全文获取类型
收费全文 | 6257篇 |
免费 | 727篇 |
国内免费 | 1094篇 |
学科分类
生物科学 | 8078篇 |
出版年
2024年 | 28篇 |
2023年 | 113篇 |
2022年 | 277篇 |
2021年 | 397篇 |
2020年 | 304篇 |
2019年 | 378篇 |
2018年 | 353篇 |
2017年 | 277篇 |
2016年 | 312篇 |
2015年 | 527篇 |
2014年 | 565篇 |
2013年 | 597篇 |
2012年 | 732篇 |
2011年 | 629篇 |
2010年 | 407篇 |
2009年 | 324篇 |
2008年 | 363篇 |
2007年 | 272篇 |
2006年 | 242篇 |
2005年 | 204篇 |
2004年 | 161篇 |
2003年 | 129篇 |
2002年 | 115篇 |
2001年 | 67篇 |
2000年 | 61篇 |
1999年 | 51篇 |
1998年 | 33篇 |
1997年 | 24篇 |
1996年 | 13篇 |
1995年 | 16篇 |
1994年 | 25篇 |
1993年 | 16篇 |
1992年 | 14篇 |
1991年 | 10篇 |
1990年 | 7篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1970年 | 1篇 |
1950年 | 1篇 |
排序方式: 共有8078条查询结果,搜索用时 0 毫秒
1.
DNaseⅠ超敏感位点的研究能够发现潜在的调控基因转录活化的位点,比较正常人外周血有核细胞,淋巴瘤细胞株P3HR1和人鼻咽癌低分化磷癌细胞株HOnE1和HNE2中Ha-ras-1瘤基因的DNaseⅠ超敏感位点发现,只有HONE1和HNE2细胞基因组中存在一个DNaseⅠ超敏感位点,位于第一个外显子上游0.37kb处,上述结果提示正常白细胞和P3HR1细胞中Ha-ras-1基因处于失活状态,而在鼻咽癌细胞基因组中则处于活化状态,它的活化可能与0.37kb处的DNA序列有密切的关系。 相似文献
2.
Parthenogenetic sporophytes were obtained from three strains of Laminaria japonica Areschoug. These sporophytes grew to maturity in the sea, producine spores that all grew into female gametophytes. These female gametophytes gave rise to another generation of parthenogenetic sporophytes during the next year, so that by the year 1990 parthenogenetic sporophytes had been cultivated for 12, 9, and 7 generations, respectively, for the three strains. When female gametophytes from parthenogenetic sporophytes were combined with normal male gametophytes, normal sporophytes that reproduced and gave rise to both female and male gametophytes were obtained. The parthenogenetic sporophytes were shorter and narrower than the normal sporophytes of the same strain. Chromosome counts on mature sporophytes showed that normal sporophytes (from fertilized eggs) were diploid (2n = approximately 40) and that the spores they produced were haploid (n = approximately 20), while nuclei from both somatic and sporangial cells in parthenogenetic sporophytes were haploid. All gametophytes were haploid. Young sporophytes derived from cultures with both female and male gametophytes were diploid, while young, sporophytes obtained from female gametophytes from parthenogenetic sporophytes had haploid, diploid, or polyploidy chromosome numbers. Polyploidy was associated with abnormal cell shapes. The presence of haploid parthenogenetic sporophytes should be use in breeding kelp strains with useful characteristics, since the sporophyte phenotype is expressed from a haploid genotype which can be more readily selected. 相似文献
3.
4.
R T Mason J P Coghlan D A Denton D W Fei B A Scoggins J A Whitworth 《Prostaglandins》1984,27(4):527-534
The haemodynamic and renin responses to prostacyclin (PGI2) infusion were examined in sheep during sodium depletion and dietary sodium restriction. The haemodynamic effects of PGI2 infusion in sodium depleted and sodium restricted sheep were similar to those obtained in the sodium replete animal. The renin proportionate response to PGI2 was not altered by sodium restriction but blunted by sodium depletion, compatible with the hypothesis that endogenous PGI2 is high in Na depletion. 相似文献
5.
了解野放丹顶鹤的运动模式、家域和栖息地选择的时间节律特征对丹顶鹤种群保护和栖息地管理尤为重要。基于GPS-GSM跟踪数据,综合运用3S技术、动态布朗桥模型、栖息地选择指数,研究了盐城海滨湿地野放丹顶鹤在不同生活周期的活动节律、家域的面积和重叠指数,以及栖息地选择。结果表明:(1)丹顶鹤日活动节律具有明显的周期性特征。丹顶鹤活动强度:育成期>越冬期>孵化期>育雏期,孵化期和育雏期日间活动强度平稳,育成期和越冬期呈“双峰”模式。(2)丹顶鹤95%家域面积均值介于(111.18±22.15)hm2—(621.28±105.77)hm2,育成期((621.28±105.77) hm2)>育雏期((226.83±54.86) hm2)>孵化期((112.40±7.72) hm2)>越冬期((111.18±22.15) hm2);核心家域面积均值介于(0.53±0.26)—(45.78±6.66) hm2,育成... 相似文献
6.
7.
8.
9.
Choline is an essential nutrient for cell survival and proliferation, however, the expression and function of choline transporters have not been well identified in cancer. In this study, we detected the mRNA and protein expression of organic cation transporter OCT3, carnitine/cation transporters OCTN 1 and OCTN2, and choline transporter-like protein CTL1 in human lung adenocarcinoma cell lines A549, H 1299 and SPC-A-1. Their expression pattern was further confirmed in 25 human primary adenocarcinoma tissues. The choline uptake in these cell lines was significantly blocked by CTL1 inhibitor, but only partially inhibited by OCT or OCTN inhibitors. The efficacy of these inhibitors on cell proliferation is closely correlated with their abilities to block choline transport. Under the native expression of these transporters, the total choline uptake was notably blocked by specific PI3K/AKT inhibitors. These results describe the expression of choline transporters and their relevant function in cell proliferation of human lung adenocarcinoma, thus providing a potential "choline-starvation" strategy of cancer interference through targeting choline transporters, especially CTL1. 相似文献
10.
Ma YM Tao RY Liu Q Li J Tian JY Zhang XL Xiao ZY Ye F 《Molecular and cellular biochemistry》2011,357(1-2):65-72
PTP1B is a negative regulator of insulin signaling pathway. This study investigated the effects of compound CCF06240, a PTP1B inhibitor, on insulin sensitivity and lipid metabolic abnormalities in vivo and in vitro, respectively. The insulin resistant IRM mouse model was induced by HFD. The responses to insulin were determined by OGTT, ITT, and hyperinsulinemic-euglycemic clamp test. The body weight and the levels of serum TC and TG were measured to estimate the lipid metabolism in vivo. Recombinant human GST-PTP1B protein was used to measure the inhibition of CCF06240 on PTP1B activity. The hepatocyte lipid accumulation was induced by high concentrations of FFA and insulin in HepG(2) cells, and evaluated by the Oil Red O method. In IRM mice, the insulin resistance was improved; the body weight and the levels of TC and TG were also reduced by oral CCF06240 administration. In lipid accumulated model cells, CCF06240 was found to reverse the increased PTP1B activity, enhance the insulin-induced tyrosine phosphorylation in insulin signaling pathway, attenuate the FFA-insulin-induced cellular lipid accumulation, and down-regulate the expressions of genes related fatty acid synthesis. These results demonstrated that the PTP1B inhibitor, compound CCF06240, could increase insulin sensitivity through the regulation of insulin signaling pathway, and decrease FFA-insulin-induced hepatocytes lipid accumulation by reducing fatty acid syntheses. 相似文献