全文获取类型
收费全文 | 60篇 |
免费 | 4篇 |
学科分类
生物科学 | 64篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 4篇 |
2013年 | 1篇 |
2012年 | 1篇 |
2011年 | 6篇 |
2010年 | 3篇 |
2009年 | 3篇 |
2008年 | 3篇 |
2007年 | 6篇 |
2006年 | 1篇 |
2003年 | 1篇 |
2002年 | 1篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1991年 | 2篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有64条查询结果,搜索用时 9 毫秒
1.
S. M. Renaud H. C. Zhou D. L. Parry Luong-Van Thinh K. C. Woo 《Journal of applied phycology》1995,7(6):595-602
The effect of temperature from 10 °C to 35 °C on the growth, total lipid content, and fatty acid composition of three species of tropical marine microalgae, Isochrysis sp., Nitzschia closterium, N. paleacea (formerly frustulum), and the Tahitian Isochrysis sp. (T.ISO), was investigated.Cultures of N. closterium, Isochrysis sp. and T.ISO grew very slowly at 35 °C, while N. closterium did not grow at temperatures higher than 30 °C or lower than 20 °C. N. paleacea was low-temperature tolerant, with cells growing slowly at 10 °C. N. paleacea produced the highest percentage of lipids at 10 °C, while the other species produced maximum amounts of lipid at 20 °C. None of the species maintained high levels of polyunsaturated fatty acids (PUFAs) at high growth temperature and there was a significant inverse relationship between the percentage of PUFAs and temperature for N. paleacea. A curved relationship was found between temperature and percentage of PUFA for N. closterium and tropical Isochrysis sp., with the maximum production of PUFA at 25 °C and 20 °C, respectively. The two Nitzschia species produced higher levels of the essential fatty acid eicosapentaenoic acid [20:5(n-3)] at lower growth temperatures, but the two Isochrysis species had little change in percentage of 20:5(n-3) with temperature. Only T.ISO had the highest percentage of 22:6(n-3) at lowest growth temperature (11.4% total fatty acids at 10 °C).School of Mathematical and Physical SciencesAuthor for correspondence 相似文献
2.
3.
Jaegoo Kim Thinh Ha Quang Bao Yu-Kyong Shin Ki-Young Kim 《World journal of microbiology & biotechnology》2018,34(11):167
Candida albicans is a major invasive pathogen, and the development of strains resistant to conventional antifungal agents has been reported in recent years. We evaluated the antifungal activity of 44 compounds against Candida strains. Magnoflorine showed the highest growth inhibitory activity of the tested Candida strains, with a minimum inhibitory concentration (MIC) of 50 μg/mL based on microdilution antifungal susceptibility testing. Disk diffusion assay confirmed the antifungal activity of magnoflorine and revealed that this activity was stable over 3 days compared to those of berberine and cinnamaldehyde. Cytotoxicity testing showed that magnoflorine could potentially be used in a clinical setting because it didn’t have any toxicity to HaCaT cells even in 200 μg/mL of treatment. Magnoflorine at 50 μg/mL inhibited 55.91?±?7.17% of alpha-glucosidase activity which is required for normal cell wall composition and virulence of Candida albicans. Magnoflorine also reduced the formation of C. albicans’ biofilm. Combined treatment with magnoflorine and miconazole decreased the amount of miconazole required to kill various Candida albicans. Therefore, magnoflorine is a good candidate lead compound for novel antifungal agents. 相似文献
4.
