全文获取类型
收费全文 | 462篇 |
免费 | 36篇 |
学科分类
生物科学 | 498篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 4篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 18篇 |
2013年 | 37篇 |
2012年 | 26篇 |
2011年 | 24篇 |
2010年 | 19篇 |
2009年 | 16篇 |
2008年 | 22篇 |
2007年 | 26篇 |
2006年 | 28篇 |
2005年 | 22篇 |
2004年 | 21篇 |
2003年 | 34篇 |
2002年 | 27篇 |
2001年 | 10篇 |
2000年 | 9篇 |
1999年 | 12篇 |
1998年 | 11篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1993年 | 2篇 |
1992年 | 7篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1985年 | 2篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 2篇 |
1979年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1967年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有498条查询结果,搜索用时 15 毫秒
1.
Y Hamano H Kodama M Yanagisawa Y Haraguchi M Mori S Yokota 《The journal of histochemistry and cytochemistry》1988,36(1):29-35
We investigated light and electron microscopic localization of ornithine transcarbamylase (OTC) in rat intestinal mucosa. In the immunoblotting assay of OTC-related protein, a single protein band with a molecular weight of about 36,500 is observed in extracts of liver and small intestinal mucosa but is not observed in those of stomach and large intestine. For light microscopy, tissue slices of the digestive system were embedded in Epon and stained by using anti-bovine OTC rabbit IgG and the immunoenzyme technique. For electron microscopy, slices of these and the liver tissues were embedded in Lowicryl K4M and stained by the protein A-gold technique. By light microscopy, the absorptive epithelial cells of duodenum, jejunum, and ileum stained positively for OTC, but stomach, large intestine, rectum, and propria mucosa of small intestine were not stained. Electron microscopy showed that gold particles representing the antigenic sites for OTC were confined to the mitochondrial matrix of hepatocytes and small intestinal epithelial cells. However, the enzyme was detected in mitochondria of neither liver endothelial cells, submucosal cells of small intestine, nor large intestinal epithelial cells. Labeling density of mitochondria in the absorptive epithelial cells of duodenum, jejunum, and ileum was about half of that in liver cells. 相似文献
2.
Formation of Recombinants Between Nontransmissible Drug-Resistance Determinants and Transfer Factors 总被引:5,自引:2,他引:3 下载免费PDF全文
Susumu Mitsuhashi Mitsuo Kameda Kenji Harada Mitsue Suzuki 《Journal of bacteriology》1969,97(3):1520-1521
Noninfectious drug-resistance determinants acquired conjugal transmissibility by the formation of recombinants with transfer factors, suggesting the origin of R factors. 相似文献
3.
T Endo M Ikeda M Ohmori E Anzai K Haraguchi T Onaya 《Biochemical and biophysical research communications》1992,187(2):887-893
We have produced rabbit antibody against a synthetic peptide corresponding to N-terminal region of the extracellular domain of human thyrotropin receptor (hTSH-R) (N peptide, aminoacid residues 29-57). Western blot analysis revealed that N-peptide antibody recognized recombinant hTSH-R stably expressing in CHO-K1 cells as a mol. wt. about 104 kDa regardless in the presence or absence of disulfide-reducing agent. The band was not detected in untransfected CHO-K1 cells and no band was also stained by the antibody absorbed with N-peptide. In a reducing condition, the antibody also bound the rat receptor from FRTL5 cells as the same molecular size (104 kDa). These results clearly indicate that TSH-R is composed of a single subunit and that two subunit model for the TSH-R may reflect artifactual proteolytic cleavage of the receptor during membrane preparation. 相似文献
4.
S. G. Nugroho J. Lumbanraja H. Suprapto Sunyoto H. Haraguchi M. Kimura 《Plant and Soil》1996,181(2):287-293
Yearly and seasonal (rainy and dry seasons) variations of CH4 emission from a Sumatra paddy field were measured for 3 years. The mean CH4 emission rates during the growth period were in the range of 16.0–26.1 mg CH4 m-2 h-1 for the chemical fertilizer plots and 23.3–34.9 mg CH4 m-2 h-1 for the plots with rice straw application, respectively. The increase in the amounts of CH4 emission by rice straw application were from 1.3 to 1.6 times. There was no significant difference in the mean CH4 emission rates between rainy and dry seasons.Total amounts of CH4 emitted during the period of rice growth were in the ranges of 29.5–48.2 and 43.0–64.6 g CH4 m-2 for the plots applied with chemical fertilizer and those with rice straw application, respectively. Nearly the same amounts of CH4 were emitted in the first and second half of the growth period, irrespective of rice straw application. 相似文献
5.
