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排序方式: 共有225条查询结果,搜索用时 15 毫秒
1.
Gaelle Jan Violaine Delorme Nehm Saksouk Marie Abrivard Virginie Gonzalez Xavier Cayla Mohamed-Ali Hakimi Isabelle Tardieux 《Microbes and infection / Institut Pasteur》2009,11(12):935-945
Toxoplasma gondii is a human protozoan parasite that belongs to the phylum of Apicomplexa and causes toxoplasmosis. As the other members of this phylum, T. gondii obligatory multiplies within a host cell by a peculiar type of mitosis that leads to daughter cell assembly within a mother cell. Although parasite growth and virulence have been linked for years, few molecules controlling mitosis have been yet identified and they include a couple of kinases but not the counteracting phosphatases. Here, we report that in contrast to other animal cells, type 2C is by far the major type of serine threonine phosphatase activity both in extracellular and in intracellular dividing parasites. Using wild type and transgenic parasites, we characterized the 37 kDa TgPP2C molecule as an abundant cytoplasmic and nuclear enzyme with activity being under tight regulation. In addition, we showed that the increase in TgPP2C activity significantly affected parasite growth by impairing cytokinesis while nuclear division still occurred. This study supports for the first time that type 2C protein phosphatase is an important regulator of cell growth in T. gondii. 相似文献
2.
Valérie Toulon Hervé Sentenac Jean-Baptiste Thibaud André Soler David Clarkson Claude Grignon 《Planta》1989,179(2):235-241
The effect of HCO
3
-
on ion absorption by young corn roots was studied in conditions allowing the independent control of both the pH of uptake solution and the CO2 partial pressure in air bubbled through the solution. The surface pH shift in the vicinity of the outer surface of the plasmalemma induced by active H+ excretion was estimated using the initial uptake rate of acetic acid as a pH probe (Sentenac and Grignon (1987) Plant Physiol. 84, 1367). Acetic acid and orthophosphate uptake rates and NO
3
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accumulation were slowed down, while 86Rb+ uptake and K+ accumulation rates were increased by HCO
3
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. These effects were similar to those induced by 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid/2-amino-2-(hydroxymethyl)-1,3-propanediol (Hepes-Tris). They were more pronounced when the H+ excretion was strong, were rapidly reversible and were not additive to those of Hepes-Tris. The hypothesis is advanced that the buffering system CO2/H2CO3/HCO
3
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accelerated the diffusion of equivalent H+ inside the cell wall towards the medium. This attenuated the surface pH shift in the vicinity the plasma membrane and affected the coupling between the proton pump and cotransport systems.Abbreviations FW
fresh weight
- Hepes
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- Jaa
acetic acid influx
- JK
+
K+ influx
- JPi
orthophosphate influx
- Mes
2-(N-morpholino)ethanesulfonic acid
- pCO2
CO2 partial pressure
- Tris
2-amino-2-(hydroxymethyl)-1,3-propanediol 相似文献
3.
Incontinentia pigmenti: Xp breakpoint is not the same in a case of r(X) and in X/autosome translocations 总被引:3,自引:0,他引:3
A Sefiani S Heuertz C Turleau D Thibaud J de Grouchy M C Hors-Cayla 《Annales de génétique》1989,32(3):149-151
X-specific DNA probes were used to characterize the r(X) of a 45,X/46,X,r(X) female patient with Incontinentia pigmenti. It was found to be of maternal origin. Breakpoints were shown to be in or distal to p11.22 and between q12.2 and q13.1. When considering all known cases of Incontinentia pigmenti and X rearrangements at least four different break sites on the X have been shown. 相似文献
4.
Garcia A Cayla X Guergnon J Dessauge F Hospital V Rebollo MP Fleischer A Rebollo A 《Biochimie》2003,85(8):721-726
The reversible phosphorylation of proteins controlled by protein kinases and protein phosphatases is a major mechanism that regulates a wide variety of cellular processes. In contrast to C. elegans, recent studies in mammalian cells have highlighted a major role of serine/threonine protein phosphorylation in apoptosis. To illustrate the importance of dephosphorylation processes in apoptosis, this review will focus on recent studies suggesting that the interaction of the serine/threonine protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) with certain regulators of the Bcl-2 family is critically involved in the control of apoptosis. 相似文献
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7.
Chimpanzees (Pan troglodytes) and bonobos (P. paniscus) are our closest living relatives, with the human lineage diverging from the Pan lineage only around five to seven Mya, but possibly as early as eight Mya.1–2 Chimpanzees and bonobos even share genetic similarities with humans that they do not share with each other.2 Given their close genetic relationship to humans, both Pan species represent crucial living models for reconstructing our last common ancestor (LCA) and identifying uniquely human features. Comparing the similarities and differences of the two Pan is thus essential for constructing balanced models of human evolution.3 相似文献
8.
Florian Villegas Daphné Lehalle Daniela Mayer Melanie Rittirsch Michael B. Stadler Marietta Zinner Daniel Olivieri Pierre Vabres Laurence Duplomb-Jego Eveline S.J.M. De Bont Yannis Duffourd Floor Duijkers Magali Avila David Geneviève Nada Houcinat Thibaud Jouan Paul Kuentz Klaske D. Lichtenbelt Joerg Betschinger 《Cell Stem Cell》2019,24(2):257-270.e8
9.
Guard cell inward K+ channel activity in arabidopsis involves expression of the twin channel subunits KAT1 and KAT2 总被引:1,自引:0,他引:1
Pilot G Lacombe B Gaymard F Cherel I Boucherez J Thibaud JB Sentenac H 《The Journal of biological chemistry》2001,276(5):3215-3221
Stomatal opening, which controls gas exchanges between plants and the atmosphere, results from an increase in turgor of the two guard cells that surround the pore of the stoma. KAT1 was the only inward K(+) channel shown to be expressed in Arabidopsis guard cells, where it was proposed to mediate a K(+) influx that enables stomatal opening. We report that another Arabidopsis K(+) channel, KAT2, is expressed in guard cells. More than KAT1, KAT2 displays functional features resembling those of native inward K(+) channels in guard cells. Coexpression in Xenopus oocytes and two-hybrid experiments indicated that KAT1 and KAT2 can form heteromultimeric channels. The data indicate that KAT2 plays a crucial role in the stomatal opening machinery. 相似文献
10.
The carved stele known as the “head of the tribe”, attributed to the Chalcolithic, erected at an altitude of 2290 m in the chaos of blocks in the Merveilles torrent in the Mont Bego region at Tende, was removed from its original standing place. Earth extracted from under the stele and sieved yielded a sickle blade in very fine and homogeneous Bedoulian pale biege translucid flint, pressure flaked on a heated core. The blade bears a light polish caused by cereal harvesting. This sickle blade is similar to those widely used in the southern Chassey culture (4300 to 3000 years before our era) but also sometimes in the Campaniform culture, during the ancient and middle Bronze age, like in Murée cave, in the Verdon gorges. The location of the sickle blade at the foot of the carved stele, known as the “head of the tribe”, is not just coincidental. It is highly probable that the blade was intentionally placed beside this rock. It is seemingly during a ritual ceremony that this sickle blade, probably still inserted in a wooden handle, was intentionally placed, in a propitious gesture or as an offering, beside the stele known as the “head of the tribe”. 相似文献