Thinh T. Vu Nguyen Q. Hoa Anh Benjamin M. Rawson Dung V. Tran Hoa T. Nguyen Thinh N. Van 《American journal of primatology》2020,82(9):e23171
All gibbon species (Family: Hylobatidae) are considered threatened with extinction and recognized on the International Union for Conservation of Nature Red List of Threatened Species. Because gibbons are one of the most threatened families of primates, monitoring their status is now critically important. Long-term monitoring programs applying occupancy approaches, in addition to assessing occurrence probability, improves understanding of other population parameters such as site extinction or colonization probabilities, which elucidate temporal and spatial changes and are therefore important for guiding conservation efforts. In this study, we used multiple season occupancy models to monitor occurrence, extinction, and colonization probabilities for northern yellow-cheeked crested gibbon Nomascus annamensis in three adjacent protected areas in the Central Annamites mountain range, Vietnam. We collected data at 30 listening posts in 2012, 2014, and 2016 using the auditory point count method. Occurrence probabilities were highest in 2012 (0.74, confidence interval [CI]: 0.56–0.87) but slightly lower in 2014 (0.66, CI: 0.51–0.79) and 2016 (0.67, CI: 0.49–0.81). Extinction probabilities during the 2012–2014 and 2014–2016 intervals were 0.26 (0.14–0.44) and 0.25 (0.12–0.44), respectively. Colonization probabilities during 2012–2014 were 0.44 (0.19–0.73) and between 2014 and 2016 was 0.51 (0.26–0.75). Although local site extinctions have occurred, high recolonization probability helped to replenish the unoccupied sites and kept the occurrence probability stable. Long-term monitoring programs which use occurrence probability alone might not fully reveal the true dynamics of gibbon populations. We strongly recommend including multiple season occupancy models to monitor occurrence, extinction, and colonization probabilities in long-term gibbon monitoring programs. 相似文献
5.
Errors in replication or segregation lead to DNA damage, mutations, and aneuploidies. Consequently, cells monitor these events and delay progression through the cell cycle so repair precedes division. The DNA damage response (DDR), which monitors DNA integrity, and the spindle assembly checkpoint (SAC), which responds to defects in spindle attachment/tension during metaphase of mitosis and meiosis, are critical for preventing genome instability. Here we show that the DDR and SAC function together throughout the cell cycle to ensure genome integrity in C. elegans germ cells. Metaphase defects result in enrichment of SAC and DDR components to chromatin, and both SAC and DDR are required for metaphase delays. During persistent metaphase arrest following establishment of bi-oriented chromosomes, stability of the metaphase plate is compromised in the absence of DDR kinases ATR or CHK1 or SAC components, MAD1/MAD2, suggesting SAC functions in metaphase beyond its interactions with APC activator CDC20. In response to DNA damage, MAD2 and the histone variant CENPA become enriched at the nuclear periphery in a DDR-dependent manner. Further, depletion of either MAD1 or CENPA results in loss of peripherally associated damaged DNA. In contrast to a SAC-insensitive CDC20 mutant, germ cells deficient for SAC or CENPA cannot efficiently repair DNA damage, suggesting that SAC mediates DNA repair through CENPA interactions with the nuclear periphery. We also show that replication perturbations result in relocalization of MAD1/MAD2 in human cells, suggesting that the role of SAC in DNA repair is conserved. 相似文献
6.
Philip G Wong Michele A Glozak Thinh V Cao Cyrus Vaziri Edward Seto Mark G Alexandrow 《Cell cycle (Georgetown, Tex.)》2010,9(21):4351-4363
The efficiency of metazoan origins of DNA replication is known to be enhanced by histone acetylation near origins. Although this correlates with increased MCM recruitment, the mechanism by which such acetylation regulates MCM loading is unknown. We show here that Cdt1 induces large-scale chromatin decondensation that is required for MCM recruitment. This process occurs in G1, is suppressed by Geminin and requires HBO1 HAT activity and histone H4 modifications. HDAC11, which binds Cdt1 and replication origins during S phase, potently inhibits Cdt1-induced chromatin unfolding and re-replication, suppresses MCM loading and binds Cdt1 more efficiently in the presence of Geminin. We also demonstrate that chromatin at endogenous origins is more accessible in G1 relative to S phase. These results provide evidence that histone acetylation promotes MCM loading via enhanced chromatin accessibility. This process is regulated positively by Cdt1 and HBO1 in G1 and repressed by Geminin-HDAC11 association with Cdt1 in S phase and represents a novel form of replication licensing control.Key words: Cdt1, HBO1, HDAC11, chromatin, DNA replication 相似文献
7.