A case of transient hypothyroidism in the course of hypokalemic myopathy is reported. A 69-year-old woman had severe muscle weakness and marked potassium deficiency associated with alkalosis during treatment with thiazide diuretics. The cause of muscle weakness proved to be hypokalemic myopathy confirmed by clinical findings and muscle biopsy. After the episode of hypokalemic myopathy, serum levels of thyroid hormone were lowered (T4; 3.8 micrograms/dl, T3; 54 ng/dl) and that of TSH was elevated (25.1 microU/ml). Antithyroid microsomal antibody was positive (1:25600) and anti-thyroglobulin antibody was negative. About one month after potassium supplement, her thyroid functions returned to normal, along with normalization of serum potassium level. This is the first documented case report of hypokalemic myopathy accompanied by transient hypothyroidism in a patient with autoimmune thyroiditis. We suggest that this transient hypothyroidism might be induced by hypokalemia during the course of autoimmune thyroiditis. 相似文献
6.
Mitsue Shibata Satoshi Kikuchi Hiroshi Tanaka Masahiro Sueyoshi Hiroshi Yoshimaru Kaoru Niiyama 《Ecological Research》2009,24(1):1-9
The effects of local population density, sex morph [protogynous (PG) or protandrous (PA)], and individual tree size on the
demographic processes of seed production were investigated in a heterodichogamous maple, Acer mono Maxim. var. Marmoratum (Nichols.) Hara f. dissectum, in a temperate forest of Japan. As the distance from conspecific reproductive adults increased, the percentage of immature
seed fall and empty seeds increased significantly, indicating higher pollination success along with local population density.
Although the difference was not distinct, pollination success was affected by the local population density of the reciprocal
sex morph rather than that of both sex morphs. The trees at higher local population density sites suffered higher seed mortality
due to predation and decay, and tended to produce smaller seeds. Thus, the impacts of local population density operated both
positively and negatively on reproduction. As a factor of individual traits, tree size scarcely affected any demographic processes.
On the other hand, sex morph did affect pollination success. Trees of PG type had lower immature seed fall than those of PA
type, suggesting that the former has higher efficiency of pollen acceptance than the latter. The results on seed demography
presented here partly support previous suggestions that heterodichogamous plants exhibit reciprocal cross-pollination and
gender specialization as reproductive traits. 相似文献
7.
8.
Nomura M Katayama K Nishimura A Ishida Y Ohta S Komari T Miyao-Tokutomi M Tajima S Matsuoka M 《Plant molecular biology》2000,44(1):99-106
The small subunit of ribulose-bisphosphate carboxylase (Rubisco), encoded by rbcS, is essential for photosynthesis in both C3 and C4 plants, even though the cell specificity of rbcS expression is different between C3 and C4 plants. The C3 rbcS is specifically expressed in mesophyll cells, while the C4 rbcS is expressed in bundle sheath cells, and not mesophyll cells. Two chimeric genes were constructed consisting of the structural gene encoding -glucuronidase (GUS) controlled by the two promoters from maize (C4) and rice (C3) rbcS genes. These constructs were introduced into a C4 plant, maize. Both chimeric genes were specifically expressed in photosynthetic organs, such as leaf blade, but not in non-photosynthetic organs. The expressions of the genes were also regulated by light. However, the rice promoter drove the GUS activity mainly in mesophyll cells and relatively low in bundle sheath cells, while the maize rbcS promoter induced the activity specifically in bundle sheath cells. These results suggest that the rice promoter contains some cis-acting elements responding in an organ-pecific and light-inducible regulation manner in maize but does not contain element(s) for bundle sheath cell-specific expression, while the maize promoter does contain such element(s). Based on this result, we discuss the similarities and differences between the rice (C3) and maize (C4) rbcS promoter in terms of the evolution of the C4 photosynthetic gene. 相似文献
9.
nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development 总被引:2,自引:0,他引:2
Haraguchi S Tsuda M Kitajima S Sasaoka Y Nomura-Kitabayashid A Kurokawa K Saga Y 《Mechanisms of development》2003,120(6):721-731
A mouse nanos (nanos1) gene was cloned and its function was examined by generating a gene-knockout mouse. The nanos1 gene encodes an RNA-binding protein, which contains a putative zinc-finger motif that exhibits similarity with other nanos-class genes in vertebrates and invertebrates. Although nanos1 is not detected in primordial germ cells, it is observed in seminiferous tubules of mature testis. Interestingly, maternally expressed nanos1 is observed in substantial amounts in oocytes, but the amount of maternal RNA is rapidly reduced after fertilization, and the transient zygotic nanos1 expression is observed in eight-cell embryos. At 12.5 days postcoitum, nanos1 is re-expressed in the central nervous system and the expression continues in the adult brain, in which the hippocampal formation is the predominant region. The nanos1 -deficient mice develop to term without any detectable abnormality and they are fertile. No significant neural defect is observed in terms of their behavior to date. 相似文献
10.
Kazuhiro Haraguchi Shigeru Saito Hiromichi Tanaka Tadashi Miyasaka 《Nucleosides, nucleotides & nucleic acids》2013,32(2-4):483-493
Abstract Pummerer-type rearrangement of uracil nucleosides having a phenylseleno group in the 2′-, 3′-, and 5′-positions took place upon oxidation with MCPBA in CH2Cl2 followed by treatment with acid anhydrides to yield α-acyloxyselenides. 相似文献