全文获取类型
收费全文 | 3319篇 |
免费 | 193篇 |
学科分类
生物科学 | 3512篇 |
出版年
2022年 | 18篇 |
2021年 | 23篇 |
2020年 | 18篇 |
2019年 | 30篇 |
2018年 | 36篇 |
2017年 | 46篇 |
2016年 | 67篇 |
2015年 | 78篇 |
2014年 | 115篇 |
2013年 | 167篇 |
2012年 | 151篇 |
2011年 | 177篇 |
2010年 | 116篇 |
2009年 | 112篇 |
2008年 | 182篇 |
2007年 | 185篇 |
2006年 | 186篇 |
2005年 | 181篇 |
2004年 | 218篇 |
2003年 | 179篇 |
2002年 | 205篇 |
2001年 | 103篇 |
2000年 | 89篇 |
1999年 | 89篇 |
1998年 | 45篇 |
1997年 | 35篇 |
1996年 | 29篇 |
1995年 | 18篇 |
1994年 | 28篇 |
1993年 | 35篇 |
1992年 | 73篇 |
1991年 | 47篇 |
1990年 | 42篇 |
1989年 | 40篇 |
1988年 | 32篇 |
1987年 | 28篇 |
1986年 | 32篇 |
1985年 | 34篇 |
1984年 | 26篇 |
1983年 | 15篇 |
1982年 | 18篇 |
1981年 | 15篇 |
1980年 | 14篇 |
1979年 | 19篇 |
1978年 | 21篇 |
1976年 | 15篇 |
1975年 | 12篇 |
1974年 | 9篇 |
1972年 | 10篇 |
1968年 | 10篇 |
排序方式: 共有3512条查询结果,搜索用时 0 毫秒
1.
2.
Structural studies of a human gamma 3 myeloma protein (Jir) bearing the allotypic marker Gm(st) 总被引:2,自引:0,他引:2
H Matsumoto S Ito T Miyazaki T Ohta 《Journal of immunology (Baltimore, Md. : 1950)》1983,131(4):1865-1870
The authors established the amino acid substitutions determining G3m(s) and G3m(t) specificities, which characterize Mongoloid populations, by sequence analysis of the Fc region of a myeloma protein (Jir). By comparing the amino acid sequences of the IgG3 (Jir) and the other IgG subclasses analyzed to date, it was found that G3m(s) was an isoallotype specified by an amino acid substitution at position 435; i.e., whereas the subclasses IgG1, IgG2, and IgG4 had histidine in common, G3m(s-) had arginine in this position. This was also confirmed by the observation that the Fc fragment in question bound to protein A. It was also established that the amino acid at position 379 of G3m(t-) IgG3 and the other subclasses was valine, whereas methionine in this position was specific for G3m(t+). In addition, the amino acids at position 339 of G3m(u-) IgG3 Jir was threonine, and at position 296 of G3m(g-) IgG3 Jir was tyrosine. These findings are not in accord with the hitherto postulated relations of alanine and phenylalanine to G3m(u-) and G3m(g-), respectively. Finally, this study showed that a large number of substitutions occurred at positions 384 through 389, which suggests that many specificities of the G3m(b) group occur on IgG3 proteins. 相似文献
3.
4.
K Miyazaki F Umenishi K Funahashi N Koshikawa H Yasumitsu M Umeda 《Biochemical and biophysical research communications》1992,185(3):852-859
Progelatinase A was purified as a complex with TIMP-2 from the conditioned medium of a human glioblastoma cell line. The TIMP-2/progelatinase complex was resistant to the activation by p-aminophenylmercuric acetic acid (APMA), and showed less than 10% of the activity of the TIMP-2-free active enzyme. When the complex was incubated with stromelysin in the presence of APMA, the 64-kDa progelatinase was effectively converted to the 57-kDa mature enzyme, increasing its gelatinolytic activity about 8-fold. These results suggest that stromelysin is a natural activator of TIMP-2-bound progelatinase A. 相似文献
5.
BACKGROUND: We report a successful pregnancy in a woman with severe ovarian dysfunction and infertility associated with a variant beta-subunit of luteinizing hormone (LH). METHOD/OUTCOME: A 35-year-old woman consulted our unit for infertility. Laparoscopy and ultrasonography showed obstruction of the right tube and ovulation from the right ovary only. Human menopausal gonadotrophin (hMG) therapy was used for six subsequent cycles, but did not result in conception. Subsequently, marked elevation of follicle-stimulating hormone (FSH) and testosterone, together with polycystic ovary (PCO) were noted. The patient failed to respond to ovarian stimulation by hMG. Severe ovarian dysfunction such as premature ovarian failure (POF) was strongly suspected. Sequence analysis of the LH beta-subunit gene indicated heterozygosity for point mutations Trp(8) to Arg(8) and Ile(15) to Thr(15) in the coding sequence. LH hypersecretion resembling that seen in PCO syndrome was observed. Induction of ovulation by hMG was successful in the first cycle in which the basal LH and FSH were well controlled with gonadotrophin-releasing hormone analog following estrogen-progesterone replacement. She conceived and delivered a healthy male infant at term. CONCLUSION: Clinicians should be clinically aware of patients with immunologically anomalous LH variant who might be at risk of developing ovarian failure within a relatively short time span. Pertinent treatment should be applied without delay in such cases. 相似文献
6.
Hydrogen peroxide was formed in isolated cell walls from Marchantiapolymorpha L. in the presence of MnCl2 by either NADH or NADPHoxidation. This reaction was stimulated by phenols such as 2,4-dichlorophenolor p-coumarate, suggesting a reaction similar to that proposedfor the last step of lignification in higher plant cells, althoughbryophytes have been reported to be devoid of lignin. (Received June 16, 1987; Accepted March 3, 1987) 相似文献
7.
8.
9.
F Suzuki S Miyazaki M Uozumi N Okamoto S Yamashita K Murakami Y Nakamura 《FEBS letters》1989,256(1-2):47-50
The release of prorenin as well as renin from rat renal slices was confirmed by a rat prorenin-prosegment ELISA system and an assay system for determining the renin activity. A significant increase of the prorenin release was found by adding rat submandibular gland extract to the slice medium, indicating the existence of a prorenin-releasing stimulator (PRS) in the extract. The pI and molecular mass of PRS were 8.5-8.7 and 28-30 kDa, respectively. The PRS was completely inactivated by boiling or a proteinase treatment. 相似文献
10.
Tatsuo Nakahara Makoto Hirano Takashi Matsumoto Toshihide Kuroki Yoshinori Tatebayashi Tetsuyuki Tsutsumi Kouji Nishiyama Hiroaki Ooboshi Kaoru Nakamura Hiroshi Yao Akio Shiraishi Michinori Waki Hideyuki Uchimura 《Neurochemical research》1990,15(6):609-611
DNA and RNA contents in 20 brain regions or nuclei of the rat were determined by a highly sensitive method using high-performance liquid chromatography with electrochemical detection. The high DNA and RNA contents were found in the hypothalamic nuclei, especially the median eminence-arcuate nucleus. These results may be available for the preparation of nucleic acids as the regional control. 相似文献