We report an experimental procedure that results in the immobilization of the motile Tetraselmis in an alginate bead, thereby providing clonal populations for ultrastructure research and greatly facilitating transmission
electron microscope (TEM) studies. The Prasinophyte Tetraselmis CS317, which is a potential candidate species for aquaculture, was used in our study. The cells were immobilized in Ca-alginate
beads and allowed to grow within the beads for 2 weeks. Each cell in the bead divided repeatedly, resulting in a dense clonal
population which could be easily distinguished under a compound microscope. Portions of the Tetraselmis-alginate beads containing the clonal populations were then used for TEM processing for a fine structure study without the
need for centrifugation. The normal TEM processing of microalgae by repeated centrifugations during processing or a centrifugation-agar
embedding mixture is very time consuming and unreliable due to the nature of the agar and, in the case of the motile Tetraselmis, the force of centrifugation required to sediment the cells. Our results revealed that the alginate did not interfere with
the fixation, embedding and sectioning, and the cells appeared to possess all of the structural characteristics of Tetraselmis cells, including the flagella apparatus. We conclude that immobilized Tetraselmis in alginate provides a simple experimental system for ultrastructural research.
Presented at the 6th Meeting of the Asian Pacific Society of Applied Phycology, Manila, Philippines. 相似文献
8.
Nguyen Hoang Loc Le Thi Thinh Moon-Sik Yang Tae-Geum Kim 《Biotechnology and Bioprocess Engineering》2011,16(3):576-580
The cholera toxin B subunit (CTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral
adjuvant that induces a strong immune response against co-administered or coupled antigens. A gene encoding CTB, which was
modified based on the optimized codon usage in the plant, was synthesized and fused to the endoplasmic reticulum retention
signal KDEL to enhance its expression level in plants. The synthetic CTB (sCTB) gene was introduced into a plant expression
vector adjacent to the CaMV 35S promoter, and was transformed into tomato using an Agrobacterium-mediated transformation method. The integration of the sCTB gene into the genomic DNA of transgenic plants was confirmed
by genomic DNA PCR amplification. The synthesis and assembly of CTB protein in transgenic plants was demonstrated through
immunoblot analysis and GM1-ELISA. The highest amount of CTB protein produced in transgenic tomatoes was approximately 0.9% of total soluble fruit protein
which was 10-fold greater than the previously 0.081%. GM1-ELISA indicated that plant-synthesized CTB protein bound specifically to GM1-gangliosides, suggesting that the CTB subunits formed active pentamers. 相似文献
9.
Seasonal Variation in the Chemical Composition of Tropical Australian Marine Macroalgae 总被引:1,自引:0,他引:1
The proximate chemical composition (ash, soluble carbohydrate, lipid and protein) was determined in 30 common species of tropical Australian marine macroalgae from Darwin Harbour (12∘26′S, 130∘51′E), in summer (hot and wet) and winter (cool and dry). There was a wide diversity of species in both seasons (19 species in summer and 20 species in winter). In most species, the major component was soluble carbohydrate (chlorophytes range 2.5–25.8% dry weight (dw), phaeophytes range 8.4–22.2% dw, rhodophytes range 18.7–39.2% dw) with significantly higher (p < 0.05) percentages only in winter season rhodophytes. Highest percentages of protein were found in rhodophytes collected in the summer (range 4.8–12.8% dw), with significantly lower percentages (p < 0.05) during winter. All species had lipid contents within the range 1.3–7.8% dw, with highest percentages in summer phaeophytes, but no significant differences between species or season. Most species had moderate to high ash contents (24.2–89.7% dw), with the highest percentages during summer. Compared with summer samples, macroalgae collected in winter had higher energy value and slightly lower percentages of inorganic matter. The variation of algal groups and chemical composition may influence the availability of the food source for the majority of herbivores, which in turn is likely to effect their ecology and community structure. 相似文献
10.
Le MT Wertheim HF Nguyen HD Taylor W Hoang PV Vuong CD Nguyen HL Nguyen HH Nguyen TQ Nguyen TV Van TD Ngoc BT Bui TN Nguyen BG Nguyen LT Luong ST Phan PH Pham HV Nguyen T Fox A Nguyen CV Do HQ Crusat M Farrar J Nguyen HT de Jong MD Horby P 《PloS one》2008,3(10):e3339