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1.
Dragan V. Vinterhalter 《Plant Cell, Tissue and Organ Culture》1989,17(1):13-19
Callus tissue was induced in young stem segments cultured on MS based media supplemented with 0.25–0.5 mg l-1 2, 4-D. Shoots were differentiated on media containing 0.5–1.0 mg l-1 BA and 0.5–2.0 mg l-1 IBA or 0.1–0.2 mg l-1 NAA. The same media were suitable for shoot multiplication. Shoot elongation and rooting were strongly inhibited by BA and stimulated by auxins IBA and NAA. Medium containing 0.5 mg l-1 IBA was optimal for rooting. Root elongation was stimulated by light and inhibited in darkness. Transfer of rooted plantlets to outdoor conditions was feasible and special hardening procedures were not required. Among more than 5000 plants produced by this procedure only 9 off-type plants with variegated leaves were found. 相似文献
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S.N. Khrapunov A.I. Dragan A.F. Protas G.D. Berdyshev 《International journal of biological macromolecules》1984,6(1):31-34
It was shown that the histone tetramer (H3-H4)2 fluorescence spectra were shifted by about 2 nm towards the long-wave region and had a larger halfwidth than the free tyrosine fluorescence spectra. Denaturation with 8 m urea resulted in a shift towards the short-wave region and a decrease in the halfwidth of the histone tetramer (H3-H4)2 tyrosine fluorescence spectra. Fluorescence quenching of the histone tetramer (H3-H4)2 by iodine ions was analysed by the Stern-Volmer equation. It was estimated that at 0.1 m NaCl and 0.3–0.8 m NaCl, 45% and 60% tyrosyl fluorescence, respectively, was quenched by I? ions. The results obtained suggests that histone tetramer (H3-H4)2 may have several structural forms distinguished by the amount of ‘exposed’ and ‘buried’ tyrosyls depending upon the conditions of the medium. 相似文献
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Lovorka Grgurevic Boris Macek Mladen Mercep Mislav Jelic Tomislav Smoljanovic Igor Erjavec Ivo Dumic-Cule Stefan Prgomet Dragan Durdevic Drazen Vnuk Marija Lipar Marko Stejskal Vera Kufner Jelena Brkljacic Drazen Maticic Slobodan Vukicevic 《Biochemical and biophysical research communications》2011,(1):80
Members of the astacin family of metalloproteinases such as human bone morphogenetic protein 1 (BMP-1) regulate morphogenesis by processing precursors to mature functional extracellular matrix (ECM) proteins and several growth factors including TGFβ, BMP2, BMP4 and GFD8. We have recently discovered that BMP1-3 isoform of the Bmp-1 gene circulates in the human plasma and is significantly increased in patients with acute bone fracture. We hypothesized that circulating BMP1-3 might have an important role in bone repair and serve as a novel bone biomarker. When administered systemically to rats with a long bone fracture and locally to rabbits with a critical size defect of the ulna, recombinant human BMP1-3 enhanced bone healing. In contrast, neutralization of the endogenous BMP1-3 by a specific polyclonal antibody delayed the bone union. Invitro BMP1-3 increased the expression of collagen type I and osteocalcin in MC3T3-E1 osteoblast like cells, and enhanced the formation of mineralized bone nodules from bone marrow mesenchymal stem cells. We suggest that BMP1-3 is a novel systemic regulator of bone repair. 相似文献
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Jelena Perovic Cristina Silvar Janine Koenig Nils Stein Dragan Perovic Frank Ordon 《Molecular breeding : new strategies in plant improvement》2013,32(1):61-69
Recent advances in next-generation sequencing techniques and the development of genomics resources for crop plants with large genomes allow the detection of a large number of single nucleotide polymorphisms (SNPs) and their use in a high-throughput manner. However, such large numbers of SNPs are on the one hand not needed in some plant breeding projects and on the other hand not affordable in some cases, raising the need for fast and low-cost innovative techniques for marker detection. In marker selection in plant breeding programs, cleaved amplified polymorphic sequence (CAPS) markers still play a significant role as a complement to other high-throughput methods for SNP genotyping. New methods focusing on the acceleration of CAPS-based genotyping are therefore highly desirable. The combination of the classical CAPS method and a M13-tailed primer multiplexing assay was used to develop an agarose-gel-free protocol for the analysis of SNPs via restriction enzyme digestion. PCR products were fluorescence-labeled with a universal M13 primer and subsequently digested with the appropriate restriction endonuclease. After mixing differently labeled products, they were detected in a capillary electrophoresis system. This method allowed the cost-effective genotyping of several SNPs in barley in a multiplexed manner at an overall low cost in a short period of time. This new method was efficiently combined with the simultaneous detection of simple sequence repeats in the same electrophoresis run, resulting in a procedure well suited for marker-based selection procedures, genotyping of mapping populations and the assay of genetic diversity. 相似文献
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Jakovljevic Biljana Nikolic Turnic Tamara Jeremic Nevena Jeremic Jovana Bradic Jovana Ravic Marko Jakovljevic Vladimir Lj. Jelic Djordje Radovanovic Dragan Pechanova Olga Zivkovic Vladimir 《Molecular and cellular biochemistry》2019,453(1-2):111-119
Molecular and Cellular Biochemistry - Melatonin is a crucial neurohormone synthesized in the pineal gland that influences the physiology of animals. The molecular mechanism of norepinephrine... 相似文献
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Marko Dragas Igor Koncar Dragan Opacic Nikola Ilic Zivan Maksimovic Miroslav Markovic Marko Ercegovac Tatjana Simic Marija Pljesa-Ercegovac Lazar Davidovic 《PloS one》2015,10(4)