokezu Bay. Furthermore, the upward swimming against downwelling may make sustained patch in surface layer.